HuR post-transcriptionally regulates TNF-α-induced IL-6 expression in human pulmonary microvascular endothelial cells mainly via tristetraprolin

Respiratory Physiology and Neurobiology

Volumn 181, Issue 2, 2012, Pages 154-161

  Shi, Jia Xin ^a   Su, Xin ^a   Xu, Jin ^a   Zhang, Wei Yun ^b   Shi, Yi ^a  

^a MEDICAL SCHOOL OF NANJING UNIVERSITY   (China)

^b NANJING UNIVERSITY   (China)

Author keywords

Human pulmonary microvascular endothelial cells; HuR; IL 6; Tristetraprolin

Indexed keywords

HUR PROTEIN; INTERLEUKIN 6; MESSENGER RNA; TRISTETRAPROLIN; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; CONTROLLED STUDY; CYTOKINE RELEASE; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LUNG BLOOD VESSEL; MICROVASCULAR ENDOTHELIAL CELL; POSTTRANSCRIPTIONAL GENE SILENCING; PRIORITY JOURNAL; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RNA STABILITY;

3' UNTRANSLATED REGIONS; CELL LINE; ENDOTHELIAL CELLS; GENE SILENCING; HU PARANEOPLASTIC ENCEPHALOMYELITIS ANTIGENS; HUMANS; INTERLEUKIN-6; LUNG; MICROVESSELS; RNA, SMALL INTERFERING; TRISTETRAPROLIN; TUMOR NECROSIS FACTOR-ALPHA;

EID: 84860352364     PISSN: 15699048     EISSN: 18781519     Source Type: Journal    
DOI: 10.1016/j.resp.2012.02.011     Document Type: Article

References (25)

