Lysine substitutions convert a bacterial-agglutinating peptide into a bactericidal peptide that retains anti-lipopolysaccharide activity and low hemolytic activity

Peptides

Volumn 35, Issue 2, 2012, Pages 231-238

  Abdolhosseini, Mahsa ^a,b   Nandula, Seshagiri R ^a   Song, Jonathan ^a   Hirt, Helmut ^a   Gorr, Sven Ulrik ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF PENNSYLVANIA SCHOOL OF DENTAL MEDICINE   (United States)

Author keywords

Agglutination; Bactericidal; BPIFA2; Hemolysis; Lipopolysaccharide; LPS

Indexed keywords

ALANINE; BACTERICIDE; GLYCYLLYSYLISOLEUCYLISOLEUCYLLYSYLLEUCYLLYSYLALANYLSERYLLEUCYLLYSINELEUCYLLEUCINAMIDE; HYDROXYL GROUP; LIPOPOLYSACCHARIDE; LYSINE; PEPTIDE GL13NH2; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBACTERIAL ACTIVITY; ANTIGEN ANTIBODY REACTION; ANTIINFLAMMATORY ACTIVITY; ARTICLE; BACTERICIDAL ACTIVITY; BLOOD TOXICITY; CONTROLLED STUDY; DRUG ACTIVITY; ESCHERICHIA COLI; HEMOLYSIS; HUMAN; HUMAN CELL; MALE; MOUSE; NONHUMAN; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SALIVA; SEPSIS; STREPTOCOCCUS GORDONII; VIRUS HEMAGGLUTINATION;

AGGLUTINATION; AMINO ACID SUBSTITUTION; ANIMALS; ANTI-BACTERIAL AGENTS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; ESCHERICHIA COLI; HEMOLYSIS; LIPOPOLYSACCHARIDES; LYSINE; MALE; MICE; MICE, INBRED C57BL; PEPTIDE FRAGMENTS; PORPHYROMONAS GINGIVALIS; PSEUDOMONAS AERUGINOSA; STREPTOCOCCUS GORDONII;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MUS; NEGIBACTERIA; PORPHYROMONAS GINGIVALIS; POSIBACTERIA; PSEUDOMONAS AERUGINOSA; STREPTOCOCCUS GORDONII;

EID: 84861188442     PISSN: 01969781     EISSN: 18735169     Source Type: Journal    
DOI: 10.1016/j.peptides.2012.03.017     Document Type: Article

References (26)

