Two-photon imaging of microglia in the mouse cortex in Vivo

Cold Spring Harbor Protocols

Volumn 7, Issue 5, 2012, Pages 594-603

  Nimmerjahn, Axel ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; BRAIN CORTEX; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; IN VIVO STUDY; MICROGLIA; MOUSE; NONHUMAN; TIME LAPSE IMAGING; TRANSGENIC MOUSE; ANIMAL; CYTOLOGY; GENETICS; IMMUNOLOGY; PHYSIOLOGY; SKULL; STAINING;

MUS;

FLUORESCENT DYE; PHOTOPROTEIN;

ANIMALS; CEREBRAL CORTEX; FLUORESCENT DYES; LUMINESCENT PROTEINS; MICE; MICROGLIA; MICROSCOPY, FLUORESCENCE; SKULL; STAINING AND LABELING;

EID: 84860564770     PISSN: 19403402     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot069294     Document Type: Article

References (39)

1. Bacskai BJ, Kajdasz ST, Christie RH, Carter C, Games D, Seubert P, Schenk D, Hyman BT. 2001. Imaging of amyloid-b deposits in brains of living mice permits direct observation of clearance of plaques with immunotherapy. Nat Med 7: 369-372.
2. Bolmont T, Haiss F, Eicke D, Radde R, Mathis CA, Klunk WE, Kohsaka S, Jucker M, Calhoun ME. 2008. Dynamics of the microglial/amyloid interaction indicate a role in plaque maintenance. J Neurosci 28: 4283-4292.
3. Christie RH, Bacskai BJ, Zipfel WR, Williams RM, Kajdasz ST, Webb WW, Hyman BT. 2001. Growth arrest of individual senile plaques in a model of Alzheimer's disease observed by in vivo multiphoton microscopy. J Neurosci 21: 858-864.
4. Davalos D, Grutzendler J, Yang G, Kim JV, Zuo Y, Jung S, Littman DR, Dustin ML, Gan WB. 2005. ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci 8: 752-758.
5. Dombeck DA, Khabbaz AN, Collman F, Adelman TL, Tank DW. 2007. Imaging large-scale neural activity with cellular resolution in awake, mobile mice. Neuron 56: 43-57.
6. Donnert G, Eggeling C, Hell SW. 2009. Triplet-relaxation microscopy with bunched pulsed excitation. Photochem Photobiol Sci 8: 481-485.
7. Engelbrecht CJ, Göbel W, Helmchen F. 2009. Enhanced fluorescence signal in nonlinear microscopy through supplementary fiber-optic light collection. Opt Express 17: 6421-6435.
8. Garcia-Alloza M, Bacskai BJ. 2004. Techniques for brain imaging in vivo. Neuromolecular Med 6: 65-78.
9. Greenberg DS, Kerr JN. 2009. Automated correction of fast motion artifacts for two-photon imaging of awake animals. J Neurosci Methods 176: 1-15.
10. Grutzendler J, Kasthuri N, Gan WB. 2002. Long-term dendritic spine stability in the adult cortex. Nature 420: 812-816.
11. Haynes SE, Hollopeter G, Yang G, Kurpius D, Dailey ME, Gan WB, Julius D. 2006. The P2Y12 receptor regulates microglial activation by extracellular nucleotides. Nat Neurosci 9: 1512-1519.
12. Helm PJ, Ottersen OP, Nase G. 2009. Analysis of optical properties of the mouse cranium-Implications for in vivo multiphoton laser scanning microscopy. J Neurosci Methods 178: 316-322.
13. Helmchen F, Denk W. 2005. Deep tissue two-photon microscopy. Nat Methods 2: 932-940.
14. Holtmaat A, Bonhoeffer T, Chow DK, Chuckowree J, De Paola V, Hofer SB, Huebener M, Keck T, Knott G, Lee W-CA, et al. 2009. Long-term, highresolution imaging in the mouse neocortex through a chronic cranial window. Nat Protoc 4: 1128-1144.
15. Hopt A, Neher E. 2001. Highly nonlinear photodamage in two-photon fluorescence microscopy. Biophys J 80: 2029-2036.
16. Ji N, Magee JC, Betzig E. 2008. High-speed, low-photodamage nonlinear imaging using passive pulse splitters. Nat Methods 5: 197-202.
17. Kawamura S, Schuerer L, Goetz A, Kempski O, Schmucker B, Baethmann A. 1990. An improved closed cranial window technique for investigation of blood-brain barrier function and cerebral vasomotor control in the rat. Int J Microcirc Clin Exp 9: 369-383.
18. Kim JV, Dustin ML. 2006. Innate response to focal necrotic injury inside the blood-brain barrier. J Immunol 177: 5269-5277.
