Quantification of genes encoding resistance to aminoglycosides, -lactams and tetracyclines in wastewater environments by real-time PCR

International Journal of Environmental Health Research

Volumn 19, Issue 3, 2009, Pages 219-230

  Borjesson, Stefan ^a   Dienues, Olaf ^b   Jarnheimer, Per Åke ^c   Olsen, Bjorn ^d,e   Matussek, Andreas ^b,f   Lindgren, Per Eric ^a  

^a LINKÖPING UNIVERSITY   (Sweden)

^b RYHOV COUNTY HOSPITAL   (Sweden)

^c KALMAR COUNTY HOSPITAL   (Sweden)

^d LINNAEUS UNIVERSITY   (Sweden)

^e UPPSALA UNIVERSITY HOSPITAL   (Sweden)

^f ST GÖRAN S HOSPITAL   (Sweden)

Author keywords

Aac(6') Ie+aph(2''); LUX real time PCR; MecA; Sewage pollution; TetA; TetB; Water pollutants; Water quality

Indexed keywords

AMINOGLYCOSIDE; ANTIBIOTIC AGENT; BETA LACTAM; DNA; TETRACYCLINE DERIVATIVE; ANTIINFECTIVE AGENT; SEWAGE;

ANTIBIOTIC RESISTANCE; BIOFILM; DETECTION METHOD; GENE EXPRESSION; POLYMERASE CHAIN REACTION; SLUDGE; WASTE FACILITY; WASTEWATER; WATER QUALITY;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BIOFILM; DNA EXTRACTION; ENVIRONMENT; GENETIC RESISTANCE; HOSPITAL WASTE; LANDFILL LEACHATE; NONHUMAN; PIPELINE; REAL TIME POLYMERASE CHAIN REACTION; SLUDGE; SOIL; WASTE WATER MANAGEMENT; WATER POLLUTANT; WATER QUALITY; CITY; GENETICS; HOSPITAL; MICROBIOLOGY; POLYMERASE CHAIN REACTION; SEWAGE; SWEDEN;

AMINOGLYCOSIDES; ANTI-BACTERIAL AGENTS; BETA-LACTAM RESISTANCE; BIOFILMS; CITIES; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; HOSPITALS; POLYMERASE CHAIN REACTION; SEWAGE; SWEDEN; TETRACYCLINE RESISTANCE; WASTE DISPOSAL, FLUID; WATER MICROBIOLOGY;

EID: 70449646582     PISSN: 09603123     EISSN: 13691619     Source Type: Journal    
DOI: 10.1080/09603120802449593     Document Type: Article

References (32)

