The integrin-blocking peptide RGDS inhibits airway smooth muscle remodeling in a guinea pig model of allergic asthma

American Journal of Respiratory and Critical Care Medicine

Volumn 181, Issue 6, 2010, Pages 556-565

  Dekkers, Bart G J ^a   Bos, I Sophie T ^a   Gosens, Reinoud ^a   Halayko, Andrew J ^b   Zaagsma, Johan ^a   Meurs, Herman ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^b UNIVERSITY OF MANITOBA   (Canada)

Author keywords

Airway remodeling; Airway smooth muscle contractility; Airway smooth muscle hyperplasia; Extracellular matrix; Integrin

Indexed keywords

ALLERGEN; ALPHA ACTIN; ARGINYLGLYCYLASPARTYLSERINE; COLLAGEN TYPE 1; CYCLINE; FIBRONECTIN; GLYCYLARGINYLALANYLASPARTYLSERYLPROLINE; MYOSIN HEAVY CHAIN; PEPTIDE DERIVATIVE; PLATELET DERIVED GROWTH FACTOR; UNCLASSIFIED DRUG; VERY LATE ACTIVATION ANTIGEN 5;

AIRWAY; ALLERGIC ASTHMA; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL MATURATION; CELL PROLIFERATION; CONTROLLED STUDY; EOSINOPHIL; FIBROSIS; GUINEA PIG; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HYPERPLASIA; IN VITRO STUDY; INFLAMMATION; MATURATION; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SERUM; SMOOTH MUSCLE CONTRACTILITY; SMOOTH MUSCLE FIBER;

ADMINISTRATION, TOPICAL; AIRWAY REMODELING; ANIMALS; ANTIBODIES; ASTHMA; BIOLOGICAL MARKERS; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; DISEASE MODELS, ANIMAL; FIBROSIS; GUINEA PIGS; HUMANS; INFLAMMATION; MALE; MUSCLE CONTRACTION; MUSCLE, SMOOTH; OLIGOPEPTIDES; PLATELET AGGREGATION INHIBITORS; PROLIFERATING CELL NUCLEAR ANTIGEN;

EID: 77749324374     PISSN: 1073449X     EISSN: 15354970     Source Type: Journal    
DOI: 10.1164/rccm.200907-1065OC     Document Type: Article

References (55)