19. Koenigsknecht-Talboo J, Meyer-Luehmann M, Parsadanian M, Garcia-Alloza M, Finn MB, Hyman BT, Bacskai BJ, Holtzman DM. 2008. Rapid microglial response around amyloid pathology after systemic anti-Aβ antibody administration in PDAPP mice. J Neurosci 28: 14156-14164.
20. Koester HJ, Baur D, Uhl R, Hell SW. 1999. Ca2+ fluorescence imaging with picoand femtosecond two-photon excitation: Signal and photodamage. Biophys J 77: 2226-2236.
21. Lalancette-Hebert M, Phaneuf D, Soucy G, Weng YC, Kriz J. 2009. Live imaging of Toll-like receptor 2 response in cerebral ischaemia reveals a role of olfactory bulb microglia as modulators of inflammation. Brain 132: 940-954.
22. Mandrekar S, Jiang QG, Lee CYD, Koenigsknecht-Talboo J, Holtzman DM, Landreth GE. 2009. Microglia mediate the clearance of soluble Ab through fluid phase macropinocytosis. J Neurosci 29: 4252-4262.
23. Navari RM, Wei EP, Kontos HA, Patterson JL. 1978. Comparison of the open skull and cranial window preparations in the study of the cerebral microcirculation. Microvasc Res 16: 304-315.
24. Nguyen QT, Tsai PS, Kleinfeld D. 2006. MPScope: A versatile software suite for multiphoton microscopy. J Neurosci Methods 156: 351-359.
25. Nimmerjahn A. 2012a. Surgical implantation of a head plate in mice in preparation for in vivo two-photon imaging of microglia. Cold Spring Harb. doi: 10.1101/pdb.prot069278.
26. Nimmerjahn A. 2012b. Optical window preparation for two-photon imaging of microglia in mice. Cold Spring Harb Protoc doi: 10.1101/pdb.prot069286.
27. Nimmerjahn A, Kirchhoff F, Helmchen F. 2005. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science 308: 1314-1318.
28. Nimmerjahn A, Theer P, Helmchen F. 2008. Two-photon laser scanning microscopy. In Ultrashort laser pulses in biology and medicine (ed Braun M, Gilch P, Zinth W), pp. 29-51.
29. Springer-Verlag, Heidelberg. Nimmerjahn A, Mukamel EA, Schnitzer MJ. 2009. Motor behavior activates Bergmann glial networks. Neuron 62: 400-412.
30. Oheim M, Beaurepaire E, Chaigneau E, Mertz J, Charpak S. 2001. Twophoton microscopy in brain tissue: Parameters influencing the imaging depth. J Neurosci Methods 111: 29-37.
31. Raivich G, Bohatschek M, Kloss CUA, Werner A, Jones LL, Kreutzberg GW. 1999. Neuroglial activation repertoire in the injured brain: Graded response, molecular mechanisms, and cues to physiological function. Brain Res Rev 30: 77-105.
32. Ransohoff RM, Perry VH. 2009. Microglial physiology: Unique stimuli, specialized responses. Annu Rev Immunol 27: 119-145.
33. Rueckel M, Mack-Bucher JA, Denk W. 2006. Adaptive wavefront correction in two-photon microscopy using coherence-gated wavefront sensing. Proc Natl Acad Sci 103: 17137-17142.
34. Spiess E, Bestvater F, Heckel-Pompey A, Toth K, Hacker M, Stobrawa G, Feurer T, Wotzlaw C, Berchner-Pfannschmidt U, Porwol T, et al. 2005. Two-photon excitation and emission spectra of the green fluorescent protein variants ECFP, EGFP, and EYFP. J Microsc 217: 200-204.
35. Tang S, Krasieva TB, Chen Z, Tempea G, Tromberg BJ. 2006. Effect of pulse duration on two-photon excited fluorescence and second harmonic generation in nonlinear optical microscopy. J Biomed Opt 11: 020501.
36. Wake H, Moorhouse AJ, Jinno S, Kohsaka S, Nabekura J. 2009. Resting microglia directly monitor the functional state of synapses in vivo and determine the fate of ischemic terminals. J Neurosci 29: 3974-3980.
37. Walter ML, Domes ME, Diller RA, Sproedt J, Joosten UH. 1999. Photoablation of bone by excimer laser radiation. Lasers Surg Med 25: 153-158.
38. Wiart M, Davoust N, Pialat J-B, Desestret V, Moucharrafie S, Cho T-H, Mutin M, Langlois J-B, Beuf O, Honnorat J, et al. 2006. MRI monitoring of neuroinflammation in mouse focal ischemia. Stroke 38: 131-137.
39. Xu HT, Pan F, Yang G, Gan WB. 2007. Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex. Nat Neurosci 10: 549-551.