Development of a tetrameric streptavidin mutein with reversible biotin binding capability: Engineering a mobile loop as an exit door for biotin

PLoS ONE

Volumn 7, Issue 4, 2012, Pages

  O'Sullivan, Valerie J ^a   Barrette Ng, Isabelle ^b   Hommema, Eric ^a   Hermanson, Greg T ^a   Schofield, Mark ^a,c   Wu, Sau Ching ^b   Honetschlaeger, Claudia ^b   Ng, Kenneth K S ^b   Wong, Sui Lam ^b  

^a Thermo Fisher Scientific   (United States)

^b UNIVERSITY OF CALGARY   (Canada)

^c Pall Life Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTIN; MUTEIN; STREPTAVIDIN; UNCLASSIFIED DRUG; BACTERIAL PROTEIN; CYSTINE; IGG FC BINDING PROTEIN, STREPTOCOCCUS; IGG FC-BINDING PROTEIN, STREPTOCOCCUS; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BINDING KINETICS; BIOTINYLATION; CONTROLLED STUDY; DISULFIDE BOND; NONHUMAN; PLASMID; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN FUNCTION; PROTEIN IMMOBILIZATION; PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN SECRETION; PROTEIN STRUCTURE; WILD TYPE; X RAY CRYSTALLOGRAPHY; AFFINITY CHROMATOGRAPHY; AMINO ACID SUBSTITUTION; BINDING SITE; BIOSYNTHESIS; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN QUATERNARY STRUCTURE;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BINDING SITES; BIOTIN; CHROMATOGRAPHY, AFFINITY; CRYSTALLOGRAPHY, X-RAY; CYSTINE; KINETICS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN INTERACTION DOMAINS AND MOTIFS; PROTEIN STRUCTURE, QUATERNARY; RECOMBINANT PROTEINS; STREPTAVIDIN;

EID: 84859834620     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0035203     Document Type: Article

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