A homogeneous, high-throughput-compatible, fluorescence intensity-based assay for UDP-N-acetylenolpyruvylglucosamine reductase (MurB) with nanomolar product detection

Journal of Biomolecular Screening

Volumn 17, Issue 3, 2012, Pages 327-338

  Shapiro, Adam B ^a   Livchak, Stephania ^a   Gao, Ning ^a   Whiteaker, James ^a   Thresher, Jason ^a   Jahić, Haris ^a   Huang, Jian ^a   Gu, Rong Fang ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

assay; high throughput; MurB; MurC; N acetylenolpyruvylglucosamine reductase; peptidoglycan; polynucleotide phosphorylase

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALANINE; ANTIINFECTIVE AGENT; BACTERIAL ENZYME; FLUORESCENT DYE; LIGASE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OLIGOMER; OXIDOREDUCTASE; PEPTIDOGLYCAN; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; RNA; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE N ACETYLENOLPYRUVYLGLUCOSAMINE REDUCTASE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; URIDINE DIPHOSPHATE N ACETYLMURAMIC ACID; URIDINE DIPHOSPHATE N ACETYLMURAMYLALANINE LIGASE;

ARTICLE; BACTERIAL CELL WALL; BIOSYNTHESIS; CATALYSIS; CELL DEATH; CYTOPLASM; DRUG TARGETING; ENZYME INHIBITION; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; NONHUMAN; OLIGOMERIZATION; OXIDATION KINETICS; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; PROTEIN SYNTHESIS; PROTEIN TARGETING; REDUCTION KINETICS;

ADENOSINE DIPHOSPHATE; ANTI-BACTERIAL AGENTS; CARBOHYDRATE DEHYDROGENASES; CELL WALL; DRUG DISCOVERY; ESCHERICHIA COLI; FLUORESCENCE; HIGH-THROUGHPUT SCREENING ASSAYS; PEPTIDOGLYCAN; POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE;

BACTERIA (MICROORGANISMS);

EID: 84859056029     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111425188     Document Type: Article

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