Intracellular Pb2+ content monitoring using a protein-based Pb2+ indicator

Toxicological Sciences

Volumn 126, Issue 2, 2012, Pages 436-445

  Chiu, Tai Yu ^a,b   Yang, De Ming ^a,b  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

Heavy metal ion biosensing; Live cell imaging; MerR

Indexed keywords

BINDING PROTEIN; LEAD; LEAD BINDING PROTEIN PBRR; METHIONINE; PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BIOSENSOR; CHEMICAL COMPOSITION; CONTROLLED STUDY; CUPRIAVIDUS; CUPRIAVIDUS METALLIDURANS; CYTOTOXICITY; ENVIRONMENTAL EXPOSURE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE CONSTRUCT; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRACELLULAR MEMBRANE; ION CONDUCTANCE; KIDNEY CELL; LONG TERM EXPOSURE; MOLECULAR DYNAMICS; SPECTRAL SENSITIVITY; AMINO ACID SEQUENCE; CELL LINE; CHEMISTRY; GENETIC PROCEDURES; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

CUPRIAVIDUS METALLIDURANS;

AMINO ACID SEQUENCE; BIOSENSING TECHNIQUES; CELL LINE; HUMANS; LEAD; MOLECULAR SEQUENCE DATA; PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 84859023835     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfs007     Document Type: Article

References (33)

