Small RNAs in streptococci

RNA Biology

Volumn 9, Issue 4, 2012, Pages 414-426

  Le Rhun, Anaïs ^a   Charpentier, Emmanuelle ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

Adaptation; Bacterial immunity; CRISPR Cas; Gene regulation; Pathogenicity; Small RNAs; Streptococci; Trans acting RNAs; Virulence

Indexed keywords

BACTERIAL RNA; CASPASE 2; CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN; FIBRINOGEN; GLYCOSIDASE; HISTIDINE; M PROTEIN; MESSENGER RNA; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; STREPTOKINASE; STREPTOLYSIN S; TRANSFER RNA; VIRULENCE FACTOR;

ANAEROBIC CAPACITY; BACTERIAL VIRULENCE; CELL COMMUNICATION; DEFENSE MECHANISM; DISEASE COURSE; ENVIRONMENTAL CHANGE; FIRMICUTES; GENE EXPRESSION; GENE TRANSFER; HUMAN; INTEGRATION; LACTOBACILLALES; MICROARRAY ANALYSIS; MOUTH MUCOSA; NONHUMAN; PROTEIN INTERACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; RNA SEQUENCE; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS; STREPTOCOCCUS AGALACTIAE; STREPTOCOCCUS DYSGALACTIAE; STREPTOCOCCUS MITIS; STREPTOCOCCUS MUTANS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PYOGENES; STREPTOCOCCUS SANGUINIS; STREPTOCOCCUS THERMOPHILUS; TRANSLATION INITIATION; UROGENITAL SYSTEM;

BACTERIA (MICROORGANISMS);

EID: 84860733797     PISSN: 15476286     EISSN: 15558584     Source Type: Journal    
DOI: 10.4161/rna.20104     Document Type: Review

References (106)

