Molecular cloning and heterologous expression of a new xylanase gene from Plectosphaerella cucumerina

Applied Microbiology and Biotechnology

Volumn 74, Issue 2, 2007, Pages 339-346

  Zhang, Gui Min ^a,c   Huang, Jun ^a   Huang, Guang Rui ^a   Ma, Li Xin ^a   Zhang, Xian En ^b  

^a HUBEI UNIVERSITY   (China)

^b WUHAN INSTITUTE OF VIROLOGY   (China)

^c HUAZHONG AGRICULTURAL UNIVERSITY   (China)

Author keywords

Genome walking PCR; Heterologous expression; Pichia pastoris; Plectosphaerella cucumerina; Xylanase

Indexed keywords

CLONING; DNA SEQUENCES; ENZYME KINETICS; GENES; GENETIC ENGINEERING; PH EFFECTS;

GENOME-WALKING PCR; HETEROLOGOUS EXPRESSION; PICHIA PASTORIS; PLECTOSPHAERELLA CUCUMERINA; XYLANASE;

ENZYMES;

EDETIC ACID; XYLAN ENDO 1,3 BETA XYLOSIDASE;

EDTA; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ENGINEERING; INHIBITION; POLYMERASE CHAIN REACTION; POLYSACCHARIDE; REACTION KINETICS; YEAST;

ARTICLE; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME PURIFICATION; ENZYME STABILITY; FUNGAL GENE; FUNGAL STRAIN; FUNGUS CULTURE; GENOME ANALYSIS; HETEROLOGOUS EXPRESSION; INTRON; MOLECULAR CLONING; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH MEASUREMENT; PICHIA PASTORIS; PLECTOSPHAERELLA CUCUMERINA; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; ROOM TEMPERATURE; SEQUENCE ANALYSIS; SUPERNATANT; TEMPERATURE DEPENDENCE;

BIOTECHNOLOGY; CHROMOSOME WALKING; CLONING, MOLECULAR; ENDO-1,4-BETA XYLANASES; ENZYME STABILITY; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; PHYLLACHORALES; PICHIA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

FUNGI; PICHIA PASTORIS; PLECTOSPHAERELLA CUCUMERINA;

EID: 33846845195     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0648-3     Document Type: Article