1. Amann RI, Ludwig W, Schleifer K. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev. 59(1):143-169.
2. Aoshima H, Kimura A, Shibutani A, Okada C, Matsumiya Y, Kubo M. 2006. Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method. Appl Microbiol Biotechnol. 71(6):875-880.
3. Auerbach EA, Seyfried EE, McMahon KD. 2007. Tetracycline resistance genes in activated sludge wastewater treatment plants. Water Res. 41(5):1143-1151.
4. Blattner FR, Plunkett G, Bloch CA, Perna NT, Burland V, Riley M, Collado-Vides J, Glasner JD, Rode CK, Mayhew GF, Gregor J, Davis NW, Kirkpatrick HA, Goeden MA, Rose DJ, Mau B, Shao Y. 1997. The complete genome sequence of Escherichia coli K-12. Science. 277(5331): 1453-1474.
5. Chitnis V, Chitnis D, Patil S, Kant R. 2000. Hospital effluent: A source of multiple drug-resistant bacteria. Curr Sci. 79(7):989-991.
6. Davies J. 1994. Inactivation of antibiotics and the dissemination of resistance genes. Science. 264(5157):375-382.
7. Fluit AC, Visser MR, Schmitz FJ. 2001. Molecular detection of antimicrobial resistance. Clin Microbiol Rev. 14(4):836-871.
8. Esiobu N, Armenta L, Ike J. 2002. Antibiotic resistance in soil and water environments. Int J Environ Health Res. 12:133-144.
9. Gill SR, Fouts DE, Archer GL, Mongodin EF, Deboy RT, Ravel J, Paulsen IT, Kolonay JF, Brinkac L, Beanan M, Dodson RJ, Daugherty SC, Madupu R, Angiuoli SV, Durkin AS, Haft DH, Vamathevan J, Khouri H, Utterback T, Lee C, Dimitrov G, Jiang L, Qin H,Weidman J, Tran K, Kang K, Hance IR, Nelson KE, Fraser CM. 2005. Insights on evolution of virulence and resistance from the complete genome analysis of an early methicillin-resistant Staphylococcus aureus strain and a biofilm-producing methicillin-resistant Staphylococcus epidermidis strain. J Bacteriol. 187(7):2426-2438.
10. Guillaume G, Verbrugge D, Chasseur-Libotte M, Moens W, Collard J. 2000. PCR typing of tetracycline resistance determinants (Tet A-E) in Salmonella enterica serotype Hadar and in the microbial community of activated sludges from hospital and urban wastewater treatment facilities in Belgium. FEMS Microbiol Ecol. 32(1):77-85.
11. Halling-Sorensen B, Nors Nielsen S, Lanzky PF, Ingerslev F, Holten Lutzhoft HC, Jorgensen SE. 1998. Occurrence, fate and effects of pharmaceutical substances in the environment - a review. Chemosphere. 36(2):357-393.
12. Heuer H, Krogerrecklenfort E, Wellington EMH, Egan S, van Elsas JD, van Overbeek L, Collard JM, Guillaume G, Karagouni AD, Nikolakopoulou TL, Smalla K. 2002. Gentamicin resistance genes in environmental bacteria: Prevalence and transfer. FEMS Microbiol Ecol. 42(2):289-302.
13. Jarnheimer PA, Ottoson J, Lindberg R, Stenström TA, Johansson M, Tysklind M, Winner MM, Olsen B. 2004. Fluoroquinolone antibiotics in a hospital sewage line; occurrence, distribution and impact on bacterial resistance. Scand J Infect Dis. 36(10):752-755.
14. Kummerer K. 2004. Resistance in the environment. J Antimicrob Chemother. 54(2):311-320.
15. Levy SB. 1998. The challenge of antibiotic resistance. Sci Am. 278(3):46-53.
16. Levy SB, Marshall B. 2004. Antibacterial resistance worldwide: Causes, challenges and responses. Nat Med. 10(12):122-129.
17. Lindberg R, Jarnheimer PA, Olsen B, Johansson M, Tysklind M. 2004. Determination of antibiotic substances in hospital sewage water using solid phase extraction and liquid chromatography/mass spectrometry and group analogue internal standards. Chemosphere. 57(10):1479-1488.
18. Lowe B, Avila HA, Bloom FR, Gleeson M, Kusser W. 2003. Quantisation of gene expression in neural precursors by reverse-transcription polymerase chain reaction using self-quenched, fluorogenic primers. Anal Biochem. 315(1):95-105.
19. Lydmark P, Brunström R, Samuelsson K, Mattson A, Sörensson F, Lindgren PE, Hermansson M. 2007. Effects of environmental conditions on the nitrifying population dynamics in a pilot wastewater treatment plant. Environ Microbiol. 9(9):2220-2233.
20. Paulsen IT, Banerjei L, Myers GS, Nelson KE, Seshadri R, Read TD, Fouts DE, Eisen JA, Gill SR, Heidelberg JF, Tettelin H, Dodson RJ, Umayam L, Brinkac L, Beanan M, Daugherty S, DeBoy RT, Durkin S, Kolonay J, Madupu R, Nelson W, Vamathevan J, Tran B, Upton J, Hansen T, Shetty J, Khouri H, Utterback T, Radune D, Ketchum KA, Dougherty BA, Fraser CM. 2003. Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis. Science. 299(5615):2071-2074.
21. Pauwels B, Verstraete W. 2006. The treatment of hospital wastewater: An appraisal. J Water Health. 4(4):405-416.
22. Patterson AJ, Colangeli R, Spigaglia P, Scott KP. 2007. Distribution of specific tetracycline and erythromycin resistance genes in environmental samples assessed by macroarry detection. Environ Microbiol. 9(3):703-715.
23. Peak N, Knapp CW, Yang RK, Hanfelt MM, Smith MS, Aga DS, Graham DW. 2007. Abundance of six tetracycline resistance genes in wastewater lagoons at cattle feedlots with different antibiotic use strategies. Environ Microbiol. 9(1):143-151.
24. Reinthaler FF, Posch J, Feierl G, Wust G, Haas D, Ruckenbauer G, Mascher F, Marth E. 2003. Antibiotic resistance of E. coli in sewage and sludge. Water Res. 37(8):1685-1690.
25. Rhodes G, Huys G, Swings J, McGann P, Hiney M, Smith P, Pickup RW. 2000. Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: Implication of Tn1721 in dissemination of the tetracycline resistance determinant tetA. Appl Environ Microbiol. 66(9):3883-3890.
26. Rice EW, Messer JW, Johnson CH, Reasoner DJ. 1995. Occurrence of high-level aminoglycoside resistance in environmental isolates of enterococci. Appl Environ Microbiol. 61(1):374-376.
27. Roberts MC. 1996. Tetracycline resistance determinants: Mechanisms of action, regulation of expression, genetic mobility, and distribution. FEMS Microbiol Rev. 19(1):1-24.
28. Schwartz T, Kohnen W, Jansen B, Obst U. 2003. Detection of antibiotic-resistant bacteria and their resistance genes in wastewater, surface water, and drinking water biofilms. FEMS Microbiol Ecol. 43(3):325-335.
29. Strindhall J, Lindgren PE, Lofgren S, Kihlstrom E. 2002. Variations among clinical isolates of Staphylococcus aureus to induce expression of E-selectin and ICAM-1 in human endothelial cells. FEMS Immunol. Med Microbiol. 32(3):227-235.
30. Sundberg C, Stendahl JSK, Tonderski K, Lindgren PE. 2007. Overland flow systems for treatment of landfill leachates - potential nitrification and structure of the ammonia-oxidising bacterial community during a growing season. Soil Biol Biochem. 39:127-138.
31. Sundsfjord A, Simonsen GS, Haldorsen BC, Haaheim H, Hjelmevoll SO, Littauer P, Dahl KH. 2004. Genetic methods for detection of antimicrobial resistance. APMIS 112(11-12):815-837.
32. Volkmann H, Schwartz T, Bischoff P, Kirchen S, Obst U. 2004. Detection of clinically relevant antibiotic-resistance genes in municipal wastewater using real-time PCR (TaqMan). J Microbiol Meth. 56(2):277-286.