1. Bousquet J, Jeffery PK, Busse WW, Johnson M, Vignola AM. Asthma: from bronchoconstriction to airways inflammation and remodeling. Am J Respir Crit Care Med 2000;161:1720-1745.
2. Davies DE, Wicks J, Powell RM, Puddicombe SM, Holgate ST. Airway remodeling in asthma: new insights. J Allergy Clin Immunol 2003;111: 215-225.
3. Postma DS, Kerstjens HA. Characteristics of airway hyper-responsiveness in asthma and chronic obstructive pulmonary disease. Am J Respir Crit Care Med 1998;158:S187-S192.
4. An SS, Bai TR, Bates JH, Black JL, Brown RH, Brusasco V, Chitano P, Deng L, Dowell M, Eidelman DH, et al. Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma. Eur Respir J 2007;29:834-860.
5. Dunnill MS, Massarella GR, Anderson JA. A comparison of the quantitative anatomy of the bronchi in normal subjects, in status asthmaticus, in chronic bronchitis, and in emphysema. Thorax 1969;24:176-179.
6. Parameswaran K, Willems-Widyastuti A, Alagappan VK, Radford K, Kranenburg AR, Sharma HS. Role of extracellular matrix and its regulators in human airway smooth muscle biology. Cell Biochem Biophys 2006;44:139-146.
7. Fernandes DJ, Bonacci JV, Stewart AG. Extracellular matrix, integrins, and mesenchymal cell function in the airways. Curr Drug Targets 2006;7:567-577.
8. Bai TR, Cooper J, Koelmeyer T, Pare PD, Weir TD. The effect of age and duration of disease on airway structure in fatal asthma. Am J Respir Crit Care Med 2000;162:663-669.
9. Thomson RJ, Schellenberg RR. Increased amount of airway smooth muscle does not account for excessive bronchoconstriction in asthma. Can Respir J 1998;5:61-62.
10. Araujo BB, Dolhnikoff M, Silva LF, Elliot J, Lindeman JH, Ferreira DS, Mulder A, Gomes HA, Fernezlian SM, James A, et al. Extracellular matrix components and regulators in the airway smooth muscle in asthma. Eur Respir J 2008;32:61-69.
11. Dekkers BG, Schaafsma D, Nelemans SA, Zaagsma J, Meurs H. Extracellular matrix proteins differentially regulate airway smooth muscle phenotype and function. Am J Physiol Lung Cell Mol Physiol 2007;292:L1405-L1413.
12. Hirst SJ, Twort CH, Lee TH. Differential effects of extracellular matrix proteins on human airway smooth muscle cell proliferation and phenotype. Am J Respir Cell Mol Biol 2000;23:335-344.
13. Johnson PR, Burgess JK, Underwood PA, Au W, Poniris MH, Tamm M, Ge Q, Roth M, Black JL. Extracellular matrix proteins modulate asthmatic airway smooth muscle cell proliferation via an autocrine mechanism. J Allergy Clin Immunol 2004;113:690-696.
14. Hynes RO. Integrins: bidirectional, allosteric signaling machines. Cell 2002;110:673-687.
15. Nguyen TT, Ward JP, Hirst SJ. b1-Integrins mediate enhancement of airway smooth muscle proliferation by collagen and fibronectin. Am J Respir Crit Care Med 2005;171:217-223.
16. Moir LM, Johnson PR, Burgess JK, Black JL. Integrins mediate asthmatic airway smooth muscle cell proliferation. Presented at the Thoracic Society of Australia and New Zealand 2005 Annual Conference Meeting, March 18-23, 2005, Perth, Australia.
17. Ma X, Cheng Z, Kong H, Wang Y, Unruh H, Stephens NL, Laviolette M. Changes in biophysical and biochemical properties of single bronchial smooth muscle cells from asthmatic subjects. Am J Physiol Lung Cell Mol Physiol 2002;283:L1181-L1189.
18. Leguillette R, Laviolette M, Bergeron C, Zitouni NB, Kogut P, Solway J, Kashmar L, Hamid Q, Lauzon AM. Myosin, transgelin, and myosin light chain kinase: expression and function in asthma. Am J Respir Crit Care Med 2008;179:194-204.
19. Ma X, Wang Y, Stephens NL. Serum deprivation induces a unique hypercontractile phenotype of cultured smooth muscle cells. Am J Physiol 1998;274:C1206-C1214.
20. Halayko AJ, Salari H, Ma X, Stephens NL. Markers of airway smooth muscle cell phenotype. Am J Physiol 1996;270:L1040-L1051.
21. Schaafsma D, McNeill KD, Stelmack GL, Gosens R, Baarsma HA, Dekkers BG, Frohwerk E, Penninks JM, Sharma P, Ens KM, et al. Insulin increases the expression of contractile phenotypic markers in airway smooth muscle. Am J Physiol Cell Physiol 2007;293:C429-C439.
22. Tran T, McNeill KD, Gerthoffer WT, Unruh H, Halayko AJ. Endogenous laminin is required for human airway smooth muscle cell maturation. Respir Res 2006;7:117.
23. Dekkers BG, Schaafsma D, Tran T, Zaagsma J, Meurs H. Insulininduced laminin expression promotes a hypercontractile airway smooth muscle phenotype. Am J Respir Cell Mol Biol 2009;41:494-504.
24. Plow EF, Haas TA, Zhang L, Loftus J, Smith JW. Ligand binding to integrins. J Biol Chem 2000;275:21785-21788.
25. Aumailley M, Gerl M, Sonnenberg A, Deutzmann R, Timpl R. Identification of the Arg-Gly-Asp sequence in laminin A chain as a latent cell-binding site being exposed in fragment P1. FEBS Lett 1990;262:82-86.
26. Dekkers BG, Bos IS, Gosens R, Halayko AJ, Zaagsma J, Meurs H. Inhibition of airway smooth muscle remodeling in an animal model of chronic asthma by the integrin-blocking peptide RGDS [abstract]. Am J Respir Crit Care Med 2009;179:A5600.
27. Meurs H, Santing RE, Remie R, van der Mark TW, Westerhof FJ, Zuidhof AB, Bos IS, Zaagsma J. A guinea pig model of acute and chronic asthma using permanently instrumented and unrestrained animals. Nat Protoc 2006;1:840-847.
