Charge state and adduct reduction in electrospray ionization-mass spectrometry using solvent vapor exposure

Analytical Biochemistry

Volumn 421, Issue 2, 2012, Pages 788-790

  Hopper, Jonathan T S ^a   Sokratous, Kleitos ^a   Oldham, Neil J ^a  

^a UNIVERSITY OF NOTTINGHAM   (United Kingdom)

Author keywords

Noncovalent interactions; Protein complexes; Protein mass spectrometry

Indexed keywords

ADDITIVES; ALKALI METALS; IONS; MASS SPECTROMETRY; ORGANIC SOLVENTS; PROTEINS;

ELECTROSPRAY DROPLETS; ELECTROSPRAY IONIZATION (ESI); ELECTROSPRAY IONIZATION MASS SPECTROMETRY; NON-COVALENT COMPLEXES; NON-COVALENT INTERACTION; PROTEIN COMPLEXES; SOLUTION ADDITIVES; TANDEM MASS SPECTROMETRY;

ELECTROSPRAY IONIZATION;

ACETONITRILE; SOLVENT;

ARTICLE; CHEMICAL INTERACTION; COMPLEX FORMATION; DISSOCIATION; ELECTROSPRAY MASS SPECTROMETRY; PRIORITY JOURNAL; REDUCTION; VAPOR;

EID: 84859568670     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.10.034     Document Type: Article

References (33)

