Kinetic characterization of wild-type and S229A mutant MurB: Evidence for the role of Ser 229 as a general acid

Biochemistry

Volumn 36, Issue 4, 1997, Pages 796-805

  Benson, Timothy E ^a   Walsh, Christopher T ^a   Massey, Vincent ^b  

^a HARVARD MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; FLAVOPROTEIN; MUTANT PROTEIN; OXIDOREDUCTASE; PYRUVIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; SERINE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

ARTICLE; BINDING KINETICS; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; FLUORESCENCE; GENE MUTATION; ISOMERISM; NONHUMAN; OXIDATION REDUCTION POTENTIAL; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

BASE SEQUENCE; BINDING SITES; CARBOHYDRATE DEHYDROGENASES; CRYSTALLOGRAPHY, X-RAY; DNA PRIMERS; KINETICS; LIGANDS; MOLECULAR STRUCTURE; MUTAGENESIS, SITE-DIRECTED; NADP; OXIDATION-REDUCTION; POINT MUTATION; PROTONS; SERINE; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS);

EID: 0031047397     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi962220o     Document Type: Article

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