Evaluation of quantitative real-time PCR workflow modifications on 16S rRNA and tetA gene quantification in environmental samples

Science of the Total Environment

Volumn 426, Issue , 2012, Pages 351-358

  Nõlvak, Hiie ^a,b   Truu, Marika ^c   Truu, Jaak ^b,c  

^a UNIVERSITY OF TARTU   (Estonia)

^b Competence Centre on Reproductive Medicine and Biology ^*  (Estonia)

^c UNIVERSITY OF TARTU   (Estonia)

Author keywords

Amplification efficiency; Inhibition; LUX ; Real time quantitative PCR; SYBR green

Indexed keywords

16S RIBOSOMAL RNA; 16S RRNA; 16S RRNA GENE; AMPLICONS; DATA EVALUATION; DATA QUALITY; DATA SETS; DNA EXTRACTION; ENVIRONMENTAL SAMPLE; GENE COPY NUMBER; LOW DETECTION LIMIT; MICROBIAL COMMUNITIES; PRECISE METHOD; PRIMARY FACTORS; QUANTITATIVE POLYMERASE CHAIN REACTION; QUANTITATIVE REAL TIME PCR; REAL-TIME QUANTITATIVE PCR; SAMPLE SETS; SOIL SAMPLE; SYBR GREEN; TARGET GENES;

EFFICIENCY; ENZYME INHIBITION; GENES; RNA; STATISTICS;

POLYMERASE CHAIN REACTION;

DNA; RNA 16S;

AMPLIFICATION; DNA; EFFICIENCY MEASUREMENT; INHIBITION; MICROBIAL COMMUNITY; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; RNA;

AMPLICON; ARTICLE; DNA EXTRACTION; ENVIRONMENTAL MONITORING; GENE; GENE DOSAGE; GENE TARGETING; GENETIC VARIABILITY; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MICROBIAL COMMUNITY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SOIL ANALYSIS; TETA GENE; WORKFLOW;

ANTIPORTERS; BACTERIAL PROTEINS; BACTERIAL TYPING TECHNIQUES; ENVIRONMENTAL MONITORING; GENES, BACTERIAL; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; WORKFLOW;

EID: 84860687300     PISSN: 00489697     EISSN: 18791026     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2012.03.054     Document Type: Article

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