1. J.A. Loo, Studying noncovalent protein complexes by electrospray ionization mass spectrometry, Mass Spectrom. Rev. 16 (1997) 1-23. (Pubitemid 127583217)
2. J.L. Benesch, C.V. Robinson, Mass spectrometry of macromolecular assemblies: preservation and dissociation, Curr. Opin. Struct. Biol. 16 (2006) 245-251.
3. Y. Gordiyenko, H. Videler, M. Zhou, A.R. McKay, P. Fucini, E. Biegel, V. Müller, C.V. Robinson, Mass spectrometry defines the stoichiometry of ribosomal stalk complexes across the phylogenetic tree, Mol. Cell. Proteomics 9 (2010) 1774-1783.
4. J.A. Loo, B. Berhane, C.S. Kaddis, K.M. Wooding, Y. Xie, S.L. Kaufman, I.V. Chernushevich, Electrospray ionization mass spectrometry and ion mobility analysis of the 20S proteasome complex, J. Am. Soc. Mass Spectrom. 16 (2005) 998-1008. (Pubitemid 40828781)
5. H. Prakash, B.T. Kansara, S. Mazumdar, Effects of salts on the charge-state distribution and the structural basis of the most-intense charge-state of the gaseous protein ions produced by electrospray ionization, Int. J. Mass Spectrom. 289 (2010) 84-91.
6. A.K. Frimpong, R.R. Abzatimov, V.N. Uversky, I.A. Kaltashov, Characterization of intrinsically disordered proteins with electrospray ionization mass spectrometry: conformational heterogeneity of a-synuclein, Proteins 78 (2010) 714-722.
7. I.A. Kaltashov, A. Mohimen, Estimates of protein surface areas in solution by electrospray ionization mass spectrometry, Anal. Chem. 77 (2005) 5370-5379. (Pubitemid 41140859)
8. A.T. Lavarone, E.R. Williams, Mechanism of charging and supercharging molecules in electrospray ionization, J. Am. Chem. Soc. 125 (2003) 2319-2327. (Pubitemid 36245725)
9. A.T. Lavarone, J.C. Jurchen, E.R. Williams, Supercharged protein and peptide ions formed by electrospray ionization, Anal. Chem. 73 (2001) 1455-1460. (Pubitemid 32896200)
10. S.H. Lomeli, I.X. Peng, S. Yin, R.R. Loo, J.A. Loo, New reagents for increasing ESI multiple charging of proteins and protein complexes, J. Am. Soc. Mass Spectrom. 21 (2010) 127-131.
11. A. Kharlamova, B.M. Prentice, T.Y. Huang, S.A. McLuckey, Electrospray droplet exposure to gaseous acids for the manipulation of protein charge state distributions, Anal. Chem. 82 (2010) 7422-7429.
12. M. Scalf, M.S. Westphall, J. Krause, S.L. Kaufman, L.M. Smith, Controlling charge states of large ions, Science 283 (1999) 194-197.
13. D. Bagal, E.N. Kitova, L. Liu, A. El-Hawiet, P.D. Schnier, J.S. Klassen, Gas phase stabilization of noncovalent protein complexes formed by electrospray ionization, Anal. Chem. 81 (2009) 7801-7806.
14. R.A. Zubarev, N.L. Kelleher, F.W. McLafferty, Electron capture dissociation of multiply charged protein cations: a nonergodic process, J. Am. Chem. Soc. 120 (1998) 3265-3266.
15. D. Lemaire, G. Marie, L. Serani, O. Laprevote, Stabilization of gas-phase noncovalent macromolecular complexes in electrospray mass spectrometry using aqueous triethylammonium bicarbonate buffer, Anal. Chem. 73 (2001) 1699-1706. (Pubitemid 32862434)
16. F. Halgand, O. Laprevote, Mean charge state and charge state distribution of proteins as structural probes: an electrospray ionisation mass spectrometry study of lysozyme and ribonuclease A, Eur. J. Mass Spectrom. 7 (2001) 433-439. (Pubitemid 33672880)
17. M.I. Catalina, R.H.H. van den Heuvel, E. van Duijn, A.J.R. Heck, Decharging of globular proteins and protein complexes in electrospray, Chem. Eur. J. 11 (2005) 960-968. (Pubitemid 40188534)
18. J. Sun, E.N. Kitova, J.S. Klassen, Method for stabilizing protein-ligand complexes in nanoelectrospray ionization mass spectrometry, Anal. Chem. 79 (2007) 416-425. (Pubitemid 46106105)
19. N. Sun, N. Soya, E.N. Kitova, J.S. Klassen, Nonspecific interactions between proteins and charged biomolecules in electrospray ionization mass spectrometry, J. Am. Soc. Mass Spectrom. 21 (2010) 472-481.
20. E. van Duijn, A. Barendregt, S. Synowsky, C. Versluis, A.J.R. Heck, Chaperonin complexes monitored by ion mobility mass spectrometry, J. Am. Chem. Soc. 131 (2009) 1452-1459.
21. B.E. Winger, K.J. Lightwahl, R.D. Smith, Gas-phase proton-transfer reactions involving multiply charged cytochrome c ions and water under thermal conditions, J. Am. Soc. Mass Spectrom. B (1992) 624-630.
22. S. Nguyen, J.B. Fenn, Gas-phase ions of solute species from charged droplets of solutions, Proc. Natl. Acad. Sci. USA 104 (2007) 1111-1117. (Pubitemid 46183969)
23. Z. Takats, J.M. Wiseman, B. Gologan, R.G. Cooks, Electrosonic spray ionization: a gentle technique for generating folded proteins and protein complexes in the gas phase and for studying ion-molecule reactions at atmospheric pressure, Anal. Chem. 76 (2004) 4050-4058. (Pubitemid 38943677)
24. D. Touboul, M.C. Jecklin, R. Zenobi, Rapid and precise measurements of gas-phase basicity of peptides and proteins at atmospheric pressure by electrosonic spray ionization-mass spectrometry, J. Phys. Chem. B 111 (2007) 11629-11631. (Pubitemid 350006605)
25. D. Touboul, M.C. Jecklin, R. Zenobi, Investigation of deprotonation reactions on globular and denatured proteins at atmospheric pressure by ESSI-MS, J. Am. Soc. Mass Spectrom. 19 (2008) 455-466.
26. R.R.O. Loo, H.R. Udseth, R.D. Smith, Evidence of charge inversion in the reaction of singly charged anions with multiply charged macroions, J. Phys. Chem. 95 (1991) 6412-6415.
27. R.R.O. Loo, H.R. Udseth, R.D. Smith, A new approach for the study of gas-phase ion-ion reactions using electrospray ionization, J. Am. Soc. Mass Spectrom. 3 (1992) 695-705.
28. Q. Zhao, M.W. Soyk, G.M. Schieffer, K. Fuhrer, M.M. Gonin, R.S. Houk, E.R. Badman, An ion trap-ion mobility-time of flight mass spectrometer with three ion sources for ion/ion reactions, J. Am. Soc. Mass Spectrom. 20 (2009) 1549-1561.
29. Q. Zhao, G.M. Schieffer, M.W. Soyk, T.J. Anderson, R.S. Houk, E.R. Badman, Effects of ion/ion proton transfer reactions on conformation of gas-phase cytochrome c ions, J. Am. Soc. Mass Spectrom. 21 (2010) 1208-1217.
30. S.M. Clark, L. Konermann, Determination of ligand-protein dissociation constants by electrospray mass spectrometry-based diffusion measurements, Anal. Chem. 76 (2004) 7077-7083. (Pubitemid 39600883)
31. K. Pagel, S.J. Hyung, B.T. Ruotolo, C.V. Robinson, Alternate dissociation pathways identified in charge-reduced protein complex ions, Anal. Chem. 82 (2010) 5363-5372.
32. D. Rauh, G. Klebe, J. Sturzebecher, M.T. Stubbs, ZZ made EZ: influence of inhibitor configuration on enzyme selectivity, J. Mol. Biol. 330 (2003) 761-770.
33. J.T. S Hopper, N.J. Oldham, Alkali metal cation-induced destabilization of gas-phase protein-ligand complexes: consequences and prevention, Anal. Chem. 83 (2011) 7472-7479.