1. Dean J.L., Wait R., Mahtani K.R., Sully G., Clark A.R., Saklatvala J. The 3' untranslated region of tumor necrosis factor alpha mRNA is a target of the mRNA-stabilizing factor HuR. Mol. Cell. Biol. 2001, 21:721-730.
2. Donahue J.M., Chang E.T., Xiao L., Wang P.Y., Rao J.N., Turner D.J., Wang J.Y., Battafarano R.J. The RNA-binding protein HuR stabilizes survivin mRNA in human oesophageal epithelial cells. Biochem. J. 2011, 437:89-96.
3. Fan X.C., Steitz J.A. Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs. EMBO J. 1998, 17:3448-3460.
4. Gray L.C., Hughes T.R., van den Berg C.W. Binding of human antigen R (HuR) to an AU-rich element (ARE) in the 3' untranslated region (3' UTR) reduces the expression of decay accelerating factor (DAF). Mol. Immunol. 2010, 47:2545-2551.
5. Hasegawa H., Kakuguchi W., Kuroshima T., Kitamura T., Tanaka S., Kitagawa Y., Totsuka Y., Shindoh M., Higashino F. HuR is exported to the cytoplasm in oral cancer cells in a different manner from that of normal cells. Br. J. Cancer 2009, 100:1943-1948.
6. Hierholzer C., Kalff J.C., Omert L., Tsukada K., Loeffert J.E., Watkins S.C., Billiar T.R., Tweardy D.J. Interleukin-6 production in hemorrhagic shock is accompanied by neutrophil recruitment and lung injury. Am. J. Physiol. 1998, 275:L611-L621.
7. Hinman M.N., Lou H. Diverse molecular functions of Hu proteins. Cell. Mol. Life Sci. 2008, 65:3168-3181.
8. Kawai T., Lal A., Yang X., Galban S., Mazan-Mamczarz K., Gorospe M. Translational control of cytochrome c by RNA-binding proteins TIA-1 and HuR. Mol. Cell. Biol. 2006, 26:3295-3307.
9. Keene J.D. Why is Hu where? Shuttling of early-response-gene messenger RNA subsets. Proc. Natl. Acad. Sci. U.S.A. 1999, 96:5-7.
10. Lai W.S., Carballo E., Thorn J.M., Kennington E.A., Blackshear P.J. Interactions of CCCH zinc finger proteins with mRNA. Binding of tristetraprolin-related zinc finger proteins to Au-rich elements and destabilization of mRNA. J. Biol. Chem. 2000, 275:17827-17837.
11. Leandersson K., Riesbeck K., Andersson T. Wnt-5a mRNA translation is suppressed by the Elav-like protein HuR in human breast epithelial cells. Nucl. Acids. Res. 2006, 34:3988-3999.
12. Lee H.H., Son Y.J., Lee W.H., Park Y.W., Chae S.W., Cho W.J., Kim Y.M., Choi H.J., Choi D.H., Jung S.W., Min Y.J., Park S.E., Lee B.J., Cha H.J., Park J.W. Tristetraprolin regulates expression of VEGF and tumorigenesis in human colon cancer. Int. J. Cancer 2010, 126:1817-1827.
13. Lejbkowicz F., Goldberg-Cohen I., Levy A.P. New horizons for VEGF. Is there a role for nuclear localization?. Acta Histochem. 2005, 106:405-411.
14. Lin F.Y., Chen Y.H., Lin Y.W., Tsai J.S., Chen J.W., Wang H.J., Chen Y.L., Li C.Y., Lin S.J. The role of human antigen R, an RNA-binding protein, in mediating the stabilization of toll-like receptor 4 mRNA induced by endotoxin: a novel mechanism involved in vascular inflammation. Arterioscler. Thromb. Vasc. Biol. 2006, 26:2622-2629.
15. Ma W.J., Cheng S., Campbell C., Wright A., Furneaux H. Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein. J. Biol. Chem. 1996, 271:8144-8151.
16. Park W.Y., Goodman R.B., Steinberg K.P., Ruzinski J.T., Radella F., Park D.R., Pugin J., Skerrett S.J., Hudson L.D., Martin T.R. Cytokine balance in the lungs of patients with acute respiratory distress syndrome. Am. J. Respir. Crit. Care Med. 2001, 164:1896-1903.
17. Sandler H., Stoecklin G. Control of mRNA decay by phosphorylation of tristetraprolin. Biochem. Soc. Trans. 2008, 36:491-496.
18. Su X., Ao L., Zou N., Song Y., Yang X., Cai G.Y., Fullerton D.A., Meng X. Post-transcriptional regulation of TNF-induced expression of ICAM-1 and IL-8 in human lung microvascular endothelial cells: an obligatory role for the p38 MAPK-MK2 pathway dissociated with HSP27. J. Biochim. Biophys. Acta 2008, 1783:1623-1631.
19. Tenenbaum S.A., Lager P.J., Carson C.C., Keene J.D. Ribonomics: identifying mRNA subsets in mRNP complexes using antibodies to RNA-binding proteins and genomic arrays. Methods 2002, 26:191-198.
20. Tran H., Maurer F., Nagamine Y. Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2. Mol. Cell. Biol. 2003, 23:7177-7188.
21. Van Tubergen E., Vander Broek R., Lee J., Wolf G., Carey T., Bradford C., Prince M., Kirkwood K.L., D'Silva N.J. Tristetraprolin regulates interleukin-6, which is correlated with tumor progression in patients with head and neck squamous cell carcinoma. Cancer 2011, 117:2677-2689. doi: 10.1002/cncr.25859.
22. Xu, J., Su, X., Shi, J.X., Zhang, Y., Sun, H., Sun, W.K., Shi, Y., Unpublished results. MK2 regulates TNF-α-induced IL-6 expression by HuR.
23. Young L.E., Sanduja S., Bemis-Standoli K., Pena E.A., Price R.L., Dixon D.A. The mRNA binding proteins HuR and tristetraprolin regulate cyclooxygenase 2 expression during colon carcinogenesis. Gastroenterology 2009, 136:1669-1679.
24. Zhao W., Liu M., D'Silva N.J., Kirkwood K.L. Tristetraprolin regulates interleukin-6 expression through p38 MAPK-dependent affinity changes with mRNA 3' untranslated region. J. Interferon Cytokine Res. 2011, 31:629-637. doi: 10.1089/jir.2010.0154.
25. Zhou H., Jarujaron S., Gurley E.C., Chen L., Ding H., Studer E., Pandak W.M., Hu W., Zou T., Wang J.Y., Hylemon P.B. HIV protease inhibitors increase TNF-alpha and IL-6 expression in macrophages: involvement of the RNA-binding protein HuR. Atherosclerosis 2007, 195:e134-e143.