1. Bannon, D. I., Portnoy, M. E., Olivi, L., Lees, P. S., Culotta, V. C., and Bressler, J. P. (2002). Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells. Biochem. Biophys. Res. Commun. 295, 978-984.
2. Barrineau, P., Gilbert, P., Jackson, W. J., Jones, C. S., Summers, A. O., and Wisdom, S. (1985). The structure of the mer operon. Basic Life Sci. 30, 707-718.
3. Borremans, B., Hobman, J. L., Provoost, A., Brown, N. L., and Van der Lelie, D. (2001). Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34. J. Bacteriol. 183, 5651-5658.
4. Brown, N. L., Stoyanov, J. V., Kidd, S. P., and Hobman, J. L. (2003). The MerR family of transcriptional regulators. FEMS Microbiol. Rev. 27, 145-163.
5. CDC Advisory Committee on Childhood Lead Poisoning Prevention (2007). Interpreting and managing blood lead levels < 10 microg/dL in children and reducing childhood exposures to lead: Recommendations of CDC's Advisory Committee on Childhood Lead Poisoning Prevention. MMWR Recomm Rep. 56, 1-16.
6. Centers for Disease Control and Prevention (CDC) (1991). Preventing Lead Poisoning in Young Children. U.S. Department of Health and Human Services, Atlanta, GA.
7. 2+ entry in living cells. Toxicol. Appl. Pharmacol. 227, 430-439.
8. Changela, A., Chen, K., Xue, Y., Holschen, J., Outten, C. E., O'Halloran, T. V., and Mondragón, A. (2003). Molecular basis of metalion selectivity and zeptomolar sensitivity by CueR. Science 301, 1383-1387.
9. Chen, C. T., and Huang, W. P. (2002). A highly selective fluorescent chemosensor for lead ions. J. Am. Chem. Soc. 124, 6246-6247.
10. Chen, P., Greenberg, B., Taghavi, S., Romano, C., van der Lelie, D., and He, C. (2005). An exceptionally selective lead(II)-regulatory protein from Ralstonia metallidurans: Development of a fluorescent lead(II) probe. Angew. Chem. Int. Ed. Engl. 44, 2715-2719.
11. Chiu, T. Y., Teng, H. C., Huang, P. C., Kao, F. J., and Yang, D. M. (2009). Dominant role of Orai1 with STIM1 on the cytosolic entry and cytotoxicity of lead ions. Toxicol. Sci. 110, 353-362.
12. Erdahl, W. L., Chapman, C. J., Taylor, R. W., and Pfeiffer, D. R. (2000). Ionomycin, a carboxylic acid ionophore, transports Pb(2+) with high selectivity. J. Biol. Chem. 275, 7071-7079.
13. Frantz, B., and O'Halloran, T. V. (1990). DNA distortion accompanies transcriptional activation by the metal-responsive gene-regulatory protein MerR. Biochemistry 29, 4747-4751.
14. Guo, H. B., Johs, A., Parks, J. M., Olliff, L., Miller, S. M., Summers, A. O., Liang, L., and Smith, J. C. (2010). Structure and conformational dynamics of the metalloregulatorMerR upon binding of Hg(II). J. Mol. Biol. 398, 555-568.
15. He, Q., Miller, E. W., Wong, A. P., and Chang, C. J. (2006). A selective fluorescent sensor for detecting lead in living cells. J. Am. Chem. Soc. 128, 9316-9317.
16. Kerper, L. E., and Hinkle, P. M. (1997). Cellular uptake of lead is activated by depletion of intracellular calcium stores. J. Biol. Chem. 272, 8346-8352.
17. Li, I. T., Pham, E., and Truong, K. (2006). Protein biosensors based on the principle of fluorescence resonance energy transfer for monitoring cellular dynamics. Biotechnol. Lett. 28, 1971-1982.
18. 2+ sensor. Angew. Chem. Int. Ed. Engl. 48, 3996-3998.
19. 2+ based on green fluorescent proteins and calmodulin. Nature 388, 882-887.
20. Mizuno, T., Murao, K., Tanabe, Y., Oda, M., and Tanaka, T. (2007). Metalion-dependent GFP emission in vivo by combining a circularly permutated green fluorescent protein with an engineered metal-ion-binding coiled-coil. J. Am. Chem. Soc. 129, 11378-11383.
21. Nagai, T., Yamada, S., Tominaga, T., Ichikawa, M., and Miyawaki, A. (2004). Expanded dynamic range of fluorescent indicators for Ca(2+) by circularly permuted yellow fluorescent proteins. Proc. Natl. Acad. Sci. U.S.A. 101, 10554-10559.
22. Outten, C. E., and O'Halloran, T. V. (2001). Femtomolar sensitivity of metalloregulatory proteins controlling zinc homeostasis. Science 292, 2488-2492.
23. Richmond, T. A., Takahashi, T. T., Shimkhada, R., and Bernsdorf, J. (2000). Engineered metal binding sites on green fluorescence protein. Biochem. Biophys. Res. Commun. 268, 462-465.
24. Rost, B., Yachdav, G., and Liu, J. (2004). The PredictProtein server. Nucleic Acids Res. 32, W321-W326.
25. Song, L., Caguiat, J., Li, Z., Shokes, J., Scott, R. A., Olliff, L., and Summers, A. O. (2004). Engineered single-chain, antiparallel, coiled coil mimics the MerR metal binding site. J. Bacteriol. 186, 1861-1868.
26. Song, L., Teng, Q., Phillips, R. S., Brewer, J. M., and Summers, A. O. (2007). 19F-NMR reveals metal and operator-induced allostery in MerR. J. Mol. Biol. 371, 79-92.
27. Taghavi, S., Lesaulnier, C., Monchy, S., Wattiez, R., Mergeay, M., and van der Lelie, D. (2009). Lead(II) resistance in Cupriavidus metallidurans CH34: Interplay between plasmid and chromosomally-located functions. Antonie Van Leeuwenhoek 96, 171-182.
28. Vinkenborg, J. L., Koay, M. S., and Merkx, M. (2010). Fluorescent imaging of transition metal homeostasis using genetically encoded sensors. Curr. Opin. Chem. Biol. 14, 231-237.
29. Walton, J. R. (1973). Granules containing lead in isolated mitochondria. Nature 243, 100-101.
30. Wegner, S. V., Arslan, H., Sunbul, M., Yin, J., and He, C. (2010). Dynamic copper(I) imaging in mammalian cells with a genetically encoded fluorescent copper(I) sensor. J. Am. Chem. Soc. 132, 2567-2569.
31. White, L. D., Cory-Slechta, D. A., Gilbert, M. E., Tiffany-Castiglioni, E., Zawia, N. H., Virgolini, M., Rossi-George, A., Lasley, S. M., Qian, Y. C., and Basha, M. R. (2007). New and evolving concepts in the neurotoxicology of lead. Toxicol. Appl. Pharmacol. 225, 1-27.
32. Williams, R. J. P., and Fraústo da Silva, J. J. R. (2000). The distribution of elements in cells. Coord. Chem. Rev. 200-202, 247-348.
33. Zeng, Q., Stålhandske, C., Anderson, M. C., Scott, R. A., and Summers, A. O. (1998). The core metal-recognition domain of MerR. Biochemistry 37, 15885-15895.