1. M. Abdolhosseini, J.B. Sotsky, A. Shelar, P.B. Joyce, and S.-U. Gorr Human parotid secretory protein is a lipopolysaccharide-binding protein: identification of an anti-inflammatory peptide domain Mol Cell Biochem 359 2011 1 8
2. J.G. Beeley, R. Eason, and D.H. Snow Isolation and characterization of latherin, a surface-active protein from horse sweat Biochem J 235 1986 645 650 (Pubitemid 16071971)
3. C.D. Bingle, and C.J. Craven PLUNC: a novel family of candidate host defence proteins expressed in the upper airways and nasopharynx Hum Mol Genet 11 2002 937 943 (Pubitemid 34449781)
4. C.D. Bingle, and S.-U. Gorr Host defense in oral and airway epithelia: chromosome 20 contributes a new protein family Int J Biochem Cell Biol 36 2004 2144 2152 (Pubitemid 39094438)
5. C.D. Bingle, R.L. Seal, and C.J. Craven Systematic nomenclature for the PLUNC/PSP/BSP30/SMGB proteins as a subfamily of the BPI fold-containing superfamily Biochem Soc Trans 39 2011 977 983
6. K. Brandenburg, J. Andrä, P. Garidel, and T. Gutsmann Peptide-based treatment of sepsis Appl Microbiol Biotechnol 90 2011 799 808
7. S.R. Coats, T.T. To, S. Jain, P.H. Braham, and R.P. Darveau Porphyromonas gingivalis resistance to polymyxin B is determined by the lipid A 4′-phosphatase, PGN-0524 Intl J Oral Sci 1 2009 126 135
8. C.A. Daep, E.A. Novak, R.J. Lamont, and D.R. Demuth Selective substitution of amino acids limits proteolytic cleavage and improves the bioactivity of an anti-biofilm peptide that targets the periodontal pathogen, Porphyromonas gingivalis Peptides 31 2010 2173 2178
9. P. Elsbach, and J. Weiss The bactericidal/permeability-increasing protein (BPI), a potent element in host-defense against gram-negative bacteria and lipopolysaccharide Immunobiology 187 1993 417 429 (Pubitemid 23150782)
10. L. Gakhar, J.A. Bartlett, J. Penterman, D. Mizrachi, P.K. Singh, and R.K. Mallampalli PLUNC is a novel airway surfactant protein with anti-biofilm activity PLoS One 5 2010 e9098
11. R. Gautier, D. Douguet, B. Antonny, and G. Drin HELIQUEST: a web server to screen sequences with specific alpha-helical properties Bioinformatics 24 2008 2101 2102
12. C. Geetha, S.G. Venkatesh, L. Bingle, C.D. Bingle, and S.U. Gorr Design, validation of anti-inflammatory peptides from human parotid secretory protein J Dent Res 84 2005 149 153 (Pubitemid 43822047)
13. C. Geetha, S.G. Venkatesh, B.H. Fasciotto Dunn, and S.-U. Gorr Expression and anti-bacterial activity of human parotid secretory protein (PSP) Biochem Soc Trans 31 2003 815 818 (Pubitemid 36981134)
14. S.-U. Gorr Antimicrobial peptides of the oral cavity Periodontol 2000 51 2009 152 180
15. S.-U. Gorr, M. Abdolhosseini, A. Shelar, and J. Sotsky Dual host-defense functions of SPLUNC2/PSP and synthetic peptides derived from the protein Biochem Soc Trans 39 2011 1028 1032
16. S.-U. Gorr, J.B. Sotsky, A.P. Shelar, and D.R. Demuth Design of bacteria-agglutinating peptides derived from Parotid Secretory Protein, a member of the Bactericidal/Permeability Increasing-like protein family Peptides 29 2008 2118 2127 (Pubitemid 50326971)
17. B.J.F. Keijser, E. Zaura, S.M. Huse, J.M.B.M. van der Vossen, F.H.J. Schuren, and R.C. Montijn Pyrosequencing analysis of the oral microflora of healthy adults J Dent Res 87 2008 1016 1020
18. L. Lukinskiene, Y. Liu, S.D. Reynolds, C. Steele, B.R. Stripp, and G.D. Leikauf Antimicrobial Activity of PLUNC Protects against Pseudomonas aeruginosa infection J Immunol 187 2011 382 390
19. S. Maher, and S. McClean Investigation of the cytotoxicity of eukaryotic and prokaryotic antimicrobial peptides in intestinal epithelial cells in vitro Biochem Pharmacol 71 2006 1289 1298
20. G. Maisetta, F.L. Brancatisano, S. Esin, M. Campa, and G. Batoni Gingipains produced by Porphyromonas gingivalis ATCC49417 degrade human-β-defensin 3 and affect peptide's antibacterial activity in vitro Peptides 32 2011 1073 1077
21. R.E. McDonald, R.I. Fleming, J.G. Beeley, D.L. Bovell, J.R. Lu, and X. Zhao Latherin: a surfactant protein of horse sweat and saliva PLoS One 4 2009 e5726
22. C. McInnes, L.H. Kondejewski, R.S. Hodges, and B.D. Sykes Development of the structural basis for antimicrobial and hemolytic activities of peptides based on gramicidin s and design of novel analogs using NMR spectroscopy J Biol Chem 275 2000 14287 14294 (Pubitemid 30339710)
23. C.P. Robinson, D.I. Bounous, C.E. Alford, K.H. Nguyen, J.M. Nanni, and A.B. Peck PSP expression in murine lacrimal glands and function as a bacteria binding protein in exocrine secretions Am J Physiol 272 1997 G863 G871
24. M.G. Scott, A.C.E. Vreugdenhil, W.A. Buurman, R.E.W. Hancock, M.R. Gold, and Cutting Edge Cationic antimicrobial peptides block the binding of lipopolysaccharide (LPS) to LPS binding protein J Immunol 164 2000 549 553 (Pubitemid 30043670)
25. G. Wang, X. Li, and Z. Wang APD2: the updated antimicrobial peptide database and its application in peptide design Nucleic Acids Res 37 2009 D933 D937
26. W.M. Weston, E.E. LeClair, W. Trzyna, K.M. McHugh, P. Nugent, and C.M. Lafferty Differential display identification of plunc, a novel gene expressed in embryonic palate, nasal epithelium, and adult lung J Biol Chem 274 1999 13698 13703