An automated microscale platform for evaluation and optimization of oxidative bioconversion processes

Biotechnology Progress

Volumn 28, Issue 2, 2012, Pages 392-405

  Baboo, Jasmin Z ^a   Galman, James L ^a   Lye, Gary J ^a   Ward, John M ^a   Hailes, Helen C ^a   Micheletti, Martina ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Automated processing; Cyclohexanone monooxygenase; High throughput; Microscale bioprocessing; Oxidative bioconversion

Indexed keywords

ASYMMETRIC SYNTHESIS; AUTOMATED PROCESS; AUTOMATED PROCESSING; BIOCONVERSION PROCESS; BIOPROCESS DEVELOPMENT; BIOPROCESSING; CYCLOHEXANONE MONOOXYGENASE; CYCLOHEXANONES; E. COLI; HIGH THROUGHPUT; HIGH-THROUGHPUT APPROACHES; KINETIC DATA; MANUAL INTERVENTION; MEDIA FORMULATIONS; MICRO-SCALES; MICROBIAL CULTURE; MICROSCALE PROCESSING; MICROTITER PLATES; MODEL SUBSTRATES; NATURAL SUBSTRATES; NORCAMPHOR; OXYGEN LIMITATION; OXYGEN TRANSFER COEFFICIENT; PRODUCT SELECTIVITIES; QUANTITATIVE DATA; ROBOTIC PLATFORMS; SCALE-UP; SQUARE-WELL; SUBSTRATE SPECIFICITY;

AMINO ACIDS; AUTOMATION; BIOCONVERSION; ESCHERICHIA COLI; OPTIMIZATION; OXYGEN; ROBOTICS;

SUBSTRATES;

BACTERIAL PROTEIN; CYCLOHEXANONE OXYGENASE; OXYGEN; OXYGENASE;

ACINETOBACTER CALCOACETICUS; ARTICLE; BIOREACTOR; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; EVALUATION; FERMENTATION; GENE EXPRESSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; INSTRUMENTATION; KINETICS; METABOLISM; METHODOLOGY; MICROBIOLOGY; OXIDATION REDUCTION REACTION; ROBOTICS;

ACINETOBACTER CALCOACETICUS; BACTERIAL PROTEINS; BIOREACTORS; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; KINETICS; OXIDATION-REDUCTION; OXYGEN; OXYGENASES; ROBOTICS;

ESCHERICHIA COLI;

EID: 84859648591     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1500     Document Type: Article

References (53)

