Cloning and expression of acidstable, high maltose-forming, Ca 2+-independent α-amylase from an acidophile Bacillus acidicola and its applicability in starch hydrolysis

Extremophiles

Volumn 16, Issue 3, 2012, Pages 515-522

  Sharma, Archana ^a   Satyanarayana, T ^a  

^a UNIVERSITY OF DELHI   (India)

Author keywords

Amylase; Acidstable; Bacillus acidicola; Gene cloning

Indexed keywords

AMYLASE; BACTERIAL PROTEIN; CALCIUM; STARCH;

ARTICLE; BACILLUS; BIOSYNTHESIS; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HYDROLYSIS; METABOLISM; MOLECULAR CLONING; PH; PHYSIOLOGY;

ALPHA-AMYLASES; BACILLUS; BACTERIAL PROTEINS; CALCIUM; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; STARCH;

BACILLUS (BACTERIUM); BACILLUS ACIDICOLA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 84860836394     PISSN: 14310651     EISSN: 14334909     Source Type: Journal    
DOI: 10.1007/s00792-012-0451-2     Document Type: Article

References (30)

1. Antranikian G (1992) Microbial degradation of starch. In: Wink-elmann G (ed) Microbial degradation of natural products, vol 2. VCH, Weilheim, pp 28-50.
2. Arikan B (2008) Highly thermostable, thermophilic, alkaline, SDS and chelator resistant amylase from a thermophilic Bacillus sp. isolate A3-15. Biores Technol 99: 3071-3076.
3. Asgher M, Javaid AM, Rahman SU, Legge RL (2007) A thermostable α-amylase from a moderately thermophilic Bacillus subtilis strain for starch processing. J Food Eng 79: 950-955.
4. Asoodeh A, Chamani J, Lagzian M (2010) A novel thermostable, acidophilic α-amylase from a new thermophilic "Bacillus sp. Ferdowsicous" isolated from Ferdows hot mineral spring in Iran: purification and biochemical characterization. Inter J Biol Macromol 46: 289-297.
5. Bazzicalupo M, Fani R (1995) The use of RAPD for generating specific DNA probes for microorganisms. In: Clapp JP (ed) Methods in molecular biology, S-species diagnostic protocols: PCR and other nucleic acids methods, vol 50. Humana Press Inc., Totowa, pp 155-177.
6. Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman G (2003) Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Proc Biochem 38: 1397-1403.
7. Chauthaiwale J, Rao M (1994) Chemical modification of xylanase from alkalothermophilic Bacillus species: evidence for essential carboxyl group. Biochim Biophys Acta 1204: 164-168.
8. Dey G, Palit S, Banerjee R, Maiti BR (2002) Purification and characterization of maltooligosaccharide-forming amylase from Bacillus circulans GRS 313. J Ind Microbiol Biotechnol 28: 193-200.
9. Gupta R, Gigras P, Mohapatra H, Goswami VK, Chauhan B (2003) Microbial α-amylases: a biotechnological perspective. Proc Biochem 38: 1599-1616.
10. Hansen SA (1975) Thin layer chromatographic method for identification of oligosaccharides in starch hydrolysates. J Chromatography 105: 388-390.
11. Hashim SO, Delgado OD, Martinez MA, Kaul RH, Mulaa FJ, Mattiasson B (2005) Alkaline active maltohexaose-forming α-amylase from Bacillus halodurans LBK 34. Enzym Microb Technol 36: 139-146.
12. Hmidet N, Bayoudh A, Berrin JG, Kanoun S, Juge N, Nasri M (2008) Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1 Cloning, nucleotide sequence and expression of amyN gene in Escherichia coli. Proc Biochem 43: 499-510.
13. Khemakhem B, Ali MB, Aghajari N, Juy M, Haser R, Bejar S (2009) Engineering of the α-amylase from Geobacillus stearothermophilus US100 for detergent incorporation. Biotechnol Bioeng 102: 380-390.
14. Kim J, Nanmori T, Shninke R (1989) Thermostable raw starch-digesting-amylase from Bacillus stearothermophilus. Appl Environ Microbiol 55: 1638-1639.
15. Komissarov AA, Romanova DV, Debabov VG (1995) Complete inactivation of Escherichia coli uridine phosphorylase by modification of Asp with Woodward's reagent K. J Biol Chem 270: 10050-10055.
16. Kumar V, Satyanarayana T (2011) Applicability of thermo-alkali-stable and cellulase-free xylanase from a novel thermo-halo-alkaliphilic Bacillus halodurans in producing xylooligosaccharides. Biotechnol Lett 33: 2279-2285.
17. Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 277: 680-685.
18. Liu W, Shi P, Chen Q, Yang P, Wang G, Wang Y, Luo H, Yao B (2010) Gene cloning, overexpression, and characterization of a xylanase from Penicillium sp. CGMCC 1699. Appl Biochem Biotechnol 162: 1-12.
19. Liu XD, Xu Y (2008) A novel raw starch digesting α-amylase from a newly isolated Bacillus sp. YX-1: purification and characterization. Biores Technol 99: 4315-4320.
20. Lo H-F, Lin Long L, Li C-C, Hsu W-H, Chang C-T (2001) The N-terminal signal sequence and the last 98 amino acids are not essential for the secretion of Bacillus sp. TS-23 α-amylase in Escherichia coli. Curr Microbiol 43: 170-175.
21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193: 265-275.
22. Martin ML, Zeleznak KJ, Hoseney RC (1991) A mechanism for bread firming. I: role of starch hydrolyzing enzymes. Cereal Chem 68: 498-503.
23. Martin ML, Hoseney RC (1991) A mechanism of bread firming. II: role of starch hydrolyzing enzymes. Cereal Chem 68: 503-507.
24. Paoli P, Fiaschi T, Cirri P, Camici G, Manao G, Cappugi G, Raugei G, Moneti G, Ramponi G (1997) Mechanism of acylphosphatase inactivation by Woodward's reagent K. Biochem J 328: 855-861.
25. Rao JLUM, Satyanarayana T (2007) Purification and characterization of a hyperthermostable and high maltogenic α-amylase of an extreme thermophile Geobacillus thermoleovorans. Appl Biochem Biotechnol 142: 179-193.
26. Sajedi RH, Naderi-Mahesh H, Khajeh K, Ahmadvand R, Ranjbar BA, Asoodeh A, Moradian F (2005) A calcium independent α-amylase that is active and stable at low pH from the Bacillus sp. KR-8104. Enzym Microb Technol 36: 666-671.
27. Saxena RK, Dutt K, Agarwal L, Nayyar P (2007) A highly thermostable and alkaline amylase from a Bacillus sp. PN5. Biores Technol 98: 260-265.
28. 2+-independent and acid stable α-amylase from a novel acidophilic bacterium Bacillus acidicola TSAS1. Biotechnol Lett 32: 1503-1507.
29. 2+-independent α-amylase by Bacillus acidicola. J Biosci Bioeng 111: 550-553.
30. Zhang GM, Huang J, Huang GR, Ma LX, Zhang XE (2007) Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina. Appl Microbiol Biotechnol 74: 339-346.