Differences in the Mechanisms of Proapoptotic BH3 Proteins Binding to Bcl-XL and Bcl-2 Quantified in Live MCF-7 Cells

Molecular Cell

Volumn 45, Issue 6, 2012, Pages 754-763

  Aranovich, Alexander ^a   Liu, Qian ^a   Collins, Tony ^a   Geng, Fei ^a   Dixit, Sudeepa ^a   Leber, Brian ^a   Andrews, David W A ^a  

^a MCMASTER UNIVERSITY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

4 [4 (4' CHLORO 2 BIPHENYLYLMETHYL) 1 PIPERAZINYL] N [4 [3 DIMETHYLAMINO 1 (PHENYLTHIOMETHYL)PROPYLAMINO] 3 NITROBENZENESULFONYL]BENZAMIDE; BH3 PROTEIN; BIM PROTEIN; PROTEIN BAD; PROTEIN BCL 2; PROTEIN BCL XL; PROTEIN BID;

ANIMAL CELL; APOPTOSIS; ARTICLE; CANCER RESISTANCE; CARCINOGENESIS; CONTROLLED STUDY; CYTOPLASM; DRUG SELECTIVITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE MUTATION; IN VITRO STUDY; MITOCHONDRION; MOUSE; NONHUMAN; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN SECRETION;

AMINO ACID SEQUENCE; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; BACTERIAL PROTEINS; BCL-ASSOCIATED DEATH PROTEIN; BCL-X PROTEIN; BH3 INTERACTING DOMAIN DEATH AGONIST PROTEIN; BIPHENYL COMPOUNDS; BREAST NEOPLASMS; CELL LINE, TUMOR; FEMALE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; LUMINESCENT PROTEINS; MEMBRANE PROTEINS; MITOCHONDRIA; MOLECULAR SEQUENCE DATA; NITROPHENOLS; PIPERAZINES; PROTEIN INTERACTION MAPS; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-BCL-2; SULFONAMIDES;

EID: 84859056673     PISSN: 10972765     EISSN: 10974164     Source Type: Journal    
DOI: 10.1016/j.molcel.2012.01.030     Document Type: Article

References (34)

