Dual-label time-resolved fluoroimmunoassay for simultaneous quantification of haptoglobin and C-reactive protein in meat juice from pigs

Canadian Journal of Veterinary Research

Volumn 76, Issue 2, 2012, Pages 136-142

  Gutiérrez, Ana M ^a   Cerón, José J ^a   Marsilla, Blas A ^b   Parra, María D ^c   Martinez Subiela, Silvia ^a  

^a UNIVERSITY OF MURCIA   (Spain)

^b Sanitary and Consumption Office ^*  (Spain)

^c Health Division of Lorca Town Council   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ACUTE PHASE PROTEIN; C REACTIVE PROTEIN; EUROPIUM; HAPTOGLOBIN; LANTHANIDE; SAMARIUM;

ACCURACY; ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; FLUOROIMMUNOASSAY; LIMIT OF DETECTION; LIMIT OF QUANTITATION; MALE; MEAT; NONHUMAN; SCREENING TEST; SWINE; TIME RESOLVED IMMUNOFLUOROMETRY; TISSUE LEVEL;

ANIMALS; C-REACTIVE PROTEIN; EUROPIUM; FLUOROIMMUNOASSAY; FOOD ANALYSIS; HAPTOGLOBINS; LIMIT OF DETECTION; MALE; MEAT; REPRODUCIBILITY OF RESULTS; SAMARIUM; STATISTICS, NONPARAMETRIC; SWINE;

ANIMALIA; SUIDAE; SUS;

