A comparative study on the expression, purification and functional characterization of human adiponectin in pichia pastoris and escherichia coli

International Journal of Molecular Sciences

Volumn 13, Issue 3, 2012, Pages 3549-3562

  Rothan, Hussin A ^a   Teh, Ser Huy ^a   Haron, Kamariah ^a   Mohamed, Zulqarnain ^a  

^a UNIVERSITY OF MALAYA   (Malaysia)

Author keywords

Adiponectin; Biological activity; E.coli; Pichia pastoris; Recombinant protein

Indexed keywords

ADIPONECTIN; BLOOD CLOTTING FACTOR 10A; HIGH DENSITY LIPOPROTEIN; LOW DENSITY LIPOPROTEIN; MALTOSE BINDING PROTEIN; RECOMBINANT PROTEIN; TRIACYLGLYCEROL; ADIPOQ PROTEIN, HUMAN; ISOPROTEIN;

ADIPOSE TISSUE; AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL STRAIN; COMPARATIVE STUDY; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE CONSTRUCT; GENE EXPRESSION SYSTEM; HUMAN; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROCESSING; PROTEIN PURIFICATION; TRIACYLGLYCEROL BLOOD LEVEL; WESTERN BLOTTING; BIOSYNTHESIS; CARDIOVASCULAR DISEASES; DIABETES MELLITUS, TYPE 2; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; PICHIA; PROCEDURES;

ESCHERICHIA COLI; EUKARYOTA; PICHIA PASTORIS;

ADIPONECTIN; CARDIOVASCULAR DISEASES; CLONING, MOLECULAR; DIABETES MELLITUS, TYPE 2; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HUMANS; PICHIA; PROTEIN ISOFORMS; RECOMBINANT PROTEINS;

EID: 84858994151     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms13033549     Document Type: Article

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