Leukocytic responses and intestinal mucin dynamics of broilers protected with Enterococcus faecium EF55 and challenged with Salmonella Enteritidis

Research in Veterinary Science

Volumn 93, Issue 1, 2012, Pages 195-201

  Levkut, Martin ^a   Revajová, Viera ^a   Lauková, Andrea ^b   Ševčíková, Zuzana ^a   Spišáková, Viera ^a   Faixová, Zita ^a   Levkutová, Mária ^a   Strompfová, Viola ^b   Pistl, Juraj ^a   Levkut, Mikuláš ^a  

^a UNIVERSITY OF VETERINARY MEDICINE   (Slovakia)

^b INSTITUTE OF ANIMAL PHYSIOLOGY   (Slovakia)

Author keywords

Chickens; Intestine; Lymphocytes; Mucin; Probiotic microorganism; S. Enteritidis

Indexed keywords

CD3 ANTIGEN; CD4 ANTIGEN; CD8 ANTIGEN; IMMUNOGLOBULIN M; MUCIN; PROBIOTIC AGENT;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL SALMONELLOSIS; ANIMAL TISSUE; ANTIBACTERIAL ACTIVITY; ARTICLE; BACTERIAL COUNT; BROILER; CECUM; COAGULASE POSITIVE STAPHYLOCOCCUS; CONTROLLED STUDY; DIET SUPPLEMENTATION; DUODENUM; ENTEROBACTERIACEAE; ENTEROCOCCUS; ENTEROCOCCUS FAECIUM; ENTEROCOCCUS FAECIUM EF55; EOSINOPHIL COUNT; FECES ANALYSIS; HUMORAL IMMUNITY; ILEUM; INTESTINE FLORA; JEJUNUM; LEUKOCYTE COUNT; LYMPHOCYTE COUNT; LYMPHOCYTE FUNCTION; LYMPHOCYTE SUBPOPULATION; MACROPHAGE; MONOCYTE; NONHUMAN; PERIPHERAL LYMPHOCYTE; PHAGOCYTE; PHENOTYPE; QUANTITATIVE ANALYSIS; RANDOMIZATION; SALMONELLA ENTERITIDIS; SMALL INTESTINE;

ANIMALS; BACTERIAL LOAD; CHICKENS; DIETARY SUPPLEMENTS; ENTEROCOCCUS FAECIUM; FEMALE; FLOW CYTOMETRY; IMMUNITY, CELLULAR; INTESTINAL MUCOSA; LEUKOCYTE COUNT; LYMPHOCYTE SUBSETS; MUCINS; POULTRY DISEASES; PROBIOTICS; SALMONELLA ENTERITIDIS; SALMONELLA INFECTIONS, ANIMAL;

BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECIUM; GALLUS GALLUS; SALMONELLA; SALMONELLA ENTERITIDIS;

EID: 84859961056     PISSN: 00345288     EISSN: 15322661     Source Type: Journal    
DOI: 10.1016/j.rvsc.2011.06.021     Document Type: Article

References (30)

