Quantitative image analysis of gamma-H2AX foci induced by ionizing radiation applying open source programs

Analytical and Quantitative Cytology and Histology

Volumn 34, Issue 2, 2012, Pages 66-71

  González, Jorge Ernesto ^a   Lee, Manual ^b   Barquinero, Joan Francesc ^c   Valente, Marco ^d   Roch Lefèvre, Sandrine ^d   García , Omar ^a  

^a Center for Radiation Protection and Hygiene   (Cuba)

^b Environmental Regulation and Nuclear Safety   (Cuba)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^d INSTITUT DE RADIOPROTECTION ET DE SÛRETÉ NUCLÉAIRE IRSN   (France)

Author keywords

Gamma H2AX; Image analysis; Ionizing radiation

Indexed keywords

GAMMA H2AX PROTEIN; HISTONE H2AX; UNCLASSIFIED DRUG;

ARTICLE; AUTOMATION; CELL CULTURE; CELL NUCLEUS; CELL STRUCTURE; CELLPROFILER PIPELINE SOFTWARE; COMPUTER PROGRAM; FOCICOUNTER SOFTWARE; HUMAN; HUMAN CELL; IMAGE ANALYSIS; IONIZING RADIATION; PRIORITY JOURNAL; PROTEIN ANALYSIS;

CELL DIVISION; CELL LINE, TUMOR; CELL NUCLEUS; DNA DAMAGE; HISTONES; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; PATHOLOGY, CLINICAL; RADIATION, IONIZING; SKIN NEOPLASMS; SOFTWARE;

EID: 84861001561     PISSN: 08846812     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Rogakou EP, Pilch DR, Orr AH, Ivanova VS, Bonner WM: DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139. J Biol Chem 1998;273:5858-5868
2. Rothkamm K, Balroop S, Shekhdar J, Fernie P, Goh V: Leukocyte DNA damage after multi-detector row CT: A quantitative biomarker of low-level radiation exposure. Radiology 2007;242:244-251
3. Redon CE, Nakamura AJ, Zhang Y-W, Ji J, Bonner WM, Kinders RJ, Parchment RE, Doroshow J H, Pommier Y: Histone γH2AX and poly (ADP-ribose) as clinical pharmacodynamic biomarkers. Clin Cancer Res 2010;16:4532-4542
4. Roch-Lefèvre S, Mandina T, Voisin PA, Gruel G, Gonzalez Mesa JE, Valente M, Bonnesoeur P, Garcia O, Voisin PH, Roy L: Quantification of gamma-H2AX foci in human lymphocytes: An automatic scoring method for biological dosimetry after ionizing radiation exposure. Radiat Res 2011;174:185- 194
5. González JE, Roch-Lefèvre S H, Mandina T, García O, Roy L: Induction of γ-H2AX foci in human exfoliated buccal cells after in vitro exposure to ionizing radiation. Int J Radiat Biol 2010;86:752-759
6. Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, Friman O, Guertin DA, Chang JH, Lindquist RA, Moffat J, Golland P, Sabatini DM: CellProfiler: Image analysis software for identifying and quantifying cell phenotypes. Genome Biol 2006;7:R100
7. Jost G, Golfier S, Pietsch H, Lengsfeld P, Voth M, Schmid TE, Eckardt-Schupp F, Schmid E: The influence of x-ray contrast agents in computed tomography on the induction of dicentrics and ©-H2AX foci in lymphocytes of human blood samples. Phys Med Biol 2009;54:6029-6039
8. Jucha A, Wegierek-Ciuk A, Koza Z, Lisowska H, Wojcik A, Wojewodzka M, Lankoff A: FociCounter: A freely available PC programme for quantitative and qualitative analysis of gamma-H2AX foci. Mutat Res 2010;696:16-20
9. Mah L-J, Orlowski C, Ververis K, Vasireddy RS, El-Osta A, Karagiannis TC: Evaluation of the efficacy of radiationmodifying compounds using gH2AX as a molecular marker of DNA double-strand breaks. Genome Integ 2011;2:3
10. Valente M, Voisin P, Laloi P, Roy L, Roch-Lefèvre S: Automated gamma-H2AX focus scoring method for human lymphocytes after ionizing radiation exposure. Radiat Meas 2011;46:871-876
11. Gundersen HJG, Bendtsen TF, Korbo L,Marcussen N, Møller A, Nielsen K, Nyengaard JR, Pakkenberg B, Sørensen FB, Vesterby A, West MJ: Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 1988;96:379-394
12. Bocker W, Iliakis G. Computational methods for analysis of foci: Validation for radiation-induced gamma-H2AX foci in human cells. Radiat Res 2006;165:113-124
13. Bonner WM, Redon CE, Dickey JS, Nakamura AJ, Sedelnikova OA, Solier S, Pommier Y: © H2AX and cancer. Nat Rev 2008;8:957-967