Reducing progoitrin and enriching glucoraphanin in Braasica napus seeds through silencing of the GSL-ALK gene family

Plant Molecular Biology

Volumn 79, Issue 1-2, 2012, Pages 179-189

  Liu, Zheng ^a   Hirani, Arvind H ^a   McVetty, Peter B E ^a   Daayf, Fouad ^a   Quiros, Carlos F ^b   Li, Genyi ^a  

^a UNIVERSITY OF MANITOBA   (Canada)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Brassica napus; Gene silencing; Glucosinolates; GSL ALK genes

Indexed keywords

GLUCORAPHANIN; GLUCOSINOLATE; IMIDOESTER; PROGOITRIN;

ARTICLE; BIOSYNTHESIS; BRASSICA; CHEMISTRY; CROSS BREEDING; GENE EXPRESSION REGULATION; GENE SILENCING; GENE VECTOR; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MULTIGENE FAMILY; PLANT GENE; PLANT SEED; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING; TRANSGENIC PLANT;

BIOSYNTHETIC PATHWAYS; BLOTTING, SOUTHERN; BRASSICA; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHROMATOGRAPHY, LIQUID; CROSSES, GENETIC; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GENES, PLANT; GENETIC VECTORS; GLUCOSINOLATES; IMIDOESTERS; MASS SPECTROMETRY; MULTIGENE FAMILY; PLANTS, GENETICALLY MODIFIED; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEEDS;

ANIMALIA; BRASSICA; BRASSICA JUNCEA; BRASSICA NAPUS; BRASSICA RAPA;

EID: 84859949615     PISSN: 01674412     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11103-012-9905-2     Document Type: Article

References (25)

1. Beekwilder J, van Leeuwen W, van Dam NM, Bertossi M, Grandi V, Mizzi L, Soloviev M, Szabados L, Molthoff JW, Schipper B, Verbocht H, de Vos RC, Morandini P, Aarts MG, Bovy A (2008) The impact of the absence of aliphatic glucosinolates on insect herbivory in Arabidopsis. PLoS ONE 3: e2068.
2. Fahey JW, Zhang Y, Talalay P (1997) Broccoli sprouts: an exceptionally rich source of inducers of enzymes that protect against chemical carcinogens. Proc Natl Acad Sci USA 94: 10367-10372.
3. Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J, Terce F (2000) Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in Ht29 human colon cancer cells. Cancer Res 60: 1426-1433.
4. Gao M, Li G, Yang B, McCombie RW, Quiros CF (2004) Comparative analysis of a Brassica BAC clone containing several major aliphatic glucosinolate genes with its corresponding Arabidopsis sequence. Genome 47: 666-679.
5. Gigolashvili T, Yatusevich R, Berger B, Muller C, Flugge UI (2007) The R2R3-MYB transcription factor HAG1/MYB28 is a regulator of methionine-derived glucosinolate biosynthesis in Arabidopsis thaliana. Plant J 51: 247-261.
6. Kliebenstein DJ, Kroymann J, Brown P, Figuth A, Pedersen D, Gershenzon J, Mitchell-Olds T (2001) Genetic control of natural variation in Arabidopsis thaliana glucosinolate accumulation. Plant Physiol 126: 811-825.
7. Kushad MM, Brown AF, Kurilich AC, Juvik JA, Klein BP, Wallig MA, Jeffery EH (1999) Variation of glucosinolates in vegetable crops of Brassica oleracea. J Agric Food Chem 47: 1541-1548.
8. Li G, Quiros CF (2003) The Brassica oleracea gene BoGSL- ALK regulates in planta alkenyl aliphatic glucosinolates in Arabidopsis thaliana. Theor Appl Genet 106: 1116-1121.
9. Li G, Riaz A, Goyal S, Abel S, Quiros CF (2001) Inheritance of three major genes involved in the synthesis of aliphatic glucosinolates in Brassica oleracea. J Am Soc Hortic Sci 126: 427-431.
10. Li G, Gao M, Yang B, Quiros CF (2003) Gene to gene alignment between the Arabidopsis and Brassica oleracea genomes. Theor Appl Genet 107: 168-180.
11. Liu Z, Hammerlindl J, Keller W, McVetty PBE, Daayf F, Quiros CF, Li G (2010) MAM gene silencing leads to the induction of C3 and reduction of C4 and C5 side chain aliphatic glucosinolates in Brassica napus. Mol Breed 27: 467-478.
12. Lou P, Zhao J, He H, Hanhart C, Del Carpio DP, Verkerk R, Custers J, Koornneef M, Bonnema G (2008) Quantitative trait loci for glucosinolate accumulation in Brassica rapa leaves. New Phytol 179: 1017-1032.
13. Magrath R, Herron C, Giamoustaris A, Mithen R (1993) The inheritance of aliphatic glucosinolates in Brassica napus. Plant Breed 111: 55-72.
14. Mithen R, Faulkner K, Magrath R, Rose P, Williamson G, Marquez J (2003) Development of isothiocyanate-enriched broccoli, and its enhanced ability to induce phase 2 detoxification enzymes in mammalian cells. Theor Appl Genet 106: 727-734.
15. Moloney MM, Walker JM, Sharma KK (1989) High efficiency transformation of Brassica napus using Agrobacterium vectors. Plant Cell Rep 8: 238-242.
16. Neal CS, Fredericks DP, Griffiths CA, Neale AD (2010) The characterization of AOP2: a gene associated with biosynthesis of aliphatic alkenyl glucosinolates in Arabidopsis thaliana. BMC Plant Biol 10: 170-186.
17. Nour-Eldin HH, Hansen BG, Norholm MHH, Jensen JK, Halkier BA (2006) Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments. Nucleic Acids Res 34: e122.
18. Reintanz B, Lehnen M, Reichelt M, Gershenzon J, Kowalczyk M, Sandberg G, Godde M, Uhl R, Palme K (2001) Bus, a bushy Arabidopsis CYP79F1 knockout mutant with abolished synthesis of short-chain aliphatic glucosinolates. Plant Cell 13: 351-367.
19. Rosa EAS, Heaney RK, Fenwick GR, Portas CAM (1997) Glucosinolates in crop plants. Hortic Rev 19: 99-215.
20. Shapiro TA, Fahey JW, Wade KL, Stephenson KK, Talalay P (2001) Chemoprotective glucosinolates and isothiocyanates of broccoli sprouts: metabolism and excretion in humans. Cancer Epidemiol Biomarkers Prev 10: 501-508.
21. Shapiro TA, Fahey JW, Dinkova-Kostova AT et al (2006) Safety, tolerance, and metabolism of broccoli sprout glucosinolates and isothiocyanates: a clinical phase I study. Nutr Cancer 55: 53-62.
22. Sønderby IE, Hansen BG, Bjarnholt N, Ticconi C, Halkier BA et al (2007) A systems biology approach Identifies a R2R3 MYB gene subfamily with distinct and overlapping functions in regulation of aliphatic glucosinolates. PLoS ONE 2: e1322.
23. Velasco L, Becker HC (2000) Variability for seed glucosinolates in a germplasm collection of the genus Brassica. Genet Resour Crop Evol 47: 231-238.
24. Vinjamoori V, Byrum JR, Hayes T, Das PK (2004) Challenges and opportunities in the analysis of raffinose oligosaccharides, pentosans. J Anim Sci 82: 319-328.
25. Zhang GY, Talalay P, Cho C, Posner GH (1992) A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci USA 89: 2399-2403.