1. Asheg A.A., Levkut M., Revajová V., Ševčíková Z., Kolodzieyski L., Pistl J., Pilipčinec E. Spreading of Salmonella enteritidis in the cecum of chickens. Folia Microbiologica 2003, 48:277-279.
2. Barrow P.A., Simpson J.M., Lovell M.A. Intestinal colonization in the chicken by food-poisoning Salmonella serotypes: microbial characteristics associated with faecal excretion. Avian Pathology 1988, 17:571-588.
3. Boyum M.A. Separation of blood leukocytes, granulocytes and lymphocytes. Tissue Antigens 1974, 4:269-274.
4. Brownell J.R., Sadler W.W., Fanelli M.J. Role of ceca in intestinal infection of chickens with Salmonella thyphimurium. Avian Diseases 1970, 14:106-116.
5. Chambers J.R., Lu X. Probiotics and maternal vaccination for Salmonella control in broiler chickens. The Journal of Applied Poultry Research 2002, 11:320-327.
6. Chiu C.H., Ou J.T. Rapid identification of Salmonella serovars in faeces by specific detection of virulence genes, invA and spvC, by an enrichment broth culture - multiplex PCR combination assay. Journal of Clinical Microbiology 1996, 34:2619-2622.
7. Duchet-Suchaux M., Lechopier P., Marly J., Bernardet P., Delaunay R., Pardon P. Quantification of experimental S. enterica serovar Enteritidis carrier-state in B13 leghorn chicks. Avian Diseases 1995, 39:796-803.
8. Durant J.A., Nisbet D.J., Ricke S.C. Response of selected poultry cecal probiotic bacteria and a primary poultry Salmonella typhimurium isolate grown with or without glucose in liquid batch culture. Journal of Environmental Science and Health, Part B 2000, 35:503-516.
9. Erf G.F. Cell-mediated immunity in poultry. Poultry Science 2004, 83:580-590.
10. Fried K., JantoŠovič J. Blood sampling by cardiac punction. Veterinársky časopis 1961, 10:383-391. (In Slovak).
11. Herich R., Levkut M. Lactic acid bacteria, probiotics and immune system. Veterinary Medicine - Czech 2002, 47:169-180.
12. Karpus W.J. T cell receptor and CD3 antibody cross reactivity opens new research and veterinary medicine opportunities. Cytometry 2007, 71A:976-977.
13. Koutsos E.A., Arias V.J. Intestinal ecology: interactions among the gastrointestinal tract, nutrition, and the microflora. The Journal of Applied Poultry Research 2006, 15:161-173.
14. Lauková A., Guba P., Nemcová R., Mareková M. Inhibition of Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails. Veterinární Medicína 2004, 49:47-51.
15. Levkut M., Pistl J., Lauková A., Revajová V., Herich R., Ševčíková Z., Strompfová V., Szaboóvá R., Kokinčáková T. Antimicrobial activity of Enterococcus faecium EF55 against Salmonella Enteritidis in chickens. Acta Veterinaria Hungarica 2009, 57:13-24.
16. Macfarlane S., Woodmansey E.J., Macfarlane G.T. Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Applied and Environmental Microbiology 2005, 71:7483-7492.
17. Morency H., Mota-Meira M., Lapointe G., Lacroix C.H., Lavoie M.C. Comparison of the activity spectra against pathogens of bacterial strains producing a mutacin or a lantibiotic. Canadian Journal of Microbiology 2001, 47:322-331.
18. Popiel I.P., Turnbull C.B. Passage of Salmonella enteritidis and Salmonella thompson through the chick ileocaecal mucosa. Infection and Immunity 1995, 74:786-792.
19. Scott T.R. Our current understanding of humoral immunity of poultry. Poultry Science 2004, 83:574-579.
20. Sherman P.M., Ossa J., Johnson-Henry K. Unraveling mechanisms of action of probiotics. Nutrition in Clinical Practice 2009, 24:10-14.
21. Smirnov A., Sklan D., Uni Z. Mucin dynamics in the chick small intestine are altered by starvation. Journal of Nutrition 2004, 134:736-742.
22. Smirnov A., Perez R., Amit-Romach E., Sklan D., Uni Z. Mucin dynamics and microbial populations in chicken small intestine are changed by dietary probiotic and antibiotic growth promoter supplementation. Journal of Nutrition 2005, 135:187-192.
23. Snoeyenbos G.H., Soerjadi A.S., Weinack O.M. Gastrointestinal colonization by salmonellae and pathogenic Escherichia coli in monoxemic and holoxenic chicks. Avian Diseases 1982, 26:566-575.
24. Strompfová V., Lauková A. In vitro study on bacteriocin of enterococci associated with chickens. Anaerobe 2007, 13:228-237.
25. Strompfová V., Mudroňová D., Lauková A. Effect of bacteriocin-like substance produced by Enterococcus faecium EF55 on the composition of avian gastrointestinal microflora. Acta Veterinaria Brno 2003, 72:559-564.
26. Szabó I., Wieler L.H., Tedin K., Scharek-Tedin L., Taras D., Hensel A., Appel B., Nökler K. Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar Thyphimurium DT104 infection in a porcine animal infection model. Applied and Environmental Microbiology 2009, 75:2621-2628.
27. Thompson K.L., Applegate T.J. Feed withdrawal alters small-intestinal morphology and mucus of broilers. Poultry Science 2006, 85:1535-1540.
28. Tizard, I.R., 2009. Lymphocytes. In: Veterinary Immunology. An Introduction, eighth ed. Saunders Elsevier, St. Louis, Missouri, pp. 128-139.
29. Wigley P., Berchieri A., Page K.L., Smith A.L., Barrow P.A. Salmonella enterica serovar Pullorum persists in splenic macrophages and in the reproductive tract during persistent, disease-free carriage in chickens. Infection and Immunity 2001, 69:7873-7879.
30. Woodford N., Egelton M.C., Morrison D. Comparisons of PCR with phenotypic methods for the speciation of enterococci. Streptococci and the Host 1997, vol. 47:405-409. Plenum Press, New York.