Efficient production and characterization of recombinant human NELL1 protein in human embryonic kidney 293-F cells

Molecular Biotechnology

Volumn 51, Issue 1, 2012, Pages 58-66

  Hasebe, Ai ^a   Tashima, Hiroki ^a   Ide, Teruhiko ^a   Iijima, Masumi ^a   Yoshimoto, Nobuo ^a   Ting, Kang ^b   Kuroda, Shun'Ichi ^a   Niimi, Tomoaki ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bone regeneration; Extracellular matrix; NELL 1; Osteoblast differentiation

Indexed keywords

BONE FORMATION; BONE REGENERATION; CELL EXPRESSION; CULTURE DISHES; CULTURE MEDIUM; DEAD CELLS; EFFICIENT PRODUCTION; EXTRACELLULAR MATRICES; EXTRACELLULAR MATRIX COMPONENTS; GLASS COVERS; GLYCOSYLATED; HUMAN EMBRYONIC KIDNEYS; MEDIATED SIGNALING PATHWAYS; MULTIMERS; NELL-1; NOVEL TECHNIQUES; OSTEOBLAST DIFFERENTIATION; OSTEOBLASTIC CELLS; OSTEOGENIC; OSTEOGENIC DIFFERENTIATION; PROTEIN PRODUCTION; SERUM-FREE MEDIUM; STABLE CELL LINES; TRANSFECTED CELLS;

AFFINITY CHROMATOGRAPHY; BONE; CELLS; PURIFICATION; RECOMBINANT PROTEINS;

CELL CULTURE;

ANTIBIOTIC G 418; GELATIN; GLASS; HEXAHISTIDINE; NICKEL COMPLEX; POLYMER; PROTEIN NELL1; PROTEINASE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; BONE REGENERATION; CARBOXY TERMINAL SEQUENCE; CELL GROWTH; CELL LINE; CULTURE MEDIUM; EMBRYO CELL; EXTRACELLULAR MATRIX; GENETIC TRANSFECTION; GLYCOSYLATION; HUMAN; HUMAN CELL; KIDNEY CELL; MESENCHYME CELL; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN ANALYSIS; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION;

ANIMALS; BIOLOGICAL MARKERS; BIOTECHNOLOGY; CELL SEPARATION; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EXTRACELLULAR MATRIX; GENETIC VECTORS; GLYCOSYLATION; HEK293 CELLS; HUMANS; MICE; NERVE TISSUE PROTEINS; OSTEOGENESIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TIME FACTORS;

HEXAPODA;

EID: 84859430256     PISSN: 10736085     EISSN: 15590305     Source Type: Journal    
DOI: 10.1007/s12033-011-9440-4     Document Type: Article

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