1. Romby P, Charpentier E. An overview of RNAs with regulatory functions in gram-positive bacteria. Cell Mol Life Sci 2010; 67:217-37.
2. Storz G, Vogel J, Wassarman KM. Regulation by small RNAs in bacteria: expanding frontiers. Mol Cell 2011; 43:880-91.
3. Waters LS, Storz G. Regulatory RNAs in bacteria. Cell 2009; 136:615-28.
4. Winkler WC, Breaker RR. Regulation of bacterial gene expression by riboswitches. Annu Rev Microbiol 2005; 59:487-517. (Pubitemid 41507440)
5. Zhang J, Lau MW, Ferre-D'Amare AR. Ribozymes and riboswitches: modulation of RNA function by small molecules. Biochemistry 2010; 49:9123-31.
6. Irnov, Kertsburg A, Winkler WC. Genetic control by cis-acting regulatory RNAs in Bacillus subtilis: general principles and prospects for discovery. Cold Spring Harb Symp Quant Biol 2006; 71:239-49.
7. Breaker RR. Prospects for riboswitch discovery and analysis. Mol Cell 2011; 43:867-79.
8. Wadler CS, Vanderpool CK. A dual function for a bacterial small RNA: SgrS performs base pairing-dependent regulation and encodes a functional polypeptide. Proc Natl Acad Sci USA 2007; 104:20454-9.
9. Novick RP, Ross HF, Projan SJ, Kornblum J, Kreiswirth B, Moghazeh S. Synthesis of staphylococcal virulence factors is controlled by a regulatory RNA molecule. EMBO J 1993; 12:3967-75. (Pubitemid 23282772)
10. Vanderpool CK, Balasubramanian D, Lloyd CR. Dualfunction RNA regulators in bacteria. Biochimie 2011; 93:1943-9.
11. Brantl S. Regulatory mechanisms employed by cisencoded antisense RNAs. Curr Opin Microbiol 2007; 10:102-9. (Pubitemid 46590052)
12. Wagner EG, Altuvia S, Romby P. Antisense RNAs in bacteria and their genetic elements. Adv Genet 2002; 46:361-98.
13. Toledo-Arana A, Repoila F, Cossart P. Small noncoding RNAs controlling pathogenesis. Curr Opin Microbiol 2007; 10:182-8. (Pubitemid 46590044)
14. Romby P, Vandenesch F, Wagner EG. The role of RNAs in the regulation of virulence-gene expression. Curr Opin Microbiol 2006; 9:229-36.
15. Vogel J, Luisi BF. Hfq and its constellation of RNA. Nat Rev Microbiol 2011; 9:578-89.
16. Felden B, Vandenesch F, Bouloc P, Romby P. The Staphylococcus aureus RNome and its commitment to virulence. PLoS Pathog 2011; 7:1002006.
17. Mraheil MA, Billion A, Kuenne C, Pischimarov J, Kreikemeyer B, Engelmann S, et al. Comparative genome-wide analysis of small RNAs of major Grampositive pathogens: from identification to application. Microb Biotechnol 2010; 3:658-76.
18. Ramirez-Pena E, Trevino J, Liu Z, Perez N, Sumby P. The group A Streptococcus small regulatory RNA FasX enhances streptokinase activity by increasing the stability of the ska mRNA transcript. Mol Microbiol 2010; 78:1332-47.
19. Deltcheva E, Chylinski K, Sharma CM, Gonzales K, Chao Y, Pirzada ZA, et al. CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III. Nature 2011; 471:602-7.
20. Garneau JE, Dupuis ME, Villion M, Romero DA, Barrangou R, Boyaval P, et al. The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA. Nature 2010; 468:67-71.
21. Pichon C, du Merle L, Caliot ME, Trieu-Cuot P, Le Bouguenec C. An in silico model for identification of small RNAs in whole bacterial genomes: characterization of antisense RNAs in pathogenic Escherichia coli and Streptococcus agalactiae strains. Nucleic Acids Res 2011; Dec 1. [Epub ahead of print].
22. Hardie JM, Whiley RA. Classification and overview of the genera Streptococcus and Enterococcus. Soc Appl Bacteriol Symp Ser 1997; 26:1-11.
23. Facklam R. What happened to the streptococci: overview of taxonomic and nomenclature changes. Clin Microbiol Rev 2002; 15:613-30. (Pubitemid 41184081)
24. Cunningham MW. Pathogenesis of group A streptococcal infections. Clin Microbiol Rev 2000; 13:470-511. (Pubitemid 30452618)
25. Cunningham MW. Pathogenesis of group A streptococcal infections and their sequelae. Adv Exp Med Biol 2008; 609:29-42.
26. Reinert RR. The antimicrobial resistance profile of Streptococcus pneumoniae. Clin Microbiol Infect 2009; 15:7-11.