1. Micheletti M, Lye GJ. Microscale bioprocess optimisation. Curr Opin Biotechnol. 2006; 17: 611-618.
2. Major J. Challenges and opportunities in high throughput screening: implications for new technologies. J Biomol Screen. 1998; 3: 13-17.
3. Sundberg SA. High-throughput and ultra-high-throughput screening: solution- and cell-based approaches. Curr Opin Biotechnol. 2000; 11: 47-53.
4. Persidis A. High throughput screening. Nat Biotechnol. 1998; 16: 488-489.
5. Duetz WA, Ruedi L, Hermann R, O'Connor K, Buchs J, Witholt B. Methods for intense aeration, growth, storage, and replication of bacterial strains in microtiter plates. Appl Environ Microbiol. 2000; 66: 2641-2646.
6. Minas W, Bailey JE, Duetz W. Streptomycetes in micro-cultures: growth, production of secondary metabolites, and storage and retrieval in the 96-well format. Antonie Van Leeuwenhoek. 2000; 78: 297-305.
7. Elmahdi I, Baganz F, Dixon K, Harrop T, Sugden A, Lye GL. pH control in microwell fermentations of S. erythraea CA340: influence on biomass growth kinetics and erythromycin biosynthesis. Biochem Eng J. 2003; 16: 299-310.
8. Girard P, Jordan M, Tsao M, Wurm FM. Small-scale bioreactor system for process development and optimization. Biochem Eng J. 2001; 7: 117-119.
9. Stahl SR, Greasham O, Chartrain M. Implementation of a rapid microbial screening procedure for biotransformation activities. J Biosci Bioeng. 2000; 89: 367-371.
10. John GT, Heinzle E. Quantitative screening method for hydrolases in microplates using pH indicators: determination of kinetic parameters by dynamic pH monitoring. Biotechnol Bioeng. 2001; 72: 620-627.
11. Islam R, Tisi D, Levy M, Lye G. Scale-up of Escherichia coli growth and recombinant protein expression conditions from microwell to laboratory and pilot scale based on matched kLa. Biotechnol Bioeng. 2008; 99: 1128-1139.
12. Jackson NB, Liddell JM, Lye GJ. An automated microscale technique for the quantitative and parallel analysis of microfiltration operations. J Membr Sci. 2006; 276: 31-41.
13. Rayat ACME, Micheletti M, Lye GJ. Evaluation of cell disruption effects on primary recovery of antibody fragments using microscale bioprocessing techniques. Biotechnol Progr. 2010; 26: 1312-1321.
14. Welch CJ, Shaimi M, Biba M, Chilenski JR, Dolling U, Mathre DJ, Reider PJ. Microplate evaluation of process adsorbents. J Sep Sci. 2002; 25: 847-850.
15. Lye GJ, Ayazi-Shamlou P, Baganz F, Dalby PA, Woodley JM. Accelerated design of bioconversion processes using automated microscale processing techniques. Trends Biotechnol. 2003; 21: 29-37.
16. Ferreira-Torres C, Micheletti M, Lye G. Microscale process evaluation of recombinant biocatalyst libraries: application to Baeyer-Villiger monooxygenase catalysed lactone synthesis. Bioprocess Biosyst Eng. 2005; 28: 83-93.
17. Huber R, Ritter D, Hering T, Hillmer A, Kensy F, Muller C, Wang L, Buchs J. Robo-Lector-a novel platform for automated high-throughput cultivations in microtiter plates with high information content. Microb Cell Fact. 2009; 8: 42.
18. Strukul G. Transition metal catalysis in the Baeyer-Villiger oxidation of ketones. Angew Chem Int Ed. 1998; 37: 1198-1209.
19. Zambianchi F, Pasta P, Ottolina G, Carrea G, Colonna S, Gaggero N, Ward JM. Effect of substrate concentration on the enantioselectivity of cyclohexanone monooxygenase from Acinetobacter calcoaceticus and its rationalization. Tetrahedron Asymmetr. 2000; 11: 3653-3657.
20. Baldwin CV, Woodley JM. On oxygen limitation in a whole cell biocatalytic Baeyer-Villiger oxidation process. Biotechnol Bioeng. 2006; 95: 362-369.
21. Berezina N, Kozma E, Furstoss R, Alphand V. Asymmetric Baeyer-Villiger biooxidation of alpha-substituted cyanocyclohexanones: influence of the substituent length on regio- and enantioselectivity. Adv Synth Catal. 2007; 349: 2049-2053.
22. Doig SD, Pickering SCR, Lye GJ, Woodley JM. The use of microscale processing technologies for quantification of biocatalytic Baeyer-Villiger oxidation kinetics. Biotechnol Bioeng. 2002; 80: 42-49.
23. Ferreira-Torres C. Microscale process characterisation of oxidative conversions, PhD Thesis, University College London, 2008.
24. Roberts SM, Wan PWH. Enzyme-catalysed Baeyer-Villiger oxidations. J Mol Catal B-Enzym. 1998; 4: 111-136.
25. Mihovilovic MD, Rudroff F, Grotzl B, Stanetty P. Microbial Baeyer-Villiger oxidation of prochiral polysubstituted cyclohexanones by recombinant whole-cells expressing two bacterial monooxygenases. Eur J Org Chem. 2005; 2005: 809-816.
26. Cernuchova P, Mihovilovic MD. Microbial Baeyer-Villiger oxidation of terpenones by recombinant whole-cell biocatalysts formation of enantiocomplementary regioisomeric lactones. Org Biomol Chem. 2007; 5: 1715.
