Human cytomegalovirus with IE-2 (UL122) deleted fails to express early lytic genes

Journal of Virology

Volumn 75, Issue 4, 2001, Pages 1870-1878

  Marchini, A ^a   Liu, H ^a   Zhu, H ^a  

^a RUTGERS NEW JERSEY MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMEDIATE EARLY PROTEIN; MESSENGER RNA; VIRUS RNA;

ARTICLE; ESCHERICHIA COLI; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; IMMEDIATE EARLY GENE; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PREPUCE; PRIORITY JOURNAL; PROMOTER REGION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSACTIVATION; VIRUS CULTURE; VIRUS INFECTIVITY; VIRUS REPLICATION;

ANTIGENS, VIRAL; CELL LINE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; CLONING, MOLECULAR; CONJUGATION, GENETIC; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; GENE DELETION; GENOME, VIRAL; HUMANS; IMMEDIATE-EARLY PROTEINS; MEMBRANE GLYCOPROTEINS; PLAQUE ASSAY; TRANS-ACTIVATORS; TRANSFECTION; VIRAL ENVELOPE PROTEINS; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 0035132107     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.4.1870-1878.2001     Document Type: Article

References (41)

1. Human cytomegalovirus tegument protein pp71 (ppUL82) enhances the infectivity of viral DNA and accelerates the infectious cycle
2. An isoform variant of the cytomegalovirus immediate-early auto repressor functions as a transcriptional activator
3. Cloning of the human cytomegalovirus (HCMV) genome as an infectious bacterial artificial chromosome in Escherichia coli: A new approach for construction of HCMV mutants
4. Retinoblastoma protein recruits histone deacetylase to repress transcription
5. Infectious clones of herpesviruses: A new approach for understanding viral gene function
6. Cytomegalovirus
7. The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding, and interacts with TBP and transcription factor TFIIB via regions of IE2 required for transcriptional regulation
8. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169
9. Longitudinal investigation of hearing disorders in children with congenital cytomegalovirus
10. Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons
11. Cloning of the complete human cytomegalovirus genome in cosmids
12. Exploitation of cellular signaling and regulatory pathways by human cytomegalovirus
13. Progressive and fluctuating sensorineural hearing loss in children with asymptomatic congenital cytomegalovirus infection
14. Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant
15. Functional interaction between the HCMV IE2 transactivator and the retinoblastoma protein
16. The human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a TATA box-dependent mechanism and interacts directly with TFIID
17. A cluster of dispensable genes within the human cytomegalovirus genome short component: IRS1, US1 through US5, and the US6 family
18. Replacement mutagenesis of the human cytomegalovirus genome: US10 and US11 gene products are nonessential
19. Defined large-scale alterations of the human cytomegalovirus genome constructed by cotransfection of overlapping cosmids
20. A 15-kilobase-pair region of the human cytomegalovirus genome which includes US1 through US13 is dispensable for growth in cell culture
21. Functional interaction between the human cytomegalovirus 86-kilodalton IE2 protein and the cellular transcription factor CREB
22. Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1 cis-acting elements
23. Cytomegaloviruses and their replication
24. A deletion mutant in the human cytomegalovirus gene encoding IE1 (491aa) is replication defective due to a failure in autoregulation
25. The human cytomegalovirus IE86 protein can block cell cycle progression after inducing transition into the S phase of permissive cells
26. Human cytomegalovirus UL36 protein is dispensable for viral replication in cultured cells
27. Global AIDS epidemic: Time to turn the tide
28. The functionally active IE2 immediate-early regulatory protein of human cytomegalovirus is an 80-kilodalton polypeptide that contains two distinct activator domains and a duplicated nuclear localization signal
29. Abundant 5 kb RNA of human cytomegalovirus without a major translational reading frame
30. Perinatal herpesvirus infections. Herpes simplex, varicella, and cytomegalovirus
31. The human cytomegalovirus IE2 86-kilodalton protein interacts with an early gene promoter via site-specific DNA binding and protein-protein associations
32. Construction and transposon mutagenesis in Escherichia coli of a full-length infectious clone of pseudorabies virus, an alphaherpesvirus
33. Transactivation by the human cytomegalovirus IE2 86-kilodalton protein requires a domain that binds to both the TATA box-binding protein and the retinoblastoma protein
34. Potential role of human cytomegalovirus and p53 interaction in coronary restenosis
35. Temporal patterns of human cytomegalovirus transcription: Mapping the viral RNAs synthesized at immediate early, early, and late times after infection
36. Transcription factor Sp1 mediates cell-specific trans-activation of the human cytomegalovirus DNA polymerase gene promoter by immediate-early protein IE86 in glioblastoma U373MG cells
37. Active transcriptional repression by the Rb-E2F complex mediates G1 arrest triggered by p16INK4a, TGFβ, and contact inhibition
38. Human cytomegalovirus IE1 and IE2 proteins block apoptosis