Probing the 3-D structure, dynamics, and stability of bacterial collagenase collagen binding domain (apo- versus holo-) by limited proteolysis MALDI-TOF MS

Journal of the American Society for Mass Spectrometry

Volumn 23, Issue 3, 2012, Pages 505-519

  Sides, Cynthia R ^a   Liyanage, Rohana ^a   Lay, Jackson O ^a   Philominathan, Sagaya Theresa Leena ^a,c   Matsushita, Osamu ^b   Sakon, Joshua ^a  

^a UNIVERSITY OF ARKANSAS   (United States)

^b KITASATO UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^c ALEXION PHARMACEUTICALS   (United States)

Author keywords

3D structure; B factors; Collagen; Collagen binding domain; Limited proteolysis; MALDI TOF MS; Mapping protein surface; Secondary structure; Solution dynamics; Stability; X ray structure; Helix; sheets

Indexed keywords

CALCIUM; COBALT; CRYSTAL STRUCTURE; DESORPTION; DYNAMICS; INDUCTIVELY COUPLED PLASMA; MASS SPECTROMETRY; PROTEOLYSIS; STABILITY; X RAY SCATTERING;

3D STRUCTURE; B FACTORS; COLLAGEN BINDING DOMAIN; LIMITED PROTEOLYSIS; MAPPING PROTEIN SURFACES; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; SECONDARY STRUCTURES; SOLUTION DYNAMICS; X-RAY STRUCTURE; Α HELIX; Β SHEET;

COLLAGEN;

CALCIUM; COBALT; COLLAGENASE; APOENZYME; CLOSTRIDIOPEPTIDASE A; GLUTAMYL ENDOPEPTIDASE; PEPTIDE FRAGMENT; SERINE PROTEINASE; TRYPSIN;

ALPHA HELIX; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BETA SHEET; CALCIUM BINDING; CLOSTRIDIUM HISTOLYTICUM; COLLAGEN BINDING DOMAIN; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CRYSTAL STRUCTURE; DYNAMICS; ENZYME SPECIFICITY; LIMITED PROTEOLYSIS; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; METAL BINDING; PROTEIN CLEAVAGE; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; PROTEIN STRUCTURE; X RAY CRYSTALLOGRAPHY; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; PROTEIN CONFORMATION;

APOENZYMES; BINDING SITES; CALCIUM; CLOSTRIDIUM HISTOLYTICUM; COBALT; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; MICROBIAL COLLAGENASE; MODELS, MOLECULAR; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; SERINE ENDOPEPTIDASES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPSIN;

EID: 84860578699     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-011-0309-3     Document Type: Article

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