Overproduction of human interleukin-2 in recombinant Escherichia coli BL21 high-cell-density culture by the determination and optimization of essential amino acids using a simple stoichiometric model

Biotechnology and Applied Biochemistry

Volumn 54, Issue 1, 2009, Pages 31-39

  Yegane Sarkandy, S ^a   Farnoud, A M ^a   Shojaosadati, S A ^a   Khalilzadeh, R ^a   Sadeghyzadeh, M ^a   Ranjbar, B ^a   Babaeipour, V ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Essential amino acid; Full factorial design; Human interleukin 2 (IL 2); Recombinant Escherichia coli; Stoichiometric model

Indexed keywords

ESSENTIAL AMINO ACID; FULL FACTORIAL DESIGN; HUMAN INTERLEUKIN-2 (IL-2); RECOMBINANT ESCHERICHIA COLI; STOICHIOMETRIC MODEL;

AMINATION; AMINES; CELL CULTURE; CELL MEMBRANES; ESCHERICHIA COLI; FERMENTERS; MIXTURES; ORGANIC ACIDS; STOICHIOMETRY;

AMINO ACIDS;

ALANINE; ARGININE; ASPARAGINE; ASPARTIC ACID; CYSTEINE; GLUTAMIC ACID; GLUTAMINE; GLYCINE; HISTIDINE; INTERLEUKIN 2; ISOLEUCINE; LEUCINE; LYSINE; METHIONINE; PHENYLALANINE; PROLINE; SERINE; THREONINE; TRYPTOPHAN; TYROSINE; VALINE; ACETYL COENZYME A SYNTHETASE; ESSENTIAL AMINO ACID; GLUCOSE; LACTIC ACID; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BATCH CELL CULTURE; BATCH FERMENTATION; CELL DENSITY; CLINICAL EFFECTIVENESS; CONCENTRATION RESPONSE; ESCHERICHIA COLI; FED BATCH CULTURE; HUMAN; NONHUMAN; PLASMID; PROTEIN EXPRESSION; PROTEIN INTERACTION; STOICHIOMETRY; BIOLOGICAL MODEL; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; FERMENTATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION;

ESCHERICHIA COLI;

ACETATE-COA LIGASE; AMINO ACIDS, ESSENTIAL; BACTERIOLOGICAL TECHNIQUES; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GLUCOSE; GLUTAMINE; HUMANS; INTERLEUKIN-2; KINETICS; LACTIC ACID; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS;

EID: 70149100739     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20080300     Document Type: Article

References (29)

1. Schmidt, W., Schweighoffer, T., Herbst, E., Maass, G., Berger, M., Schilcher, F., Schaffner, G. and Brinstiel, M. L. (1995) Cancer Vaccines 92, 4711-4714
2. Velasco, I., Quereda, F., Bermejo, R., Campos, A. and Acien, P. (2007) J. Reprod. Immunol. 74, 124-132
3. Tsuji, T., Nakagawa, R., Sugimoto, N. and Fukuhara, K. (1987) Biochemistry 26, 3129-3134
4. Magnuson, N. S., Linzmaier, P. M., Reeves, R., An, G., Hay Glass, K. and Lee, J. M. (1998) Protein Expression Purif. 13, 45-52
5. Wang, Q., Ma, L., Zhou, M. Q., Liu, N. Y., Jing, S. R., Zou, Q. M. and Wang, X. M. (2005) Protein Expression Purif. 39, 131-136
6. Adams, J. E., Pena, M. T., Gillis, T. P., Williams, D. L., Adams, L. B. and Truman, R. W. (2005) Cytokine 32, 219-225
7. Kim, Y. S. and Cha, H. J. (2006) Enzyme Microb. Technol. 39, 399-406
8. Ramirez, D. M. and Bentley, W. E. (1993) J. Biotechnol. 71, 39-58
9. Zhu, Y., Rinzema, A., Tramper, J. and Bol, J. (1996) Biotechnol. Bioeng. 50, 291-298
10. Paul, A., Bhosale, S. H., Maity, T. K. and Deshpande, V. V. (1998) Enzyme Microb. Technol. 23, 506-511
11. Han, L., Doverskog, M., Enfors, S. O. and Haggstrom, L. (2002) J. Biotechnol. 92, 237-249
12. Konishi, M., Hano, Y., Takayama, M., Nomura, T., Hamzah, A. S., Ahmad, R. B., Jasmani, H., Yin, L., Jian, C., Mao, Y. Y. et al. (1998) Process Biochem. 33, 709-714
13. Varma, A. and Palsson, B. O. (1994) Nat. Biotechnol. 12, 994-998
14. Xie, L. and Wang, D. I. C. (1994) Biotechnol. Bioeng. 43, 1184-1194
15. Sambrook, J., Fritsch, E. F. and Maniatis, T. (2001) Molecular Cloning: A Laboratory Manual, 3rd edn, Cold Spring Harbor Laboratory Press, Plainview, NY
16. Khalilzadeh, R., Shojaosadati, S. A., Maghsoudi, N., Mohammadian-Mosaabadi, J., Mohammadi, M. R., Bahrami, A., Maleksabet, N., Nassiri-Khalilli, M. A., Ebrahimi, M. and Naderimanesh, H. (2004) J. Ind. Microbiol. Biotechnol. 31, 63-69
17. Babaeipour, V., Shojaosadati, S. A., Robatjazi, S. M., Khalilzadeh, R. and Maghsoudi, N. (2007) Process Biochem. 42, 112-117
18. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254
19. Wang, Z., Le, G., Shi, Y. and Wegrzyn, G. (2001) Process Biochem. 36, 1085-1093
20. Pramanik, J. and Keasling, J. D. (1997) Biotechnol. Bioeng. 56, 398-421
21. Ingraham, J. L., Maaloe, O., Niedhardt, F. C. and Sunderland, M. A. (1983) Growth of Bacterial Cells, p. 267, Sinauer Associates, Sunderland, MA
22. Stouthamer, H., Kumazawa, W. and Motoki, M. (1994) Anton. Leeuw. Int. J. G. 39, 545-565
23. Mathe, K. (1973) Statistical Analysis in Biology, 2nd edn, p. 245, Chapman and Hall, London
24. Kumar, J. K., Tabor, S. and Richardson, C. C. (2004) Proc. Natl. Acad. Sci. U.S.A. 101, 3759-3764
25. Lee, D. H., Kim, S. G., Park, Y. C., Nam, S. W., Lee, K. H. and Seo, J. H. (2007) J. Chromatogr. B 849, 323-330
26. Ow, D. S. W., Nissom, P. M., Philp, R., Oh, S. K. W. and Yap, M. G. S. (2006) Enzyme Microb. Tech. 39, 391-398
27. Edwards, J. S. and Palsson, B. O. (2000) BMC Bioinformatics 1, 27-36
28. Sanden, A. M., Prytz, I., Tubulekas, I., Forberg, C., Le, H., Hektor, A.,Neubauer, P., Pragai, Z., Harwood, C.,Ward, A., et al. (2003) Biotechnol. Bioeng. 81, 158-166
29. Eitman Mark, A. and Altman, E. (2006) Trends Biotechnol. 24, 530-536