27. Charpentier E, Tuomanen E. Mechanisms of antibiotic resistance and tolerance in Streptococcus pneumoniae. Microbes Infect 2000; 2:1855-64. (Pubitemid 32063539)
28. Feldman C, Anderson R. New insights into pneumococcal disease. Respirology 2009; 14:167-79.
29. Berardi A, Tzialla C, Riva M, Cerbo RM, Creti R. Group B streptococcus: early- and late-onset infections. J Chemother 2007; 19:24-7.
30. Maisey HC, Doran KS, Nizet V. Recent advances in understanding the molecular basis of group B Streptococcus virulence. Expert Rev Mol Med 2008; 10:27.
31. Hava DL, LeMieux J, Camilli A. From nose to lung: the regulation behind Streptococcus pneumoniae virulence factors. Mol Microbiol 2003; 50:1103-10. (Pubitemid 37475846)
32. Kreikemeyer B, McIver KS, Podbielski A. Virulence factor regulation and regulatory networks in Streptococcus pyogenes and their impact on pathogen-host interactions. Trends Microbiol 2003; 11:224-32. (Pubitemid 36645006)
33. Livny J, Brencic A, Lory S, Waldor MK. Identification of 17 Pseudomonas aeruginosa sRNAs and prediction of sRNA-encoding genes in 10 diverse pathogens using the bioinformatic tool sRNAPredict2. Nucleic Acids Res 2006; 34:3484-93.
34. Tsui HC, Mukherjee D, Ray VA, Sham LT, Feig AL, Winkler ME. Identification and characterization of noncoding small RNAs in Streptococcus pneumoniae serotype 2 strain D39. J Bacteriol 2010; 192:264-79.
35. Livny J, Teonadi H, Livny M, Waldor MK. Highthroughput, kingdom-wide prediction and annotation of bacterial non-coding RNAs. PLoS ONE 2008; 3:3197.
36. Raasch P, Schmitz U, Patenge N, Vera J, Kreikemeyer B, Wolkenhauer O. Non-coding RNA detection methods combined to improve usability, reproducibility and precision. BMC Bioinformatics 2010; 11:491.
37. Halfmann A, Kovacs M, Hakenbeck R, Bruckner R. Identification of the genes directly controlled by the response regulator CiaR in Streptococcus pneumoniae: five out of 15 promoters drive expression of small noncoding RNAs. Mol Microbiol 2007; 66:110-26. (Pubitemid 47414793)
38. Perez N, Trevino J, Liu Z, Ho SC, Babitzke P, Sumby P. A genome-wide analysis of small regulatory RNAs in the human pathogen group A Streptococcus. PLoS One 2009; 4:7668.
39. Kumar R, Shah P, Swiatlo E, Burgess SC, Lawrence ML, Nanduri B. Identification of novel non-coding small RNAs from Streptococcus pneumoniae TIGR4 using high-resolution genome tiling arrays. BMC Genomics 2010; 11:350.
40. Lee HJ, Hong SH. Analysis of microRNA-size, small RNAs in Streptococcus mutans by deep sequencing. FEMS Microbiol Lett 2012; 326:131-6.
41. Acebo P, Martin-Galiano AJ, Navarro S, Zaballos A, Amblar M. Identification of 88 regulatory small RNAs in the TIGR4 strain of the human pathogen Streptococcus pneumoniae. RNA 2012; 18:530-46.
42. Kreikemeyer B, Boyle MD, Buttaro BA, Heinemann M, Podbielski A. Group A streptococcal growth phase-associated virulence factor regulation by a novel operon (Fas) with homologies to two-component-type regulators requires a small RNA molecule. Mol Microbiol 2001; 39:392-406. (Pubitemid 32113511)
43. Mangold M, Siller M, Roppenser B, Vlaminckx BJ, Penfound TA, Klein R, et al. Synthesis of group A streptococcal virulence factors is controlled by a regulatory RNA molecule. Mol Microbiol 2004; 53:1515-27. (Pubitemid 39209121)
44. Roberts SA, Scott JR. RivR and the small RNA RivX: the missing links between the CovR regulatory cascade and the Mga regulon. Mol Microbiol 2007; 66:1506- 22.
45. Trevino J, Perez N, Sumby P. The 4.5S RNA component of the signal recognition particle is required for group A Streptococcus virulence. Microbiology 2010; 156:1342-50.
46. Steiner K, Malke H. relA-Independent amino acid starvation response network of Streptococcus pyogenes. J Bacteriol 2001; 183:7354-64. (Pubitemid 33121869)
47. Siller M, Janapatla RP, Pirzada ZA, Hassler C, Zinkl D, Charpentier E. Functional analysis of the group A streptococcal luxS/AI-2 system in metabolism, adaptation to stress and interaction with host cells. BMC Microbiol 2008; 8:188.
