Incidence and virulence determinants of verocytotoxin-producing Escherichia coli infections in the Brussels-Capital Region, Belgium, in 2008-2010

Journal of Clinical Microbiology

Volumn 50, Issue 4, 2012, Pages 1336-1345

  Buvens, Glenn ^a   De Gheldre, Yves ^b   Dediste, Anne ^c   De Moreau, Anne Isabelle ^d   Mascart, Georges ^e   Simon, Anne ^f   Allemeersch, Daniël ^g   Scheutz, Flemming ^h   Lauwers, Sabine ^a   Piérard, Denis ^a  

^a UNIVERSITY HOSPITAL BRUSSELS   (Belgium)

^b Centre Hospitalier interregional Edith Cavell   (Belgium)

^c SAINT PIERRE UNIVERSITY HOSPITAL   (Belgium)

^d IRIS   (Belgium)

^e HOP UNIV DES ENFANTS REINE FABIOLA   (Belgium)

^f CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

^g Cliniques de l'Europe Site St Elisabeth   (Belgium)

^h STATENS SERUM INSTITUT   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

AMIKACIN; AMOXICILLIN PLUS CLAVULANIC ACID; AMPICILLIN; AZTREONAM; CEFAZOLIN; CEFEPIME; CEFOTAXIME; CEFTAZIDIME; CEFTRIAXONE; CEFUROXIME; CHLORAMPHENICOL; CIPROFLOXACIN; GENTAMICIN; KANAMYCIN; MEROPENEM; NALIDIXIC ACID; PIPERACILLIN PLUS TAZOBACTAM; STREPTOMYCIN; SULFONAMIDE; TETRACYCLINE; TRIMETHOPRIM; VEROTOXIN;

ADULT; AGED; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL VIRULENCE; BELGIUM; BLOODY DIARRHEA; CHILD; CLUSTER ANALYSIS; CONTROLLED STUDY; EAE GENE; EPIDEMIC; ESCHERICHIA COLI; ESCHERICHIA COLI INFECTION; FECES ANALYSIS; GENE IDENTIFICATION; GENETIC VARIABILITY; HEMOLYTIC UREMIC SYNDROME; HOSPITAL LABORATORY; HUMAN; INCIDENCE; MAJOR CLINICAL STUDY; MULTIDRUG RESISTANCE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SEROTYPE; UNIVERSITY; VEROCYTOTOXIN 1 GENE; VEROCYTOTOXIN 2 GENE;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANTI-BACTERIAL AGENTS; BELGIUM; CHILD; CHILD, PRESCHOOL; DRUG RESISTANCE, BACTERIAL; ESCHERICHIA COLI INFECTIONS; FECES; FEMALE; HUMANS; INCIDENCE; INFANT; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PHYLOGEOGRAPHY; POLYMERASE CHAIN REACTION; SEASONS; SHIGA TOXINS; SHIGA-TOXIGENIC ESCHERICHIA COLI; URBAN POPULATION; VIRULENCE; VIRULENCE FACTORS; YOUNG ADULT;

ESCHERICHIA COLI;

EID: 84858609304     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.05317-11     Document Type: Article

References (63)