28. Bos IS, Gosens R, Zuidhof AB, Schaafsma D, Halayko AJ, Meurs H, Zaagsma J. Inhibition of allergen-induced airway remodelling by tiotropium and budesonide: a comparison. Eur Respir J 2007;30:653-661.
29. Gosens R, Bos IS, Zaagsma J, Meurs H. Protective effects of tiotropium bromide in the progression of airway smooth muscle remodeling. Am J Respir Crit Care Med 2005;171:1096-1102.
30. Erjefalt JS, Sundler F, Persson CG. Eosinophils, neutrophils, and venular gaps in the airway mucosa at epithelial removal-restitution. Am J Respir Crit Care Med 1996;153:1666-1674.
31. Gosens R, Dueck G, Gerthoffer WT, Unruh H, Zaagsma J, Meurs H, Halayko AJ. p42/p44 MAP kinase activation is localized to caveolae-free membrane domains in airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 2007;292:L1163-L1172.
32. Gosens R, Stelmack GL, Dueck G, McNeill KD, Yamasaki A, Gerthoffer WT, Unruh H, Gounni AS, Zaagsma J, Halayko AJ. Role of caveolin-1 in p42/p44 MAP kinase activation and proliferation of human airway smooth muscle. Am J Physiol Lung Cell Mol Physiol 2006;291:L523-L534.
33. Moldovan GL, Pfander B, Jentsch S. PCNA, the maestro of the replication fork. Cell 2007;129:665-679.
34. 1 integrins mediate enhancement of human airway smooth muscle cytokine secretion by fibronectin and type I collagen. J Immunol 2005;174:2258-2264.
35. Chan V, Burgess JK, Ratoff JC, O'Connor BJ, Greenough A, Lee TH, Hirst SJ. Extracellular matrix regulates enhanced eotaxin expression in asthmatic airway smooth muscle cells. Am J Respir Crit Care Med 2006;174:379-385.
36. Bazan-Socha S, Bukiej A, Marcinkiewicz C, Musial J. Integrins in pulmonary inflammatory diseases. Curr Pharm Des 2005;11:893-901.
37. Jeffery PK. Remodeling in asthma and chronic obstructive lung disease. Am J Respir Crit Care Med 2001;164:S28-S38.
38. Postma DS, Timens W. Remodeling in asthma and chronic obstructive pulmonary disease. Proc Am Thorac Soc 2006;3:434-439.
39. Ebina M, Takahashi T, Chiba T, Motomiya M. Cellular hypertrophy and hyperplasia of airway smooth muscles underlying bronchial asthma: a 3-D morphometric study. Am Rev Respir Dis 1993;148: 720-726.
40. Bonacci JV, Harris T, Stewart AG. Impact of extracellular matrix and strain on proliferation of bovine airway smooth muscle. Clin Exp Pharmacol Physiol 2003;30:324-328.
41. Freyer AM, Johnson SR, Hall IP. Effects of growth factors and extracellular matrix on survival of human airway smooth muscle cells. Am J Respir Cell Mol Biol 2001;25:569-576.
42. Ichii T, Koyama H, Tanaka S, Kim S, Shioi A, Okuno Y, Raines EW, Iwao H, Otani S, Nishizawa Y. Fibrillar collagen specifically regulates human vascular smooth muscle cell genes involved in cellular responses and the pericellular matrix environment. Circ Res 2001;88: 460-467.
43. 1 on human airway smooth muscle. J Allergy Clin Immunol 2008;121: 1034-1039.
44. Cockcroft DW, Davis BE. Mechanisms of airway hyperresponsiveness. J Allergy Clin Immunol 2006;118:551-559.
45. Hirst SJ, Martin JG, Bonacci JV, Chan V, Fixman ED, Hamid QA, Herszberg B, Lavoie JP, McVicker CG, Moir LM, et al. Proliferative aspects of airway smooth muscle. J Allergy Clin Immunol 2004;114: S2-S17.
46. Meurs H, Gosens R, Zaagsma J. Airway hyperresponsiveness in asthma: lessons from in vitro model systems and animal models. Eur Respir J 2008;32:487-502.
47. Davis GE, Bayless KJ, Davis MJ, Meininger GA. Regulation of tissue injury responses by the exposure of matricryptic sites within extracellular matrix molecules. Am J Pathol 2000;156:1489-1498.
48. Black JL, Burgess JK, Johnson PR. Airway smooth muscle - its relationship to the extracellular matrix. Respir Physiol Neurobiol 2003; 137:339-346.
49. Hirst SJ, Walker TR, Chilvers ER. Phenotypic diversity and molecular mechanisms of airway smooth muscle proliferation in asthma. Eur Respir J 2000;16:159-177.
50. Tran T, Halayko AJ. Extracellular matrix and airway smooth muscle interactions: a target for modulating airway wall remodelling and hyperresponsiveness? Can J Physiol Pharmacol 2007;85:666-671.
51. Ponath PD, Qin S, Ringler DJ, Clark-Lewis I, Wang J, Kassam N, Smith H, Shi X, Gonzalo JA, Newman W, et al. Cloning of the human eosinophil chemoattractant, eotaxin: expression, receptor binding, and functional properties suggest a mechanism for the selective recruitment of eosinophils. J Clin Invest 1996;97:604-612.
52. McMillan SJ, Xanthou G, Lloyd CM. Manipulation of allergen-induced airway remodeling by treatment with anti-TGF-β antibody: effect on the Smad signaling pathway. J Immunol 2005;174:5774-5780.
53. Woodside DG, Vanderslice P. Cell adhesion antagonists: therapeutic potential in asthma and chronic obstructive pulmonary disease. Bio Drugs 2008;22:85-100.
54. Lambert RK, Wiggs BR, Kuwano K, Hogg JC, Pare PD. Functional significance of increased airway smooth muscle in asthma and COPD. J Appl Physiol 1993;74:2771-2781.
55. Oliver MN, Fabry B, Marinkovic A, Mijailovich SM, Butler JP, Fredberg JJ. Airway hyperresponsiveness, remodeling, and smooth muscle mass: right answer, wrong reason? Am J Respir Cell Mol Biol 2007;37:264-272.