Hydrophobic interaction chromatography for purification of monoPEGylated RNase A

Journal of Chromatography A

Volumn 1242, Issue , 2012, Pages 11-16

  Mayolo Deloisa, Karla ^a,b   Lienqueo, Ma Elena ^b   Andrews, Barbara ^b   Rito Palomares, Marco ^a   Asenjo, Juan A ^b  

^a SCHOOL OF ENGINEERING AND SCIENCES   (Mexico)

^b UNIVERSITY OF CHILE   (Chile)

Author keywords

HIC; MonoPEGylated RNase A; PEGylated proteins; Purification

Indexed keywords

AMMONIUM SULPHATE; CHROMATOGRAPHIC METHODS; GRADIENT LENGTH; HIC; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; NATIVE PROTEINS; PEGYLATED PROTEINS; PEGYLATION; PLATE MODEL; PROTEIN HYDROPHOBICITY; RIBONUCLEASE A; RNASE A; SEPARATION PERFORMANCE; SIZE EXCLUSION; YIELD INCREASE;

AMMONIUM COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; POLYETHYLENE GLYCOLS; PURIFICATION; RESINS; SEPARATION; SODIUM CHLORIDE;

PROTEINS;

AMMONIUM SULFATE; BUTYLSEPHAROSE; MACROGOL; OCTYLSEPHAROSE; PHENYLSEPHAROSE; RIBONUCLEASE A; SEPHAROSE; SODIUM CHLORIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ENZYME PURIFICATION; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; HYDROPHOBICITY; PERFORMANCE MEASUREMENT SYSTEM; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; SEPARATION TECHNIQUE;

AMMONIUM SULFATE; ANIMALS; CATTLE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, LIQUID; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; POLYETHYLENE GLYCOLS; RIBONUCLEASE, PANCREATIC; SEPHAROSE;

EID: 84861185775     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2012.03.079     Document Type: Article

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