Efficient reconstitution of transcription elongation complexes for single-molecule studies of eukaryotic RNA polymerase II

Transcription

Volumn 3, Issue 3, 2012, Pages 146-153

  Palangat, Murali ^a,b   Larson, Matthew H ^c   Hu, Xiaopeng ^d   Gnatt, Averell ^d   Block, Steven M ^c   Landick, Robert ^b  

^a NCI   (United States)

^b UNIVERSITY OF WISCONSIN MADISON   (United States)

^c STANFORD UNIVERSITY   (United States)

^d UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

Author keywords

Optical trapping; Reconstitution; RNA polymerase II; Single molecule transcription; Transcription elongation complexes

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYTIDINE TRIPHOSPHATE; DNA FRAGMENT; GUANOSINE TRIPHOSPHATE; LIGASE; RNA POLYMERASE II;

3' UNTRANSLATED REGION; 5' UNTRANSLATED REGION; ARTICLE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DNA TEMPLATE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME RECONSTITUTION; ENZYME SYNTHESIS; EUKARYOTE; GEL MOBILITY SHIFT ASSAY; GENE; HUMAN; NONHUMAN; OPTICAL TWEEZERS; PROTEIN DNA BINDING; RNA TRANSCRIPTION; RNA TRANSLATION; RPB1 GENE; TRANSCRIPTION REGULATION; YEAST;

EID: 84860131275     PISSN: 21541264     EISSN: 21541272     Source Type: Journal    
DOI: 10.4161/trns.20269     Document Type: Article

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