Attenuation-based dual-fluorescent-protein reporter for screening translation inhibitors

Antimicrobial Agents and Chemotherapy

Volumn 56, Issue 4, 2012, Pages 1774-1783

  Osterman, Ilya A ^a   Prokhorova, Irina V ^a   Sysoev, Vasily O ^a   Boykova, Yulia V ^b   Efremenkova, Olga V ^b   Svetlov, Maxim S ^c   Kolb, Vyacheslav A ^c   Bogdanov, Alexey A ^a   Sergiev, Petr V ^a   Dontsova, Olga A ^a  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b GAUSE INSTITUTE OF NEW ANTIBIOTICS   (Russian Federation)

^c INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

AGAR; ANTIINFECTIVE AGENT; CERULEAN FLUORESCENT PROTEIN; FLUORESCENT DYE; FOSFOMYCIN; KANAMYCIN; NALIDIXIC ACID; NEOMYCIN; NORFLOXACIN; OFLOXACIN; POLYMYXIN; RED FLUORESCENT PROTEIN; RIFAMPICIN; SPECTINOMYCIN; STREPTOMYCIN; TETRACYCLINE; TOBRAMYCIN; TRYPTOPHAN; UNCLASSIFIED DRUG; VANCOMYCIN;

ARTICLE; ATTENUATION; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION; LIQUID CULTURE; NONHUMAN; OPERON; PRIORITY JOURNAL; RIBOSOME; SENSOR;

AGAR; AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; HIGH-THROUGHPUT SCREENING ASSAYS; LUMINESCENT PROTEINS; MICROMONOSPORA; MOLECULAR SEQUENCE DATA; OPERON; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PROTEIN SYNTHESIS INHIBITORS; RIBOSOMES; SIPHOVIRIDAE; TRANSCRIPTION, GENETIC; TRYPTOPHAN;

EID: 84858680411     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.05395-11     Document Type: Article

References (45)