48. Klenk M, Koczan D, Guthke R, Nakata M, Thiesen HJ, Podbielski A, et al. Global epithelial cell transcriptional responses reveal Streptococcus pyogenes Fas regulator activity association with bacterial aggressiveness. Cell Microbiol 2005; 7:1237-50. (Pubitemid 41179417)
49. Steiner K, Malke H. Dual control of streptokinase and streptolysin S production by the covRS and fasCAX two-component regulators in Streptococcus dysgalactiae subsp. equisimilis. Infect Immun 2002; 70:3627-36. (Pubitemid 34666001)
50. Podkaminski D, Vogel J. Small RNAs promote mRNA stability to activate the synthesis of virulence factors. Mol Microbiol 2010; 78:1327-31.
51. Datta V, Myskowski SM, Kwinn LA, Chiem DN, Varki N, Kansal RG, et al. Mutational analysis of the group A streptococcal operon encoding streptolysin S and its virulence role in invasive infection. Mol Microbiol 2005; 56:681-95. (Pubitemid 40562362)
52. Liu S, Sela S, Cohen G, Jadoun J, Cheung A, Ofek I. Insertional inactivation of streptolysin S expression is associated with altered riboflavin metabolism in Streptococcus pyogenes. Microb Pathog 1997; 22:227-34. (Pubitemid 27187466)
53. Li Z, Sledjeski DD, Kreikemeyer B, Podbielski A, Boyle MD. Identification of pel, a Streptococcus pyogenes locus that affects both surface and secreted proteins. J Bacteriol 1999; 181:6019-27. (Pubitemid 29459912)
54. Biswas I, Germon P, McDade K, Scott JR. Generation and surface localization of intact M protein in Streptococcus pyogenes are dependent on sagA. Infect Immun 2001; 69:7029-38. (Pubitemid 32995463)
55. Betschel SD, Borgia SM, Barg NL, Low DE, De Azavedo JC. Reduced virulence of group A streptococcal Tn916 mutants that do not produce streptolysin S. Infect Immun 1998; 66:1671-9. (Pubitemid 128471534)
56. Malke H, Steiner K, McShan WM, Ferretti JJ. Linking the nutritional status of Streptococcus pyogenes to alteration of transcriptional gene expression: the action of CodY and RelA. Int J Med Microbiol 2006; 296:259-75. (Pubitemid 44016160)
57. Shelburne SA, 3rd, Sumby P, Sitkiewicz I, Granville C, DeLeo FR, Musser JM. Central role of a bacterial two-component gene regulatory system of previously unknown function in pathogen persistence in human saliva. Proc Natl Acad Sci USA 2005; 102:16037-42. (Pubitemid 41552863)
58. Voyich JM, Braughton KR, Sturdevant DE, Vuong C, Kobayashi SD, Porcella SF, et al. Engagement of the pathogen survival response used by group A Streptococcus to avert destruction by innate host defense. J Immunol 2004; 173:1194-201. (Pubitemid 38924296)
59. Salim KY, de Azavedo JC, Bast DJ, Cvitkovitch DG. Role for sagA and siaA in quorum sensing and iron regulation in Streptococcus pyogenes. Infect Immun 2007; 75:5011-7. (Pubitemid 47502174)
60. Beckert S, Kreikemeyer B, Podbielski A. Group A streptococcal rofA gene is involved in the control of several virulence genes and eukaryotic cell attachment and internalization. Infect Immun 2001; 69:534-7. (Pubitemid 32038354)
61. Chaussee MS, Sylva GL, Sturdevant DE, Smoot LM, Graham MR, Watson RO, et al. Rgg influences the expression of multiple regulatory loci to coregulate virulence factor expression in Streptococcus pyogenes. Infect Immun 2002; 70:762-70. (Pubitemid 34083838)
62. Molinari G, Rohde M, Talay SR, Chhatwal GS, Beckert S, Podbielski A. The role played by the group A streptococcal negative regulator Nra on bacterial interactions with epithelial cells. Mol Microbiol 2001; 40:99-114. (Pubitemid 32322865)
63. Gao J, Gusa AA, Scott JR, Churchward G. Binding of the global response regulator protein CovR to the sag promoter of Streptococcus pyogenes reveals a new mode of CovR-DNA interaction. J Biol Chem 2005; 280:38948-56. (Pubitemid 41713844)
64. Kwinn LA, Khosravi A, Aziz RK, Timmer AM, Doran KS, Kotb M, et al. Genetic characterization and virulence role of the RALP3/LSA locus upstream of the streptolysin S operon in invasive M1T1 Group A Streptococcus. J Bacteriol 2007; 189:1322-9. (Pubitemid 46239753)
