Separation of PEGylated from unmodified ribonuclease A using sepharose media

Separation and Purification Technology

Volumn 65, Issue 1, 2009, Pages 105-109

  Cisneros Ruiz, Mayra ^a   Mayolo Deloisa, Karla ^a   Przybycien, Todd M ^b   Rito Palomares, Marco ^a  

^a SCHOOL OF ENGINEERING AND SCIENCES   (Mexico)

^b CARNEGIE MELLON UNIVERSITY   (United States)

Author keywords

Chromatography media development; PEGylated proteins; Ribonuclease A

Indexed keywords

AMMONIUM COMPOUNDS; ANIMAL CELL CULTURE; CHROMATOGRAPHIC ANALYSIS; CHROMATOGRAPHY; ENZYMES; GEL PERMEATION CHROMATOGRAPHY; IONIC STRENGTH; PH; PHASE SEPARATION; PHOSPHATES; POLYETHYLENE GLYCOLS; PROTEINS; SEPARATION;

AMMONIUM SULFATES; AMPHIPHILIC; CHROMATOGRAPHIC METHODS; CHROMATOGRAPHY MEDIA DEVELOPMENT; GRADIENT ELUTIONS; HETEROGENEOUS POPULATIONS; MOBILE PHASIS; NATIVE PROTEINS; PEGYLATED PROTEINS; PEGYLATION; PHASE MODIFIERS; POTASSIUM PHOSPHATES; PROTEIN SEPARATIONS; RIBONUCLEASE A; SALT CONCENTRATIONS; SEPHAROSE; SEPHAROSE 4B;

PH EFFECTS;

EID: 58149288080     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2008.10.038     Document Type: Article

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