1. Billen L.P., Kokoski C.L., Lovell J.F., Leber B., Andrews D.W. Bcl-XL inhibits membrane permeabilization by competing with Bax. PLoS Biol. 2008, 6:e147.
2. Bodet L., Gomez-Bougie P., Touzeau C., Dousset C., Descamps G., Maïga S., Avet-Loiseau H., Bataille R., Moreau P., Le Gouill S., et al. ABT-737 is highly effective against molecular subgroups of multiple myeloma. Blood 2011, 118:3901-3910.
3. Bouillet P., Metcalf D., Huang D.C.S., Tarlinton D.M., Kay T.W.H., Köntgen F., Adams J.M., Strasser A. Proapoptotic Bcl-2 relative Bim required for certain apoptotic responses, leukocyte homeostasis, and to preclude autoimmunity. Science 1999, 286:1735-1738.
4. Bruncko M., Oost T.K., Belli B.A., Ding H., Joseph M.K., Kunzer A., Martineau D., McClellan W.J., Mitten M., Ng S.C., et al. Studies leading to potent, dual inhibitors of Bcl-2 and Bcl-xL. J. Med. Chem. 2007, 50:641-662.
5. Buron N., Porceddu M., Brabant M., Desgué D., Racoeur C., Lassalle M., Péchoux C., Rustin P., Jacotot E., Borgne-Sanchez A. Use of human cancer cell lines mitochondria to explore the mechanisms of BH3 peptides and ABT-737-induced mitochondrial membrane permeabilization. PLoS ONE 2010, 5:e9924.
6. Certo M., Del Gaizo Moore V., Nishino M., Wei G., Korsmeyer S., Armstrong S.A., Letai A. Mitochondria primed by death signals determine cellular addiction to antiapoptotic BCL-2 family members. Cancer Cell 2006, 9:351-365.
7. Chauhan D., Velankar M., Brahmandam M., Hideshima T., Podar K., Richardson P., Schlossman R., Ghobrial I., Raje N., Munshi N., Anderson K.C. A novel Bcl-2/Bcl-X(L)/Bcl-w inhibitor ABT-737 as therapy in multiple myeloma. Oncogene 2007, 26:2374-2380.
8. Chen Y., Periasamy A. Characterization of two-photon excitation fluorescence lifetime imaging microscopy for protein localization. Microsc. Res. Tech. 2004, 63:72-80.
9. Chen Y., Mills J.D., Periasamy A. Protein localization in living cells and tissues using FRET and FLIM. Differentiation 2003, 71:528-541.
10. Chen L., Willis S.N., Wei A., Smith B.J., Fletcher J.I., Hinds M.G., Colman P.M., Day C.L., Adams J.M., Huang D.C. Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function. Mol. Cell 2005, 17:393-403.
11. Galluzzi L., Aaronson S.A., Abrams J., Alnemri E.S., Andrews D.W., Baehrecke E.H., Bazan N.G., Blagosklonny M.V., Blomgren K., Borner C., et al. Guidelines for the use and interpretation of assays for monitoring cell death in higher eukaryotes. Cell Death Differ. 2009, 16:1093-1107.
12. Giam M., Huang D.C., Bouillet P. BH3-proteins and their roles in programmed cell death. Oncogene 2008, 27:S128-S136.
13. Hann C.L., Daniel V.C., Sugar E.A., Dobromilskaya I., Murphy S.C., Cope L., Lin X., Hierman J.S., Wilburn D.L., Watkins D.N., Rudin C.M. Therapeutic efficacy of ABT-737, a selective inhibitor of BCL-2, in small cell lung cancer. Cancer Res. 2008, 68:2321-2328.
14. Hsu Y.T., Youle R.J. Bax in murine thymus is a soluble monomeric protein that displays differential detergent-induced conformations. J. Biol. Chem. 1998, 273:10777-10783.
15. Kirkin V., Joos S., Zörnig M. The role of Bcl-2 family members in tumorigenesis. Biochim. Biophys. Acta 2004, 1644:229-249.
16. Leber B., Lin J., Andrews D.W. Embedded together: the life and death consequences of interaction of the Bcl-2 family with membranes. Apoptosis 2007, 12:897-911.
17. Leber B., Lin J., Andrews D.W. Still embedded together binding to membranes regulates Bcl-2 protein interactions. Oncogene 2010, 29:5221-5230.
18. Lee E.F., Czabotar P.E., van Delft M.F., Michalak E.M., Boyle M.J., Willis S.N., Puthalakath H., Bouillet P., Colman P.M., Huang D.C., Fairlie W.D. A novel BH3 ligand that selectively targets Mcl-1 reveals that apoptosis can proceed without Mcl-1 degradation. J. Cell Biol. 2008, 180:341-355.
19. Lovell J.F., Billen L.P., Bindner S., Shamas-Din A., Fradin C., Leber B., Andrews D.W. Membrane binding by tBid initiates an ordered series of events culminating in membrane permeabilization by Bax. Cell 2008, 135:1074-1084.
20. Mason K.D., Vandenberg C.J., Scott C.L., Wei A.H., Cory S., Huang D.C., Roberts A.W. In vivo efficacy of the Bcl-2 antagonist ABT-737 against aggressive Myc-driven lymphomas. Proc. Natl. Acad. Sci. USA 2008, 105:17961-17966.
21. O'Connor L., Strasser A., O'Reilly L.A., Hausmann G., Adams J.M., Cory S., Huang D.C. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998, 17:384-395.
22. Oltersdorf T., Elmore S.W., Shoemaker A.R., Armstrong R.C., Augeri D.J., Belli B.A., Bruncko M., Deckwerth T.L., Dinges J., Hajduk P.J., et al. An inhibitor of Bcl-2 family proteins induces regression of solid tumours. Nature 2005, 435:677-681.
23. Peng J., Tan C., Roberts G.J., Nikolaeva O., Zhang Z., Lapolla S.M., Primorac S., Andrews D.W., Lin J. tBid elicits a conformational alteration in membrane-bound Bcl-2 such that it inhibits Bax pore formation. J. Biol. Chem. 2006, 281:35802-35811.
24. Periasamy A., Day R.N. Molecular Imaging, FRET Microscopy and Spectroscopy 2005, Oxford University Press, Oxford.
25. Puthalakath H., Strasser A. Keeping killers on a tight leash: transcriptional and post-translational control of the pro-apoptotic activity of BH3-proteins. Cell Death Differ. 2002, 9:505-512.
26. Renton J.P., Xu N., Clark J.J., Hansen M.R. Interaction of neurotrophin signaling with Bcl-2 localized to the mitochondria and endoplasmic reticulum on spiral ganglion neuron survival and neurite growth. J. Neurosci. Res. 2010, 88:2239-2251.
27. Schimmer A.D., Hedley D.W., Chow S., Pham N.A., Chakrabartty A., Bouchard D., Mak T.W., Trus M.R., Minden M.D. The BH3 domain of BAD fused to the Antennapedia peptide induces apoptosis via its alpha helical structure and independent of Bcl-2. Cell Death Differ. 2001, 8:725-733.
28. Shaner N.C., Campbell R.E., Steinbach P.A., Giepmans B.N., Palmer A.E., Tsien R.Y. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 2004, 22:1567-1572.
29. Vogler M., Weber K., Dinsdale D., Schmitz I., Schulze-Osthoff K., Dyer M.J., Cohen G.M. Different forms of cell death induced by putative BCL2 inhibitors. Cell Death Differ. 2009, 16:1030-1039.
30. Wang K., Yin X.M., Chao D.T., Milliman C.L., Korsmeyer S.J. BID: a novel BH3 domain-only death agonist. Genes Dev. 1996, 10:2859-2869.
31. Yang E., Zha J., Jockel J., Boise L.H., Thompson C.B., Korsmeyer S.J. Bad, a heterodimeric partner for Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 1995, 80:285-291.
32. Youle R.J., Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. Nat. Rev. Mol. Cell Biol. 2008, 9:47-59.
33. Zacharias D.A., Violin J.D., Newton A.C., Tsien R.Y. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 2002, 296:913-916.
34. Zhu W., Cowie A., Wasfy G.W., Penn L.Z., Leber B., Andrews D.W. Bcl-2 mutants with restricted subcellular location reveal spatially distinct pathways for apoptosis in different cell types. EMBO J. 1996, 15:4130-4141.