65. Lyon WR, Madden JC, Levin JC, Stein JL, Caparon MG. Mutation of luxS affects growth and virulence factor expression in Streptococcus pyogenes. Mol Microbiol 2001; 42:145-57. (Pubitemid 34212168)
66. Kihlberg BM, Cooney J, Caparon MG, Olsen A, Bjorck L. Biological properties of a Streptococcus pyogenes mutant generated by Tn916 insertion in mga. Microb Pathog 1995; 19:299-315. (Pubitemid 26022897)
67. Kinkel TL, McIver KS. CcpA-mediated repression of streptolysin S expression and virulence in the group A streptococcus. Infect Immun 2008; 76:3451-63.
68. Heath A, DiRita VJ, Barg NL, Engleberg NC. A two-component regulatory system, CsrR-CsrS, represses expression of three Streptococcus pyogenes virulence factors, hyaluronic acid capsule, streptolysin S and pyrogenic exotoxin B. Infect Immun 1999; 67:5298-305. (Pubitemid 29455305)
69. Federle MJ, McIver KS, Scott JR. A response regulator that represses transcription of several virulence operons in the group A streptococcus. J Bacteriol 1999; 181:3649-57. (Pubitemid 29283790)
70. Kreikemeyer B, Nakata M, Koller T, Hildisch H, Kourakos V, Standar K, et al. The Streptococcus pyogenes serotype M49 Nra-Ralp3 transcriptional regulatory network and its control of virulence factor expression from the novel eno ralp3 epf sagA pathogenicity region. Infect Immun 2007; 75:5698-710. (Pubitemid 350207806)
71. Shelburne SA, Olsen RJ, Suber B, Sahasrabhojane P, Sumby P, Brennan RG, et al. A combination of independent transcriptional regulators shapes bacterial virulence gene expression during infection. PLoS Pathog 2010; 6:1000817.
72. Nizet V. Streptococcal beta-hemolysins: genetics and role in disease pathogenesis. Trends Microbiol 2002; 10:575-80. (Pubitemid 36139879)
73. Roberts SA, Churchward GG, Scott JR. Unraveling the regulatory network in Streptococcus pyogenes: the global response regulator CovR represses rivR directly. J Bacteriol 2007; 189:1459-63. (Pubitemid 46239768)
74. Marx P, Nuhn M, Kovacs M, Hakenbeck R, Bruckner R. Identification of genes for small non-coding RNAs that belong to the regulon of the two-component regulatory system CiaRH in Streptococcus. BMC Genomics 2010; 11:661.
75. Rosch JW, Vega LA, Beyer JM, Lin A, Caparon MG. The signal recognition particle pathway is required for virulence in Streptococcus pyogenes. Infect Immun 2008; 76:2612-9.
76. Hasona A, Crowley PJ, Levesque CM, Mair RW, Cvitkovitch DG, Bleiweis AS, et al. Streptococcal viability and diminished stress tolerance in mutants lacking the signal recognition particle pathway or YidC2. Proc Natl Acad Sci USA 2005; 102:17466-71. (Pubitemid 41740889)
77. Hasona A, Zuobi-Hasona K, Crowley PJ, Abranches J, Ruelf MA, Bleiweis AS, et al. Membrane composition changes and physiological adaptation by Streptococcus mutans signal recognition particle pathway mutants. J Bacteriol 2007; 189:1219-30. (Pubitemid 46239743)
78. Deveau H, Garneau JE, Moineau S. CRISPR/Cas system and its role in phage-bacteria interactions. Annu Rev Microbiol 2010; 64:475-93.
79. van der Oost J, Brouns SJ. RNAi: prokaryotes get in on the act. Cell 2009; 139:863-5.
80. Makarova KS, Haft DH, Barrangou R, Brouns SJ, Charpentier E, Horvath P, et al. Evolution and classification of the CRISPR-Cas systems. Nat Rev Microbiol 2011; 9:467-77.
81. Horvath P, Barrangou R. CRISPR/Cas, the immune system of bacteria and archaea. Science 2010; 327:167-70.
82. Marraffini LA, Sontheimer EJ. CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea. Nat Rev Genet 2010; 11:181-90.
83. Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol 1987; 169:5429-33. (Pubitemid 18017837)
84. Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, et al. CRISPR provides acquired resistance against viruses in prokaryotes. Science 2007; 315:1709-12. (Pubitemid 46515624)
85. Deveau H, Barrangou R, Garneau JE, Labonte J, Fremaux C, Boyaval P, et al. Phage response to CRISPR-encoded resistance in Streptococcus thermophilus. J Bacteriol 2008; 190:1390-400. (Pubitemid 351215022)
