Preliminary study of the cellular characteristics of primary bronchial fibroblasts in patients with asthma: Expression of α-smooth muscle actin, fibronectin containing extra type III domain a, and smoothelin

Journal of Investigational Allergology and Clinical Immunology

Volumn 22, Issue 1, 2012, Pages 20-27

  Nam, Y H ^a,b   Lee, Soo Keol ^a,b   Sammut, D ^c   Davies, D E ^c   Howarth, P H ^c  

^a Dong A University   (South Korea)

^b Dong A University College of Medicine   (South Korea)

^c UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Asthma; Fibroblast; Fibronectins; Smooth muscle actin

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; BETA 2 ADRENERGIC RECEPTOR STIMULATING AGENT; CORTICOSTEROID; FIBRONECTIN; LEUKOTRIENE RECEPTOR BLOCKING AGENT; LONG ACTING DRUG; SMOOTHELIN;

ADULT; ARTICLE; ASTHMA; CASE REPORT; CELL COUNT; CELL STRUCTURE; DISEASE SEVERITY; EMBRYO CELL; EXTRACELLULAR MATRIX; FIBROBLAST; FLUORESCENCE MICROSCOPY; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; INCUBATION TIME; MALE; PRIMARY BRONCHIAL FIBROBLAST; PROTEIN DOMAIN; PROTEIN EXPRESSION; SYMPTOM;

ACTINS; ADULT; ASTHMA; BRONCHI; CELLS, CULTURED; CYTOSKELETAL PROTEINS; FIBROBLASTS; FIBRONECTINS; HUMANS; MALE; MIDDLE AGED; MUSCLE PROTEINS; MUSCLE, SMOOTH; MYOFIBROBLASTS;