EID: 84859471409     PISSN: 08309000     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Millet S, Cox E, Buyse J, Goddeeris BM, Janssens GP. Immunocompetence of fattening pigs fed organic versus conventional diets in organic versus conventional housing. Vet J 2005;169: 293-299.
2. Heo J, Kattesh HG, Roberts MP, Morrow JL, Dailey JW, Saxton AM. Hepatic corticosteroid-binding globulin (CBG), messenger RNA expression and plasma CBG concentration in young pigs in response to heat and social stress. J Anim Sci 2005;83:208-215.
3. Gómez-Laguna J, Salguero FJ, Pallarés FJ, et al. Acute phase response in porcine reproductive and respiratory syndrome virus infection. Comp Immunol Microbiol Infect Dis 2010;33:e51-e58.
4. Skovgaard K, Mortensen S, Boye M, et al. Rapid and widely disseminated acute phase protein response after experimental bacterial infection of pigs. Vet Res 2009;40:23. Epub 2009 Feb 24.
5. Sánchez-Cordón PJ, Cerón JJ, Núñez A, et al. Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses. Am J Vet Res 2007;68:772-777.
6. Toussaint MJM, Van Ederen AM, Gruys E. Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Int 1995;5:149-157.
7. Eckersall PD. Acute phase proteins as markers of infection and inflammation: Monitoring animal health, animal welfare and food safety. Ir Vet J 2000;53:307-311.
8. Kricka LJ. Multianalyte testing. Clin Chem 1992;38:327-328.
9. Sheng SL, Wang Q, Huang G, Yu B, Qin WX. Simultaneous determination of alpha-fetoprotein immune complexes and alpha-fetoprotein concentration in hepatocellular carcinoma using dual-label time-resolved immunofluorometric assays. J Clin Lab Anal 2009;23:179-185.
10. Kimura H, Mukaida M, Wang G, Yuan J, Matsumoto K. Duallabel time-resolved fluoroimmunoassay of psychopharmaceuticals and stimulants in serum. Forensic Sci Int 2000;113:345-351.
11. Huang B, Zhang J, Ma Z, Zhang Y. Simultaneous detection of aflatoxin B1 and ochratoxin A by the dual-label time-resolved fluoroimmunoassay. Wei Sheng Yan Jiu 2009;38:385-388.
12. 1-antichymotrypsin complex together with free or total PSA. Clin Chem 2003;49:97-103.
13. Parkkila AK, Parkkila S, Serlo W, Reunanen M, Vierjoki T, Rajaniemi H. A competitive dual-label time-resolved immunofluorometric assay for simultaneous detection of carbonic anhydrase I and II in cerebrospinal fluid. Clin Chim Acta 1994;230: 81-89.
14. Ankelo M, Westerlund A, Blomberg K, Knip M, Ilonen J, Hinkkanen AE. Time-resolved immunofluorometric dual-label assay for simultaneous detection of autoantibodies to GAD65 and IA-2 in children with type 1 diabetes. Clin Chem 2007;53: 72-79.
15. Diamandis EP. Immunoassays with time-resolved fluorescence spectroscopy: Principles and applications. Clin Biochem 1988;21:139-150.
16. Erkens JH, Dieleman SJ, Dressendörfer RA, Strasburger CJ. A time-resolved fluoroimmunoassay for cortisol in unextracted bovine plasma or serum with optimized procedures to eliminate steroid binding protein interference and to minimize non-specific streptavidin-europium binding. J Steroid Biochem Mol Biol 1998;67:153-161.
17. Parra MD, Bernal LJ, Cerón JJ. Cortisol and free thyroxine determination by time-resolved fluorometry in canine serum. Can J Vet Res 2004;68:98-104.
18. Tuomola M, Vainio J, Lövgren T. Rapid time-resolved immunofluorometric assay for the measurement of creatine kinase in serum and whole blood samples. J Agric Food Chem 2002;50: 6659-6662.
19. Parra MD, Fuentes P, Tecles F, et al. Porcine acute phase protein concentrations in different diseases in field conditions. J Vet Med B Infect Dis Vet Public Health 2006;53:488-493.
20. Gutiérrez AM, Martínez-Subiela S, Montes A, Parra MD, Cerón JJ. C-reactive protein measurements in meat juice of pigs. Vet Immunol Immunopathol 2008;122:250-255.
21. Gutiérrez AM, Martínez-Subiela S, Cerón JJ. Evaluation of an immunoassay for determination of haptoglobin concentration in various biological specimens from swine. Am J Vet Res 2009;70:691-696.
22. US Department of Health and Human Services, Food and Drug Administration, Center for Drug Evaluation and Research and Center for Veterinary Medicine. Guidance for Industry. Bioanalytical Method Validation. Rockville, Maryland: FDA, US Department of Health and Human Services, May 2001. Pages: 5-6. Available from http://www.fda.gov/downloads/Drugs/ GuidanceComplianceRegulatoryInformation/Guidances/ ucm070107.pdf Last accessed February 9, 2012.
23. Zhang J, Gao L, Zhou B, Zhu L, Zhang Y, Huang B. Simultaneous detection of deoxynivalenol and zearalenone by dual-label time-resolved fluorescence immunoassay. J Sci Food Agric 2011;91:193-197.
24. Lövgren T, Meriö L, Mitrunen K, et al. One-step all-in-one dry reagent immunoassays with fluorescent europium chelate label and time-resolved fluorometry. Clin Chem 1996;42:1196-1201.
25. Gutiérrez AM, Martínez-Subiela S, Soler L, Pallarés FJ, Cerón JJ. Use of saliva for haptoglobin and C-reactive protein quantifications in porcine respiratory and reproductive syndrome affected pigs in field conditions. Vet Immunol Immunopathol 2009;132:218-223.
26. Kjelgaard-Hasen M, Jacobser S. Assay validation and diagnostic applications of major acute-phase protein testing in companion animals. Clin Lab Med 2011;31:51-70. Epub 2010 Dec 3.
27. Piñeiro M, Gymnich S, Knura S, Piñeiro C, Petersen B. Meat juice: An alternative matrix for assessing animal health by measuring acute phase proteins. Correlations of pig-MAP and haptoglobin concentrations in pig meat juice and plasma. Res Vet Sci 2009;87:273-276