Highly efficient large-scale lentiviral vector concentration by tandem tangential flow filtration

Journal of Virological Methods

Volumn 177, Issue 1, 2011, Pages 1-9

  Cooper, Aaron R ^a   Patel, Sanjeet ^a,b   Senadheera, Shantha ^a   Plath, Kathrin ^a,b   Kohn, Donald B ^a   Hollis, Roger P ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Genomic DNA normalization; IPS cells; Large scale; Lentiviral vector concentration; Tangential flow filtration

Indexed keywords

BUTYRIC ACID; CD34 ANTIGEN; LENTIVIRUS VECTOR;

ANIMAL CELL; CONCENTRATION PROCESS; CONTROLLED STUDY; CROSSFLOW FILTRATION; EMBRYO; FEMALE; FIBROBLAST; GENE DOSAGE; GENETIC TRANSDUCTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; LARGE SCALE PRODUCTION; MALE; MOUSE; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PRODUCT RECOVERY; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; SUPERNATANT; TANDEM TANGENTIAL FLOW FILTRATION; TIME; TOXICITY; VIRAL GENE DELIVERY SYSTEM; VIRUS TITRATION;

ANIMALS; ANTIGENS, CD34; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; CENTRIFUGATION; FIBROBLASTS; FILTRATION; GENETIC VECTORS; HEMATOPOIETIC STEM CELLS; HT29 CELLS; HUMANS; INDUCED PLURIPOTENT STEM CELLS; LENTIVIRUS; MICE; TRANSDUCTION, GENETIC;

EID: 84860417691     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.06.019     Document Type: Review

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