EID: 84859205419     PISSN: 10189068     EISSN: 16980808     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. Tomasek JJ, Gabbiani G, Hinz B, Chaponnier C, Brown RA. Myofi broblasts and mechano-regulation of connective tissue remodeling. Nat Rev Mol Cell Biol. 2002;3:349-63.
2. Singh SR, Hall IP. Airway myofi broblasts and their relationship with airway myocytes and fi broblasts. Proc Am Thorac Soc. 2008;5:127-32.
3. Michalik M, Pierzchailska M, Legutko A, Ura M, Ostaszewska A, Soja J, Sanak M. Asthmatic bronchial fi broblasts demonstrate enhanced potential to differentiate into myofi broblasts in culture. Med Sci Monit. 2009;15:194-201.
4. Pankow R, Yamada KM. Fibronectin at a glance. J Cell Sci. 2002;115:3861-3.
5. Serini G, Bochaton-Piallat ML, Ropraz P, Geninoz A, Borsi L, Zardi L, Gabbiani G. The fi bronectin domain ED-A is crucial for myofi broblastic phenotype induction by transforming growth factor-beta 1. J Cell Biol. 1998;142:873-81.
6. Muro AF, Chauhan AK, Gajovic S, Iaconcig A, Porro F, Stanta G, Baralle FE. Regulated splicing of the fi bronectin EDA exon is essential for proper skin wound healing and normal lifespan. J Cell Biol. 2003;162:149-60.
7. Van der Loop FT, Gabbiani G, Kohnen G, Ramaekers FC, van Eys GJ. Differentiation of smooth muscle cells in human blood vessels as defi ned by smoothelin, a novel marker for the contractile phenotype. Arterioscler Thromb Vasc Biol. 1997;17:665-71.
8. Christen T, Verin V, Bochaton-Piallat M, Popowski Y, Ramaekers F, Debruyne P, Camenzind E, van Eys G, Gabbiani G. Mechanisms of neointima formation and remodeling in the porcine coronary artery. Circulation. 2001;103:882-8.
9. Van der Loop FT, Schaart G, Timmer ED, Pamaekers FC, van Eys G. Smoothelin, a novel cytoskeletal protein specifi c for smooth muscle cells. J Cell Biol. 1996;134:401-11.
10. Richter A, Puddicombe SM, Lordan JL, Bucchieri F, Wilson SJ, Djukanovic R, Dent G, Holgate ST, Davies DE. The contribution of interleukin (IL)-4 and IL-13 to the epithelial-mesenchymal trophic unit in asthma. Am J Respir Cell Mol Biol. 2001;25:385-91.
11. Global Strategy for Asthma Management and Prevention. Global Initiative for Asthma (GINA), 2006. Available at: http:// www.ginasthma.org
12. Hanley NA, Hagan DM, Clement-Jones M, Ball SG, Strachan T, Salas-Cortes L, McElready K, Lindsay S, Robson S, Bullen P, Oster H, Wilson DI. SRY, SOX9, and DAX1 expression patterns during human sex determination and gonadal development. Mech Dev. 2000;91:403-7.
13. Gabbiani G. The biology of the myofi broblast. Kidney Int. 1992;41:530-2.
14. Abe R, Donnelly SC, Peng T, Bucala R, Metz CN. Peripheral blood fi brocytes: differentiation pathway and migration to wound sites. J Immunol. 2001;166:7556-62.
15. Nihlberg K, Larsen K, Hultgardh-Nilsson A, Malmstrom A, Bjermer L, Westergren-Thorsson G. Tissue fi brocytes in patients with mild asthma: a possible link to thickness of reticular basement membrane? Respir Res. 2006;29:7:50.
16. Schmidt M, Sun G, Stacey MA, Mori L, Mattoli S. Identifi cation of circulating fi brocytes as precursors of bronchial myofi broblasts in asthma. J Immunol. 2003;171:1380-9.
17. Bucala R, Spiegel LA, Chesney J, Hogan M, Cerami A. Circulating fi brocytes defi ne a new leukocyte subpopulation that mediates tissue repair. Mol Med. 1994;1:71-81.
18. Serini G, Gabbiani G. Mechanisms of myofi broblast activity and phenotypic modulation. Exp Cell Res. 1999;250:273-83.
19. Powell DW, Miffl in RC, Valentich JD, Crowe SE, Saada JI, West AB. Myofi broblast. I. Paracrine cells important in health and disease. Am J Physiol. 1999;277:1-9.
20. Schmid P, Itin P, Cherry G, Bi C, Cox DA. Enhanced expression of transforming growth factor-ß type I and type II receptors in wound healing tissue and hypertrophied scar. Am J Pathol. 1998;152:485-93
21. Kim SJ, Angel P, Lafyatis R, Hattori K, Kim KY, Sporn MB, Karin M, Roberts AB. Autoinduction of transforming growth factor beta 1 is mediated by the AP-a complex. Mol Cell Biol. 1990;10:1492-7.
22. Hinz B, Mastrangelo D, Iselin CE, Chaponnier C, Gabbiani G. Mechanical tension controls granulation tissue contractile activity and myofi broblast differentiation. Am J Pathol. 2001;159:1009-20.
23. Masur SK, Dewal HS, Dinh TT, Erenburg I, Petridou S. Myofi broblasts differentiate from fi broblasts when plated at low density. Proc Natl Acad Sci USA. 1996;93:4219-23.
24. Liu M, Tanswell K, Post M. Mechanical force-induced signal transduction in lung cells. Am J Physiol Lung Cell Mol Physiol. 1999;277:667-83.
25. Dekkers BG, Bos ST, Gosens R, Halayko AJ, Zaagsma J, Meurs H. The integrin-blocking peptide RGDS inhibits airway smooth muscle remodeling in a guinea pig model of allergic asthma. Am J Respir Crit Care Med. 2010;181:556-65.
26. Freyer AM, Johnson SR, Hall IP. Effects of growth factors and extracellular matrix on survival of human airway smooth muscle cells. Am J Respir Cell Mol Biol. 2001;25:569-76.
27. Nguyen TT, Ward JP, Hirst SJ. ß1-Integrins mediate enhancement of airway smooth muscle proliferation by collagen and fi bronectin. Am J Respir Crit Care Med. 2005;171:217-23.
28. Hernnas J, Nettelbladt O, Bjermer L, Sarnstrand B, Malmstrom A, Hallgren R. Alveolar accumulation of fi bronectin and hyaluronan precedes bleomycin-induced pulmonary fi brosis in the rat. Eur Respir J. 1992;5:404-10.
29. Muro AF, Caputi M, Pariyarath R, Pagani F, Buratti E, Baralle FE. Regulation of fi bronectin EDA exon alternative slicing: possible role of RNA secondary structure for enhancer display. Mol Cell Biol. 1999;19:2657-71.
30. Buratti E, Baralle FE. Infl uence of RNA secondary structure on the pre-mRNA splicing process. Mol Cell Biol. 2004;24:10505-14.
31. Burgess HA, Daugherty LE, Thatcher TH, Lakatos HF, Ray DM, Redonnet M, Phipps RP, Sime PJ. PPAR-RRagonists inhibit TGF-ß induced pulmonary myofi broblast differentiation and collagen production: implications for therapy of lung fi brosis. Am J Physiol Lung Cell Mol Physiol. 2005;288:1146-53.
32. Muro AF, Moretti FA, Moore BB, Yan M, Atrasz RG, Wilke CA, Flaherty KR, Martinez FJ, Tsui JL, Sheppard D, Baralle FE, Toews GB, White ES. An essential role for fi bronectin extra type III domain A in pulmonary fi brosis. Am J Respir Crit Care Med. 2008;177:638-45.
33. Kohan M, Muro AF, Bader R, Berkman N. The extra domain of fi bronectin is essential for allergen-induced airway fi brosis and hyperresponsiveness in mice. J Allergy Clin Immunol. 2011;127:439-46.
34. Wenzel SC, Balzar S. Myofi broblast or smooth muscle: do in vitro systems adequately replicate tissue smooth muscle? Am J Respir Crit Care Med. 2006;174:364-5.
35. Grinnell F. Fibroblast-collagen-matrix contraction: growth factor signaling and mechanical loading. Trends Cell Biol. 2000;10:362-5.