A new mitochondrial pH biosensor for quantitative assessment of pancreatic β-cell function

Biochemical and Biophysical Research Communications

Volumn 421, Issue 1, 2012, Pages 20-26

  Ogata, Makiko ^a   Awaji, Takeo ^b   Iwasaki, Naoko ^a   Fujimaki, Risa ^a   Takizawa, Miho ^a   Maruyama, Kei ^b   Iwamoto, Yasuhiko ^a   Uchigata, Yasuko ^a  

^a TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

^b SAITAMA MEDICAL UNIVERSITY   (Japan)

Author keywords

Electron transport system; Insulinoma cell line; Mitochondrial membrane; PH

Indexed keywords

ADENOSINE TRIPHOSPHATE SENSITIVE POTASSIUM CHANNEL; CARBONYL CYANIDE CHLOROPHENYLHYDRAZONE; DIAZOXIDE; GLUCOSE; INSULIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ACIDIFICATION; ANIMAL CELL; ARTICLE; BIOSENSOR; ENERGY METABOLISM; FLUORESCENCE; GENETIC TRANSFECTION; INSULIN RELEASE; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL TARGETING SIGNAL; MOUSE; NONHUMAN; PANCREAS ISLET BETA CELL; PANCREAS ISLET CELL FUNCTION; PH MEASUREMENT; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT;

AMINOOXYACETIC ACID; ANIMALS; BIOSENSING TECHNIQUES; CALCIUM; CARBONYL CYANIDE M-CHLOROPHENYL HYDRAZONE; CELL LINE; DIAZOXIDE; GLUCOSE; HYDROGEN-ION CONCENTRATION; INSULIN; INSULIN-SECRETING CELLS; MICE; MITOCHONDRIA; PROTON IONOPHORES; RATS;

RATTUS;

EID: 84860351515     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2012.03.093     Document Type: Article

References (20)

1. Nicholls D., Budd S. Mitochondria and neuronal survival. Physiol. Rev. 2000, 80:315-360.
2. Akhmedov D., Braun M., Mataki C., Park K., Pozzan T., Schoonjans K., Rorsman P., Wollheim C., Wiederkehr A. Mitochondrial matrix pH controls oxidative phosphorylation and metabolism-secretion coupling in INS-1E clonal beta cells. fj.10-162222 [pii]. FASEB J. 2010, 11:4613-4626.
3. Llopis J., McCaffery J., Miyawaki A., Farquhar M., Tsien R. Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 1998, 95:6803-6808.
4. Awaji T., Hirasawa A., Shirakawa H., Tsujimoto G., Miyazaki S. Novel green fluorescent protein-based ratiometric indicators for monitoring pH in defined intracellular microdomains. S0006-291X(01), 96004-8 [pii]. Biochem. Biophys. Res. Commun. 2001, 289:457-462.
5. Nagai T., Ibata K., Park E.S., Kubota M., Mikoshiba K., Miyawaki A. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biologic applications. nbt0102-87 [pii]. Nat. Biotechnol. 2002, 20:87-90.
6. Asfari M., Janjic D., Meda P., Li G., Halban P.A., Wollheim C.B. Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines. Endocrinology 1992, 130:167-178.
7. Miyazaki J., Araki K., Yamato E., Ikegami H., Asano T., Shibasaki Y., Oka Y., Yamamura K. Establishment of a pancreatic beta cell line that retains glucose-inducible insulin secretion: special reference to expression of glucose transporter isoforms. Endocrinology 1990, 127:126-132.
8. Ozaki H., Sato K., Satoh T., Karaki H. Simultaneous recordings of calcium signals and mechanical activity using fluorescent dye fura 2 in isolated strips of vascular smooth muscle. Jpn. J. Pharmacol. 1987, 45:429-433.
9. Abad M., Di Benedetto G., Magalhes P., Filippin L., Pozzan T. Mitochondrial pH monitored by a new engineered green fluorescent protein mutant. M306766200 [pii]. J. Biol. Chem. 2004, 279:11521-11529.
10. 2+ influx. J. Biol. Chem. 1996, 271:8738-8746.
11. Wiederkehr A., Park K., Dupont O., Demaurex N., Pozzan T., Cline G., Wollheim C. Matrix alkalinization: a novel mitochondrial signal for sustained pancreatic beta-cell activation. emboj2008302 [pii]. EMBO J. 2009, 28:417-428.
12. Eto K., Tsubamoto Y., Terauchi Y., Sugiyama T., Kishimoto T., Takahashi N., Yamauchi N., Kubota N., Murayama S., Aizawa T., Akanuma Y., Aizawa S., Kasai H., Yazaki Y., Kadowaki T. Role of NADH shuttle system in glucose-induced activation of mitochondrial metabolism and insulin secretion. Science 1999, 283:981-985.
13. Arosio D., Ricci F., Marchetti L., Gualdani R., Albertazzi L., Beltram F. Simultaneous intracellular chloride and pH measurements using a GFP-based sensor. nmeth.1471 [pii]. Nat. Methods 2010, 7:516-518.
14. Deriy L.V., Gomez E.A., Jacobson D.A., Wang X., Hopson J.A., Liu X.Y., Zhang G., Bindokas V.P., Philipson L.H., Nelson D.J. The granular chloride channel ClC-3 is permissive for insulin secretion. Cell Metab. 2009, 10:316-323.
15. Li D.Q., Jing X., Salehi A., Collins S.C., Hoppa M.B., Rosengren A.H., Zhang E., Lundquist I., Olofsson C.S., Mörgelin M., Eliasson L., Rorsman P., Renström E. Suppression of sulfonylurea- and glucose-induced insulin secretion in vitro and in vivo in mice lacking the chloride transport protein ClC-3. Cell Metab. 2009, 10:309-315.
16. Heart E., Corkey R.F., Wikstrom J.D., Shirihai O.S., Corkey B.E. Glucose-dependent increase in mitochondrial membrane potential, but not cytoplasmic calcium, correlates with insulin secretion in single islet cells. Am. J. Physiol. Endocrinol. Metab. 2006, 290:E143-E148.
17. Gustavsson N., Abedi G., Larsson-Nyrén G., Lindström P. Timing of Ca2+ response in pancreatic beta-cells is related to mitochondrial mass. S0006-291X(05), 02884-6 [pii]. Biochem. Biophys. Res. Commun. 2006, 340:1119-1124.
18. Fex M., Nitert M., Wierup N., Sundler F., Ling C., Mulder H. Enhanced mitochondrial metabolism may account for the adaptation to insulin resistance in islets from C57BL/6J mice fed a high-fat diet. Diabetologia 2007, 50:74-83.
19. Lu H., Koshkin V., Allister E., Gyulkhandanyan A., Wheeler M. Molecular and metabolic evidence for mitochondrial defects associated with beta-cell dysfunction in a mouse model of type 2 diabetes. Diabetes 2010, 59:448-459.
20. Anello M., Lupi R., Spampinato D., Piro S., Masini M., Boggi U., Del Prato S., Rabuazzo A., Purrello F., Marchetti P. Functional and morphological alterations of mitochondria in pancreatic beta cells from type 2 diabetic patients. Diabetologia 2005, 48:282-289.