1. Andreoli SP, Trachtman H, Acheson DW, Siegler RL, Obrig TG. 2002. Hemolytic uremic syndrome: epidemiology, pathophysiology, and therapy. Pediatr. Nephrol. 17:293-298.
2. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K. 2004. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J. Clin. Microbiol. 42:1099-1108. (Pubitemid 38332832)
3. Bielaszewska M, Friedrich AW, Aldick T, Schurk-Bulgrin R, Karch H. 2006. Shiga toxin activatable by intestinal mucus in Escherichia coli isolated from humans: predictor for a severe clinical outcome. Clin. Infect. Dis. 43:1160-1167. (Pubitemid 44632799)
4. Bielaszewska M, et al. 22 June 2011. Characterisation of the Escherichia coli strain associated with an outbreak of haemolytic uraemic syndrome in Germany, 2011: a microbiological study. Lancet Infect. Dis. [Epub ahead of print.] doi:10.1016/S1473-3099(11)70165-7.
5. Blanco JE, et al. 2004. Serotypes, virulence genes, and intimin types of Shiga toxin (verotoxin)-producing Escherichia coli isolates from human patients: prevalence in Lugo, Spain, from 1992 through 1999. J. Clin. Microbiol. 42:311-319. (Pubitemid 38082738)
6. Brooks JT, et al. 2005. Non-O157 Shiga toxin-producing Escherichia coli infections in the United States, 1983-2002. J. Infect. Dis. 192:1422-1429. (Pubitemid 41401157)
7. Brunder W, Schmidt H, Frosch M, Karch H. 1999. The large plasmids of Shiga-toxin-producing Escherichia coli (STEC) are highly variable genetic elements. Microbiology 145(Pt 5):1005-1014. (Pubitemid 29231803)
8. Buvens G, Bogaerts P, Glupczynski Y, Lauwers S, Piérard D. 2010. Antimicrobial resistance testing of verocytotoxin-producing Escherichia coli and first description of TEM-52 extended-spectrum beta-lactamase in serogroup O26. Antimicrob. Agents Chemother. 54:4907-4909.
9. Buvens G, Lauwers S, Piérard D. 2010. Prevalence of subtilase cytotoxin in verocytotoxin-producing Escherichia coli isolated from humans and raw meats in Belgium. Eur. J. Clin. Microbiol. Infect. Dis. 29:1395-1399.
10. Buvens G, et al. 2011. Virulence profiling and quantification of verocytotoxin- producing Escherichia coli O145:H28 and O26:H11 isolated during an ice cream-related hemolytic uremic syndrome outbreak. Foodborne Pathog. Dis. 8:421-426.
11. Centers for Disease Control and Prevention. 2006. Importance of culture confirmation of Shiga toxin-producing Escherichia coli infection as illustrated by outbreaks of gastroenteritis-New York and North Carolina, 2005. MMWR Morb. Mortal. Wkly. Rep. 55:1042-1045.
12. Elbasha EH, Fitzsimmons TD, Meltzer MI. 2000. Costs and benefits of a subtype-specific surveillance system for identifying Escherichia coli O157:H7 outbreaks. Emerg. Infect. Dis. 6:293-297. (Pubitemid 30396386)
13. European Centre for Disease Prevention and Control. 2011. Shiga toxin/verotoxin-producing Escherichia coli in humans, food and animals in the EU/EEA, with special reference to the German outbreak strain STEC O104. ECDC, Stockholm, Sweden. http://ecdc.europa.eu/en/publications/Publications/1106-TER- EColi-joint-EFSA.pdf.
14. European Centre for Disease Prevention and Control. 2010. Surveillance report. Annual epidemiological report on communicable diseases in Europe, 2010. ECDC, Stockholm, Sweden. http://ecdc.europa.eu/en/publications/Publications/ 1011-SUR-Annual-Epidemiological-Report-on-Communicable-Diseases-in-Europe.pdf.
15. European Food Safety Authority. 5 July2011. Tracing seeds, in particular fenugreek (Trigonella foenum-graecum) seeds, in relation to the Shiga toxin- producing E. coli (STEC) O104:H4 2011 outbreaks in Germany and France. EFSA-Q-2011-00817. European Food Safety Authority, Parma, Italy. http://www.efsa.europa.eu/en/supporting/pub/176e.htm.
16. Farmer JJ III, Davis BR. 1985. H7 antiserum-sorbitol fermentation medium: a single tube screening medium for detecting Escherichia coli O157:H7 associated with hemorrhagic colitis. J. Clin. Microbiol. 22:620-625. (Pubitemid 15239286)
17. Frank C, et al. 2011. Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany-preliminary report. N. Engl. J. Med. 365:1771-1780.
18. Friedrich AW, et al. 2002. Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J. Infect. Dis. 185:74-84. (Pubitemid 34043019)
19. Reference deleted.
