A new methanol-feeding strategy for the improved production of β-galactosidase in high cell-density fed-batch cultures of pichia Pastoris mut+ strains

Biotechnology and Bioprocess Engineering

Volumn 17, Issue 1, 2012, Pages 76-83

  Maghsoudi, Amir ^a   Hosseini, Safoura ^a   Shojaosadati, Seyed Abbas ^a   Vasheghani Farahani, Ebrahim ^a   Nosrati, Mohsen ^a   Bahrami, Ali ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

galactosidase; Alcohol oxidase; Fed batch fermentation; High celldensity; Methanol feeding; Pichia pastoris

Indexed keywords

ALCOHOL OXIDASE; FED-BATCH FERMENTATION; GALACTOSIDASES; HIGH CELLDENSITY; PICHIA PASTORIS;

AMMONIUM COMPOUNDS; BATCH CELL CULTURE; CHARGE DENSITY WAVES; FEEDING; FERMENTATION; FORMALDEHYDE; MAGNESIUM; RECOMBINANT PROTEINS; STRAIN; YEAST;

METHANOL;

ALCOHOL OXIDASE; AMMONIA; BETA GALACTOSIDASE; DISSOLVED OXYGEN; FORMALDEHYDE; FORMATE DEHYDROGENASE; MAGNESIUM; METHANOL; RECOMBINANT PROTEIN;

ARTICLE; ATOMIC ABSORPTION SPECTROMETRY; CONTROLLED STUDY; DRY WEIGHT; ENZYME ACTIVITY; ENZYME METABOLISM; ENZYME SYNTHESIS; FED BATCH CULTURE; FEEDING; FUNGAL STRAIN; FUNGUS CULTURE; FUNGUS GROWTH; GRAVIMETRY; GROWTH RATE; NONHUMAN; OXYGEN SATURATION; PICHIA PASTORIS; PROMOTER REGION; PROTEIN SYNTHESIS; SPECTROPHOTOMETRY;

PICHIA PASTORIS;

EID: 84861078285     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-011-0201-9     Document Type: Article

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