27. Geitner K, Kirschner A, Rehdorf J, Rehdorf J, Schmidt M, Mihovilovic MD, Bornscheuer UT. Enantioselective kinetic resolution of 3-phenyl-2-ketones using Baeyer-Villiger monooxygenases. Tetrahedron Asymmetr. 2007; 18: 892-895.
28. Doig SD, O'Sullivan LM, Patel S, Ward JM, Woodley JM. Large scale production of cyclohexanone monooxygenase from Escherichia coli TOP10 pQR239. Enzyme Microb Technol. 2001; 28: 265-274.
29. Doig SD, Simpson H, Alphand V, Furstoss R, Woodley JM. Characterization of a recombinant Escherichia coli TOP10 [pQR239] whole-cell biocatalyst for stereoselective Baeyer-Villiger oxidations. Enzyme Microb Technol. 2003; 32: 347-355.
30. Alphand V, Carrea G, Roland W, Roland F, Woodley JM. Towards large-scale synthetic applications of Baeyer-Villiger monooxygenases. Trends Biotechnol. 2003; 21: 318-323.
31. Ottolina G, de Gonzalo G, Carrea G, Danieli B. Enzymatic Baeyer-Villiger oxidation of bicyclic diketones. Adv Synth Catal. 2005; 347: 1035-1040.
32. Nealon AJ, O'Kennedy RD, Titchener-Hooker NJ, Lye GJ. Quantification and prediction of jet macro-mixing times in static microwell plates. Chem Eng Sci. 2005; 61: 4860-4870.
33. Olsen K. A quantitative study of factors governing automatic pipetting performance. Am Lab. 2000; 32: 49-51.
34. Hussain HA, Ward JM. Enhanced heterologous expression of two Streptomyces griseolus Cytochrome P450s and Streptomyces coelicolor ferredoxin reductase as potentially efficient hydroxylation catalysts. Appl Environ Microbiol. 2002; 69: 373-382.
35. Roberts GA, Grogan G, Greter A, Flitsch SL, Turner NJ. Identification of a new class of cytochrome P450 from a Rhodococcus sp. J Bacteriol. 2002; 184: 3898-3908.
36. Duetz WA, Witholt B. Effectiveness of orbital shaking for the aeration of suspended bacterial cultures in square-deepwell microtiter plates. Biochem Eng J. 2001; 7: 113-115.
37. Kamerbeek N, Janssen DB, Van Berkel W, Fraaije MW. Baeyer-Villiger monooxygenases, an emerging family of flavin-dependent biocatalysts. Adv Synth Catal. 2003; 345: 667-678.
38. Newton RF, Roberts SM. Steric control in prostaglandin synthesis involving bicyclic and tricyclic intermediates. Tetrahedron. 1980; 36: 2163-2196.
39. Levitt MS, Newton RF, Roberts SM, Willetts AJ. Preparation of optically active 6'-fluorocarbocyclic nucleosides utilising an enantiospecific enzyme-catalysed Baeyer-Villiger type oxidation. J Chem Soc Chem Commun. 1990; 8: 619-620.
40. Hasegawa Y, Hamano K, Obata H, Tokuyama T. Microbial degradation of cycloheptanone. Agr Biol Chem. 1982; 45: 1139-1143.
41. Abril O, Ryerson CC, Walsh C, Whitesides GM. Enzymatic Baeyer-Villiger type oxidations of ketones catalyzed by cyclohexanone oxygenase. Bioorg Chem. 1989; 17: 41-52.
42. Mihovilovic M, Kapitán P, Kapitánová P. Regiodivergent Baeyer-Villiger oxidation of fused ketones by recombinant whole-cell biocatalysts. ChemSusChem. 2008; 1: 143-148.
43. Kim Y, Jung S, Chung Y, Yu C, Rhee I. Cloning and characterization of a cyclohexanone monooxygenase gene from Arthrobacter sp. L661. Biotechnol Bioprocess Eng. 2008; 13: 40-47.
44. Trower M, Buckland M, Griffin M. Characterization of an FMN-containing cyclohexanone monooxygenase from a cyclohexane-grown Xanthobacter sp. Eur J Biochem. 1989; 181: 199-206.
45. Kostichka K, Thomas SM, Gibson KJ, Nagarajan V, Cheng Q. Cloning and characterization of a gene cluster for cyclododecanone oxidation in Rhodococcus ruber SC1. J Bacteriol. 2001; 183: 6478-6486.
46. Donoghue NA, Norris DB, Trudgill PW. The Purification and properties of Cyclohexanone oxygenase from Nocardia globerula CL1 and Acinetobacter NCIB 9871. Eur J Biochem. 1976; 63: 175-192.
47. Hermann R, Lehmann M, Buchs J. Characterization of gas-liquid mass transfer phenomena in microtiter plates. Biotechnol Bioeng. 2003; 81: 178-186.
48. Doig SD, Pickering SC, Lye GJ, Baganz F. Modelling surface aeration rates in shaken microtitre plates using dimensionless groups. Chem Eng Sci. 2005; 60: 2741-2750.
49. Perry RH, Green DW. Perry's Chemical Engineering Handbook, 6th ed. New York: McGraw Hill; 1997.
50. Micheletti M, Barrett T, Doig SD, Baganz F, Levy MS, Woodley JM, Lye GJ. Fluid mixing in shaken bioreactors: implications for scale-up predications for microlitre-scale microbial and mammalian cell cultures. Chem Eng Sci. 2006; 61: 2939-2949.
51. Zhang H, Lamping SR, Pickering SCR, Lye GJ. Engineering characterisation of a single well from 24-well and 96-well microtitre plates. Biochem Eng J. 2008; 40: 138-149.
52. Marques MPC, Magalhaes S, Cabral JMS, Fernandes P. Characterization of 24-well microtiter plates reactors for a complex multistep bioconversion: from sitosterol to androstenedione. J Biotechnol. 2009; 141: 174-180.
53. Kensy F, Engelbrecht C, Buchs J. Scale-up from microtitre plate to laboratory fermenter: evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha. Microb Cell Fact. 2009; 8: 68