20. Hedican EB, et al. 2009. Characteristics of O157 versus non-O157 Shiga toxin-producing Escherichia coli infections in Minnesota, 2000-2006. Clin. Infect. Dis. 49:358-364.
21. Herold S, Paton JC, Paton AW. 2009. Sab, a novel autotransporter of locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli O113:H21, contributes to adherence and biofilm formation. Infect. Immun. 77:3234-3243.
22. Ishii Y, et al. 2005. Extended-spectrum beta-lactamase-producing Shiga toxin gene (Stx1)-positive Escherichia coli O26:H11: a new concern. J. Clin. Microbiol. 43:1072-1075. (Pubitemid 40364920)
23. Iyoda S, et al. 2000. Inducible stx2 phages are lysogenized in the enteroaggregative and other phenotypic Escherichia coli O86:HNM isolated from patients. FEMS Microbiol. Lett. 191:7-10.
24. Jenkins C, et al. 2003. Distribution of the saa gene in strains of Shiga toxin-producing Escherichia coli of human and bovine origins. J. Clin. Microbiol. 41:1775-1778. (Pubitemid 36428141)
25. Jenkins C, et al. 2003. Subtyping of virulence genes in verocytotoxin-producing Escherichia coli (VTEC) other than serogroup O157 associated with disease in the United Kingdom. J. Med. Microbiol. 52:941-947. (Pubitemid 38648132)
26. Johnson KE, Thorpe CM, Sears CL. 2006. The emerging clinical importance of non-O157 Shiga toxin-producing Escherichia coli. Clin. Infect. Dis. 43:1587-1595. (Pubitemid 44901903)
27. Kappeli U, Hachler H, Giezendanner N, Beutin L, Stephan R. 2011. Human infections with non-O157 Shiga toxin-producing Escherichia coli, Switzerland, 2000-2009. Emerg. Infect. Dis. 17:180-185.
28. Kappeli U, Hachler H, Giezendanner N, Cheasty T, Stephan R. 2011. Shiga toxin-producing Escherichia coli O157 associated with human infections in Switzerland, 2000-2009. Epidemiol. Infect. 139:1097-1104.
29. Karch H, Meyer T. 1989. Single primer pair for amplifying segments of distinct Shiga-like-toxin genes by polymerase chain reaction. J. Clin. Microbiol. 27:2751-2757. (Pubitemid 20001341)
30. Karch H, Tarr PI, Bielaszewska M. 2005. Enterohaemorrhagic Escherichia coli in human medicine. Int. J. Med. Microbiol. 295:405-418. (Pubitemid 41225453)
31. Karmali MA, et al. 2003. Association of genomic O island 122 of Escherichia coli EDL 933 with verocytotoxin-producing Escherichia coli seropathotypes that are linked to epidemic and/or serious disease. J. Clin. Microbiol. 41:4930-4940. (Pubitemid 37429898)
32. Kobayashi H, et al. 2001. Prevalence and characteristics of Shiga toxin-producing Escherichia coli from healthy cattle in Japan. Appl. Environ. Microbiol. 67:484-489. (Pubitemid 32045666)
33. Kon M, et al. 2005. Cefotaxime-resistant Shiga toxin-producing Escherichia coli O26:H11 isolated from a patient with diarrhea. Kansenshogaku Zasshi 79:161-168.
34. Machado J, Grimont F, Grimont PA. 2000. Identification of Escherichia coli flagellar types by restriction of the amplified fliC gene. Res. Microbiol. 151:535-546.
35. Marcon MJ, Kiska DL, Riddell SW, Gilligan P. 2011. Should all stools be screened for Shiga toxin-producing Escherichia coli? J. Clin. Microbiol. 49:2390-2397.
36. McPherson M, Kirk MD, Raupach J, Combs B, Butler JR. 2011. Economic costs of Shiga toxin-producing Escherichia coli infection in Australia. Foodborne Pathog. Dis. 8:55-62.
37. Melton-Celsa AR, O'Brien AD. 1998. Structure, biology, and relative toxicity of Shiga toxin family members for cells and animals, p 121-128. In Kaper JB, O'Brien AD (ed), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, DC.
38. Mora A, et al. 2005. Antimicrobial resistance of Shiga toxin (verotoxin)- producing Escherichia coli O157:H7 and non-O157 strains isolated from humans, cattle, sheep and food in Spain. Res. Microbiol. 156:793-806. (Pubitemid 41242797)
39. Morabito S, et al. 2001. Detection and characterization of Shiga toxin-producing Escherichia coli in feral pigeons. Vet. Microbiol. 82:275-283. (Pubitemid 32695376)
40. Morabito S, et al. 1998. Enteroaggregative, Shiga toxin-producing Escherichia coli O111:H2 associated with an outbreak of hemolytic-uremic syndrome. J. Clin. Microbiol. 36:840-842. (Pubitemid 28093282)
41. Mossoro C, et al. 2002. Chronic diarrhea, hemorrhagic colitis, and hemolytic- uremic syndrome associated with HEp-2 adherent Escherichia coli in adults infected with human immunodeficiency virus in Bangui, Central African Republic. J. Clin. Microbiol. 40:3086-3088. (Pubitemid 35284997)
