Separation and quantitative determination of 6α-hydroxycortisol and 6β-hydroxycortisol in human urine by high-performance liquid chromatography with ultraviolet absorption detection

Analytical and Bioanalytical Chemistry

Volumn 402, Issue 9, 2012, Pages 2945-2952

  Shibasaki, Hiromi ^a   Okamoto, Sawako ^a   Inoue, Risako ^a   Okita, Misato ^a   Yokokawa, Akitomo ^a   Furuta, Takashi ^a  

^a TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

Author keywords

6 hydroxycortisol; 6 Hydroxycortisol; Cortisol 6 hydroxylation clearance; HPLC UV

Indexed keywords

ETHYL ACETATES; GRADIENT ELUTION; HEALTHY SUBJECTS; HPLC METHOD; HPLC-UV; HUMAN URINE; HYBRID COLUMNS; INTER-ASSAY PRECISION; INTERNAL STANDARDS; LOWER LIMITS; LOWER LIMITS OF DETECTIONS; PHENOTYPING; QUANTITATIVE DETERMINATIONS; RELATIVE ERRORS; REPRODUCIBILITIES; REVERSED PHASE; SIMULTANEOUS DETERMINATIONS; TIME COURSE; ULTRAVIOLET ABSORPTION; URINARY METABOLITES; UV ABSORBANCE;

BODY FLUIDS; CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROXYLATION; SEPARATION; STEREOCHEMISTRY;

C (PROGRAMMING LANGUAGE);

6 BETA-HYDROXYCORTISOL; 6BETA HYDROXYHYDROCORTISONE; CYTOCHROME P450 3A; DRUG DERIVATIVE; HYDROCORTISONE;

ABSORPTION; ADULT; ARTICLE; CHEMISTRY; EVALUATION; FEMALE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; ULTRAVIOLET SPECTROPHOTOMETRY; URINE;

ABSORPTION; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTOCHROME P-450 CYP3A; FEMALE; HUMANS; HYDROCORTISONE; SPECTROPHOTOMETRY, ULTRAVIOLET; YOUNG ADULT;

EID: 84858702045     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-012-5714-3     Document Type: Article

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