86. Vojtek I, Pirzada ZA, Henriques-Normark B, Mastny M, Janapatla RP, Charpentier E. Lysogenic transfer of group A Streptococcus superantigen gene among streptococci. J Infect Dis 2008; 197:225-34. (Pubitemid 351263441)
87. Brussow H, Canchaya C, Hardt WD. Phages and the evolution of bacterial pathogens: from genomic rearrangements to lysogenic conversion. Microbiol Mol Biol Rev 2004; 68:560-602. (Pubitemid 39238283)
88. Bolotin A, Quinquis B, Sorokin A, Ehrlich SD. Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin. Microbiology 2005; 151:2551-61. (Pubitemid 41149778)
89. Chattoraj P, Banerjee A, Biswas S, Biswas I. ClpP of Streptococcus mutans differentially regulates expression of genomic islands, mutacin production and antibiotic tolerance. J Bacteriol 2010; 192:1312-23.
90. Horvath P, Coute-Monvoisin AC, Romero DA, Boyaval P, Fremaux C, Barrangou R. Comparative analysis of CRISPR loci in lactic acid bacteria genomes. Int J Food Microbiol 2009; 131:62-70.
91. Horvath P, Romero DA, Coute-Monvoisin AC, Richards M, Deveau H, Moineau S, et al. Diversity, activity and evolution of CRISPR loci in Streptococcus thermophilus. J Bacteriol 2008; 190:1401-12. (Pubitemid 351215023)
92. Maeda Y, Goldsmith CE, Coulter WA, Mason C, Dooley JS, Lowery CJ, et al. Prevalence of clustered regulatory interspaced short palindromic repeat (CRISPR)-like sequences in mitis-group streptococci. Br J Biomed Sci 2011; 68:65-8.
93. Maruyama F, Kobata M, Kurokawa K, Nishida K, Sakurai A, Nakano K, et al. Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content. BMC Genomics 2009; 10:358.
94. Mills S, Griffin C, Coffey A, Meijer WC, Hafkamp B, Ross RP. CRISPR analysis of bacteriophage-insensitive mutants (BIMs) of industrial Streptococcus thermophilus - implications for starter design. J Appl Microbiol 2010; 108:945-55.
95. Mojica FJ, Diez-Villasenor C, Garcia-Martinez J, Almendros C. Short motif sequences determine the targets of the prokaryotic CRISPR defence system. Microbiology 2009; 155:733-40.
96. Mojica FJ, Diez-Villasenor C, Garcia-Martinez J, Soria E. Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements. J Mol Evol 2005; 60:174-82. (Pubitemid 40568857)
97. Nozawa T, Furukawa N, Aikawa C, Watanabe T, Haobam B, Kurokawa K, et al. CRISPR inhibition of prophage acquisition in Streptococcus pyogenes. PLoS One 2011; 6:19543.
98. Pride DT, Sun CL, Salzman J, Rao N, Loomer P, Armitage GC, et al. Analysis of streptococcal CRISPRs from human saliva reveals substantial sequence diversity within and between subjects over time. Genome Res 2011; 21:126-36.
99. Shimomura Y, Okumura K, Murayama SY, Yagi J, Ubukata K, Kirikae T, et al. Complete genome sequencing and analysis of a Lancefield group G Streptococcus dysgalactiae subsp equisimilis strain causing streptococcal toxic shock syndrome (STSS). BMC Genomics 2011; 12:17.
100. van der Ploeg JR. Analysis of CRISPR in Streptococcus mutans suggests frequent occurrence of acquired immunity against infection by M102-like bacteriophages. Microbiology 2009; 155:1966-76.
101. Sapranauskas R, Gasiunas G, Fremaux C, Barrangou R, Horvath P, Siksnys V. The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli. Nucleic Acids Res 2011; 39:9275-82.
102. Terns MP, Terns RM. CRISPR-based adaptive immune systems. Curr Opin Microbiol 2011; 14:321-7.
103. Makarova KS, Aravind L, Wolf YI, Koonin EV. Unification of Cas protein families and a simple scenario for the origin and evolution of CRISPR-Cas systems. Biol Direct 2011; 6:38.
104. Chao Y, Vogel J. The role of Hfq in bacterial pathogens. Curr Opin Microbiol 2010; 13:24-33.
105. Belasco JG. All things must pass: contrasts and commonalities in eukaryotic and bacterial mRNA decay. Nat Rev Mol Cell Biol 2010; 11:467-78.
106. Condon C, Bechhofer DH. Regulated RNA stability in the Gram positives. Curr Opin Microbiol 2011; 14:148-54.