1. Anko ML, Kurittu J, Karp M. 2002. An Escherichia coli biosensor strain for amplified and high throughput detection of antimicrobial agents. J. Biomol. Screen. 7:119-125. (Pubitemid 34493710)
2. Baba T, et al. 2006. Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2:2006.0008.
3. Bailey M, Chettiath T, Mankin AS. 2008. Induction of erm(C) expression by noninducing antibiotics. Antimicrob. Agents Chemother. 52: 866-874. (Pubitemid 351358364)
4. Beatty KE, Xie F, Wang Q, Tirrell DA. 2005. Selective dye-labeling of newly synthesized proteins in bacterial cells. J. Am. Chem. Soc. 127: 14150-14151. (Pubitemid 41457557)
5. Bianchi AA, Baneyx F. 1999. Stress responses as a tool to detect and characterize the mode of action of antibacterial agents. Appl. Environ. Microbiol. 65:5023-5027.
6. Brodersen DE, et al. 2000. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell 103:1143-1154. (Pubitemid 32037400)
7. Clarebout G, Leclercq R. 2002. Fluorescence assay for studying the ability of macrolides to induce production of ribosomal methylase. Antimicrob. Agents Chemother. 46:2269-2272. (Pubitemid 34665929)
8. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc. Natl. Acad. Sci. U. S. A. 97:6640-6645. (Pubitemid 30412767)
9. Davies J, Gilbert W, Gorini L. 1964. Streptomycin, suppression, and the code. Proc. Natl. Acad. Sci. U. S. A. 51:883-890.
10. Davies J, Jones DS, Khorana HG. 1966. A further study of misreading of codons induced by streptomycin and neomycin using ribopolynucleotides containing two nucleotides in alternating sequence as templates. J. Mol. Biol. 18:48-57.
11. Dunkle JA, Xiong L, Mankin AS, Cate JH. 2010. Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action. Proc. Natl. Acad. Sci. U. S. A. 107:17152-17157.
12. Fralick JA. 1996. Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J. Bacteriol. 178:5803-5805. (Pubitemid 26327687)
13. Galluzzi L, Karp M. 2003. Amplified detection of transcriptional and translational inhibitors in bioluminescent Escherichia coli K-12. J. Biomol. Screen. 8:340-346.
14. Gryczan TJ, Grandi G, Hahn J, Grandi R, Dubnau D. 1980. Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance. Nucleic Acids Res. 8:6081-6097. (Pubitemid 11100306)
15. Hartz D, McPheeters DS, Traut R, Gold L. 1988. Extension inhibition analysis of translation initiation complexes. Methods Enzymol. 164:419-425.
16. Horinouchi S, Weisblum B. 1980. Posttranscriptional modification of mRNA conformation: mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. U. S. A. 77:7079-7083. (Pubitemid 11190297)
17. Ippolito JA, et al. 2008. Crystal structure of the oxazolidinone antibiotic linezolid bound to the 50S ribosomal subunit. J. Med. Chem. 51:3353-3356. (Pubitemid 351874992)
18. Kostrzynska M, Leung KT, Lee H, Trevors JT. 2002. Green fluorescent protein-based biosensor for detecting SOS-inducing activity of genotoxic compounds. J. Microbiol. Methods 48:43-51. (Pubitemid 33145489)
19. Kurittu J, Karp M, Korpela M. 2000. Detection of tetracyclines with luminescent bacterial strains. Luminescence 15:291-297.
20. Lovett PS. 1996. Translation attenuation regulation of chloramphenicol resistance in bacteria-a review. Gene 179:157-162. (Pubitemid 26395089)
21. Lovett PS, Rogers EJ. 1996. Ribosome regulation by the nascent peptide. Microbiol. Rev. 60:366-385. (Pubitemid 26170911)
22. Meldal M, Tornoe CW. 2008. Cu-catalyzed azide-alkyne cycloaddition. Chem. Rev. 108:2952-3015.
23. Merino E, Jensen RA, Yanofsky C. 2008. Evolution of bacterial trp operons and their regulation. Curr. Opin. Microbiol. 11:78-86.
24. Merino E, Yanofsky C. 2005. Transcription attenuation: a highly conserved regulatory strategy used by bacteria. Trends Genet. 21:260-264. (Pubitemid 40558929)
25. Merzlyak EM, et al. 2007. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat. Methods 4:555-557. (Pubitemid 47214772)
26. Mitchell RJ, Gu MB. 2004. An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage. Appl. Microbiol. Biotechnol. 64:46-52. (Pubitemid 38392152)
27. Mohrle V, Stadler M, Eberz G. 2007. Biosensor-guided screening for macrolides. Anal. Bioanal. Chem. 388:1117-1125. (Pubitemid 47150032)
28. Nissen P, Hansen J, Ban N, Moore PB, Steitz TA. 2000. The structural basis of ribosome activity in peptide bond synthesis. Science 289:920-930. (Pubitemid 30659940)
29. Norman A, Hestbjerg Hansen L, Sorensen SJ. 2005. Construction of a ColD cda promoter-based SOS-green fluorescent protein whole-cell biosensor with higher sensitivity toward genotoxic compounds than constructs based on recA, umuDC, or sulA promoters. Appl. Environ. Microbiol. 71:2338-2346. (Pubitemid 40646975)
30. Pioletti M, et al. 2001. Crystal structures of complexes of the small ribosomal subunit with tetracycline, edeine and IF3. EMBO J. 20:1829-1839. (Pubitemid 32397386)
31. Qu X, et al. 2011. The ribosome uses two active mechanisms to unwind messenger RNA during translation. Nature 475:118-121.
32. Rizzo MA, Springer GH, Granada B, Piston DW. 2004. An improved cyan fluorescent protein variant useful for FRET. Nat. Biotechnol. 22:445-449. (Pubitemid 38451376)
33. Rommens J, MacKnight D, Pomeroy-Cloney L, Jay E. 1983. Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter. Nucleic Acids Res. 11:5921-5940.
34. Semenkov Yu P, Makarov EM, Makhno VI, Kirillov SV. 1982. Kinetic aspects of tetracycline action on the acceptor (A) site of Escherichia coli ribosomes. FEBS Lett. 144:125-129. (Pubitemid 13235627)
35. Shapiro E, Baneyx F. 2007. Stress-activated bioluminescent Escherichia coli sensors for antimicrobial agents detection. J. Biotechnol. 132:487-493. (Pubitemid 350051077)
36. Shapiro E, Baneyx F. 2002. Stress-based identification and classification of antibacterial agents: second-generation Escherichia coli reporter strains and optimization of detection. Antimicrob. Agents Chemother. 46:2490-2497. (Pubitemid 34793454)
37. Svetlov MS, Kommer A, Kolb VA, Spirin AS. 2006. Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family. Protein Sci. 15:242-247. (Pubitemid 43144996)
38. Takyar S, Hickerson RP, Noller HF. 2005. mRNA helicase activity of the ribosome. Cell 120:49-58. (Pubitemid 40094602)
39. Tenson T, Lovmar M, Ehrenberg M. 2003. The mechanism of action of macrolides, lincosamides and streptogramin B reveals the nascent peptide exit path in the ribosome. J. Mol. Biol. 330:1005-1014. (Pubitemid 36818898)
40. Tu D, Blaha G, Moore PB, Steitz TA. 2005. Structures of MLSBK antibiotics bound to mutated large ribosomal subunits provide a structural explanation for resistance. Cell 121:257-270. (Pubitemid 40546393)
41. Valtonen SJ, Kurittu JS, Karp MT. 2002. A luminescent Escherichia coli biosensor for the high throughput detection of beta-lactams. J. Biomol. Screen. 7:127-134. (Pubitemid 34493711)
42. VanBogelen RA, Neidhardt FC. 1990. Ribosomes as sensors of heat and cold shock in Escherichia coli. Proc. Natl. Acad. Sci. U. S. A. 87:5589-5593.
43. Vazquez-Laslop N, Thum C, Mankin AS. 2008. Molecular mechanism of drug-dependent ribosome stalling. Mol. Cell 30:190-202. (Pubitemid 351626687)
44. Weber K, Bartsch U, Stocking C, Fehse B. 2008. A multicolor panel of novel lentiviral "gene ontology" (LeGO) vectors for functional gene analysis. Mol. Ther. 16:698-706. (Pubitemid 351426171)
45. Zurawski G, Elseviers D, Stauffer GV, Yanofsky C. 1978. Translational control of transcription termination at the attenuator of the Escherichia coli tryptophan operon. Proc. Natl. Acad. Sci. U. S. A. 75:5988-5992. (Pubitemid 9093646)