References (30)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389-3402
2. Apel PC, Panaccione DG, Holden FR, Walton JD (1993) Cloning and targeted gene disruption of XYL1, a beta 1,4-xylanase gene from the maize pathogen Cochliobolus carbonum. Mol Plant Microbe Interact 6:467-473
3. Araki T, Tani S, Maeda K, Hashikawa S, Nakagawa H, Morishita T (1999) Purification and characterization of beta-1, 3-xylanase from a marine bacterium, Vibrio sp. XY-214. Biosci Biotechnol Biochem 63:2017-2019
4. Atkins SD, Clark IM, Sosnowska D, Hirsch PR, Kerry BR (2003) Detection and quantification of Plectosphaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selectRive media, and baiting. Appl Environ Microbiol 69:4788-4793
5. Badal CS (2002) Production, purification and properties of xylanase from a newly isolated Fusarium proliferatum. Process Biochem 37:1279-1284
6. Biswas MJ, Jana SC, Mishra AK, Nanda G (1990) Production, purification and characterization of xylanase from hyperxylanolytic mutant of Aspergillus ochraceus. Biotechnol Bioeng 35:244-251
7. Chandrakant P, Bisaria VS (1998) Simultaneous bioconversion of cellulose and hemicellulose to ethanol. Crit Rev Biotechnol 18:295-331
8. Degefu Y, Paulin L, Lubeck PS (2001) Cloning, sequencing and expression of a xylanase gene from the maize pathogen Helminthosporium turcicum Pass. Eur J Plant Pathol 107:457-465
9. Fialho MB, Carmona EC (2004) Purification and characterization of xylanase from Aspergillus giganteus. Folia Microbiol 49:13-18
10. Gorbacheva IV, Rodionova NA (1997) Studies on xylan degrading enzymes. I. Purification and characterization of endo-1,4-β-xylanase from Aspergillus niger str. 14. Biochim Biophys Acta 484:79-93
11. Gupta S, Bhushan B, Hoondal GS (2000) Isolation, purification and characterization of xylanase from Staphylococcus sp. SG-13 and its application in biobleaching of kraft pulp. J Appl Microbiol 88:325-334
12. Heinz P, Walter S (1995) Induction of endo-xylanase in the fungus Thermomyces lanuginosus. Enzyme Microb Technol 17:114-118
13. Henrissat B, Teeri TT, Warren RAJ (1998) A scheme for designating enzymes that hydrolyse the polysaccharides in the cell walls of plants. FEBS Lett 425:352-354
14. Huang YH, Long ZE, Li SZ, Pei LL, Wang SX, Yu M (2002) Isolation and identification of prolific microorganism in xylanase. Journal of Jiangxi Normal University (Natural science ed.) (Chinese) 26:245-248
15. Iefuji H, Chino M, Kato M, Iimura Y, (1996) Acid xylanase from yeast Cryptococcus sp. S-2: purification, characterization, cloning, and sequencing. Biosci Biotechnol Biochem 60:1331-1338
16. Irena R, Jacek P, Stanislaw B (2006) Isolation and properties of Aspergillus niger IBT-90 xylanase for bakery. Appl Microbiol Biotechnol 69:665-671
17. Jeffrey AR, Garret BN, Katrina LH (1991) DNA isolation from small tissue samples using salt and spermine. Nucleic Acids Res 19:6053
18. John M, Schmidt B, Schmidt J (1979) Purification and some properties of five endo-1,4-β-xylanases and β-xylosidases produced by strain of A. niger. Can J Biochem 157:125-134
19. Krisana A, Rutchadaporn S, Jarupan G, Lily E, Sutipa T, Kanyawim K (2005) Endo-1,4-beta-xylanase B from Aspergillus cf. niger BCC14405 isolated in Thailand: purification, characterization and gene isolation. J Biochem Mol Biol 38:17-23
20. Liu R, Qu Y, Jiang Y, Gao P (1999) Purification and characterization of alkaline xylanases from Pseudomonas G6-2. Wei Sheng Wu Xue Bao 39:132-136
21. Marais JP, De Wit JL, Quicke GV (1966) A critical examination of the Nelson-Somogyi method for the determination of reducing sugars. Anal Biochem 15:373-381
22. Miller GL, Blum R, Glennon WE, Burton AL (1960) Measurement of carboxymethyl cellulase activity. Anal Biochem 2:127-132
23. Morris DD, Gibbs MD, Chin CW, Koh MH, Wong KK, Allison RW, Nelson PJ, Bergquist PL (1998) Cloning of the xynB gene from Dictyoglomus thermophilum Rt46B.1 and action of the gene product on kraft pulp. Appl Environ Microbiol 64:1759-1765
24. Morris DD, Gibbs MD, Ford M, Thomas J, Bergquist PL (1999) Family 10 and 11 xylanase genes from Caldicellulosiruptor sp. Rt69B.1. Extremophiles 3:103-111
25. Neeta K, Abhay S, Mala R (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiol Rev 23:411-456
26. Polizeli ML, Rizzatti AC, Monti R, Terenzi HF, Jorge JA, Amorim DS (2005) Xylanases from fungi: properties and industrial applications. Appl Microbiol Biotechnol 67:577-591
27. Rogalski J, Oleszek M, Tokarzewska-Zadora J (2001) Purification and characterization of two endo-1,4-β-xylanases and β-xylosidases from Phelbia radiata. Acta Microbiol Pol 50:117-128
28. Sapag A, Wouters J, Lambert C, de Ioannes P, Eyzaguirre J, Depiereux E (2002) The endoxylanases from family 11: computer analysis of protein sequences reveals important structural and phylogenetic relationships. J Biotechnol 95:109-131
29. Schlacher A, Holzmann K, Hayn M, Steiner W, Schwab H (1996) Cloning and characterization of the gene for the thermostable xylanase XynA from Thermomyces lanuginosus. J Biotechnol 49:211-218
30. Yamaura I, Koga T, Matsumoto T, Kato T (1997) Purification and some properties of endo-1,4-beta-D-xylanase from a fresh-water mollusc, Pomacea insularus (de Ordigny). Biosci Biotechnol Biochem 61:615-620