42. Nataro JP, Bopp CA, Fields PI, Kaper JB, Strockbine NA. 2011. Escherichia, Shigella, and Salmonella, p 603-626. In Versalovic J, et al (ed), Manual of clinical microbiology, 10th ed. ASM Press, Washington, DC.
43. Nataro JP, Kaper JB. 1998. Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11:142-201. (Pubitemid 28046019)
44. Orth D, et al. 2007. The Shiga toxin genotype rather than the amount of Shiga toxin or the cytotoxicity of Shiga toxin in vitro correlates with the appearance of the hemolytic uremic syndrome. Diagn. Microbiol. Infect. Dis. 59:235-242. (Pubitemid 350016692)
45. Paton AW, et al. 2006. AB5 subtilase cytotoxin inactivates the endoplasmic reticulum chaperone BiP. Nature 443:548-552. (Pubitemid 44527286)
46. PatonAW,Paton JC. 1998. Detection and characterization of Shiga toxigenic Escherichia coli by using multiplex PCR assays for stx1, stx2, eaeA, enterohemorrhagic E. coli hlyA, rfbO111, and rfbO157. J. Clin. Microbiol. 36:598-602. (Pubitemid 28060342)
47. Paton AW, Paton JC. 2002. Direct detection and characterization of Shiga toxigenic Escherichia coli by multiplex PCR for stx1, stx2, eae, ehxA, and saa. J. Clin. Microbiol. 40:271-274. (Pubitemid 34027638)
48. Paton AW, Srimanote P, Talbot UM, Wang H, Paton JC. 2004. A new family of potent AB(5) cytotoxins produced by Shiga toxigenic Escherichia coli. J. Exp. Med. 200:35-46. (Pubitemid 38924726)
49. Paton AW, Srimanote P, Woodrow MC, Paton JC. 2001. Characterization of Saa, a novel autoagglutinating adhesin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains that are virulent for humans. Infect. Immun. 69:6999-7009. (Pubitemid 32995460)
50. Piérard D, et al. 1999. A case-control study of sporadic infection with O157 and non-O157 verocytotoxin-producing Escherichia coli. Epidemiol. Infect. 122:359-365. (Pubitemid 29376071)
51. Piérard D, Stevens D, Moriau L, Lior H, Lauwers S. 1997. Isolation and virulence factors of verocytotoxin-producing Escherichia coli in human stool samples. Clin. Microbiol. Infect. 3:531-540. (Pubitemid 27483234)
52. Pradel N, et al. 2000. Prevalence and characterization of Shiga toxin-producing Escherichia coli isolated from cattle, food, and children during a one-year prospective study in France. J. Clin. Microbiol. 38:1023-1031. (Pubitemid 30140553)
53. Prager R, Fruth A, Siewert U, Strutz U, Tschape H. 2009. Escherichia coli encoding Shiga toxin 2f as an emerging human pathogen. Int. J. Med. Microbiol. 299:343-353.
54. Rivero MA, Passucci JA, Rodriguez EM, Parma AE. 2010. Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina. J. Med. Microbiol. 59:345-352.
55. Roest HI, et al. 2007. Antibiotic resistance in Escherichia coli O157 isolated between 1998 and 2003 in The Netherlands. Tijdschr. Diergeneeskd. 132:954-958. (In Dutch.) (Pubitemid 350304442)
56. Scallan E, et al. 2011. Foodborne illness acquired in the United States-major pathogens. Emerg. Infect. Dis. 17:7-15.
57. Scavia G, et al. 2011. Similarity of Shiga toxin-producing Escherichia coli O104:H4 strains from Italy and Germany. Emerg. Infect. Dis. 17:1957-1958.
58. Scheutz F, et al. 2011. Characteristics of the enteroaggregative Shiga toxin/verotoxin-producing Escherichia coli O104:H4 strain causing the outbreak of haemolytic uraemic syndrome in Germany, May to June 2011. Euro Surveill. 16(24):pii=19889.
59. - strains that do not produce Shiga toxin: phenotypic and genetic characterization of isolates associated with diarrhea and hemolytic-uremic syndrome. J. Clin. Microbiol. 37:3491-3496.
60. Schmidt H, et al. 2000. A new Shiga toxin 2 variant (Stx2f) from Escherichia coli isolated from pigeons. Appl. Environ. Microbiol. 66:1205-1208. (Pubitemid 30130048)
61. van Duynhoven YT, et al. 2008. Prevalence, characterisation and clinical profiles of Shiga toxin-producing Escherichia coli in The Netherlands. Clin. Microbiol. Infect. 14:437-445. (Pubitemid 351513931)
62. Wickham ME, et al. 2006. Bacterial genetic determinants of non-O157 STEC outbreaks and hemolytic-uremic syndrome after infection. J. Infect. Dis. 194:819-827. (Pubitemid 44344131)
63. York MK, Rodrigues-Wong P, Church DL. 2010. Fecal culture for aerobic pathogens of gastroenteritis, p. 3.8.1.1-3.8.5.1. In Garcia LS (ed), Clinical microbiology procedures handbook, 3rd ed. ASM Press, Washington, DC.