Cellular fura-2 Manganese Extraction Assay (CFMEA)

Current Protocols in Toxicology

Volumn , Issue SUPPL.48, 2011, Pages

  Kwakye, Gunnar F ^a   Li, Daphne ^a   Kabobel, Olympia A ^a   Bowman, Aaron B ^a  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Fura 2; High throughput assay; Manganese; Metal transport

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; CHLORINE; DOUBLE STRANDED DNA; EDETIC ACID; FURA 2; MANGANESE; MANGANESE CHLORIDE; NUCLEIC ACID; REAGENT; SODIUM CHLORIDE;

ANALYTIC METHOD; ANIMAL CELL CULTURE; ARTICLE; ASTROCYTE; CELL ASSAY; CELL COUNT; CELL DENSITY; CELL IMMORTALIZATION; CELL LEVEL; CELL TYPE; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); CORPUS STRIATUM; INTRACELLULAR TRANSPORT; METAL EXTRACTION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DETERMINATION; SALMON; TESTIS;

MAMMALIA; MURINAE;

EID: 84858284715     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx1218s48     Document Type: Article

References (28)

1. Ahn, S.J., Costa, J., and Emanuel, J.R. 1996. PicoGreen quantitation of DNA: Effective evaluation of samples pre- or post-PCR. Nucleic Acids Res. 24:2623-2625.
2. Aschner, M., Gannon, M., and Kimelberg, H.K. 1992. Manganese uptake and efflux in cultured rat astrocytes. J. Neurochem. 58:730-735.
3. Aschner, M., Guilarte, T.R., Schneider, J.S., and Zheng, W. 2007. Manganese: Recent advances in understanding its transport and neurotoxicity. Toxicol. Appl. Pharmacol. 221:131-147.
4. Baruthio, F., Guillard, O., Arnaud, J., Pierre, F., and Zawislak, R. 1988. Determination ofmanganese in biological materials by electrothermal atomic absorption spectrometry: A review. Clin. Chem. 34:227-234.
5. Butterworth, J. 1986. Changes in nine enzyme markers for neurons, glia, and endothelial cells in agonal state and Huntington's disease caudate nucleus. J. Neurochem. 47:583-587.
6. Cobbold, P.H. and Rink, T.J. 1987. Fluorescence and bioluminescence measurement of cytoplasmic free calcium. Biochem. J. 248:313-328.
7. Enger, Ø. 1996. Use of the fluorescent dye PicoGreen for quantification of PCR products after agarose gel electrophoresis. Biotechniques 21:372-374.
8. Erikson, K.M. and Aschner, M. 2003. Manganese neurotoxicity and glutamate-GABA interaction. Neurochem. Int. 43:475-480.
9. Erikson, K.M., Syversen, T., Aschner, J., and Aschner, M. 2005. Interactions between excessive manganese-exposure and dietary irondeficiency in neurodegeneration. Environ. Toxicol. Pharmacol. 19:415-421.
10. Forbes, J.R. and Gros, P. 2003. Iron, manganese, and cobalt transport by Nramp1 (Slc11a1) and Nramp2 (Slc11a2) expressed at the plasma membrane. Blood 102:1884-1892.
11. 2+ indicatorswith greatly improved fluorescence properties. J. Biol.Chem. 260:3440-3450.
12. Hurley, L.S. and Keen, C.L. 1987. Manganese In Trace Elements in Human Health and Animal Nutrition (E. Underwood and W. Mertz, eds.) pp. 185-225. Academic Press, New York.
13. Kwakye, G.F., Li, D., and Bowman, A.B. 2011. Novel high-throughput assay to assess cellular manganese levels in a striatal cell line model of Huntington's disease confirms a deficit in manganese accumulation. Neurotoxicology Jan. 14 [Epub ahead of print].
14. Lim, D., Feedrizzi, L., Tartari, M., Zuccato, C., Cattaneo, E., Brini, M., and Carafoli, E. 2007. Calcium homeostasis and mitochondrial dysfunction in striatal neurons of Huntington disease. J. Biol. Chem. 283:5780-5789.
15. Merritt, J.E., Jacob, R., and Hallam, T.J. 1989. Use of manganese to discriminate between calcium influx and mobilization from internal stores in stimulated human neutrophils. J. Biol. Chem. 264:1522-1527.
16. Molecular Probes. 1996. PicoGreen dsDNA Quantitation Reagent and Kit Instructions. Eugene, Oregon.
17. 2+ handling in Huntington's disease striatal cells: Effect of histone deacetylase inhibitors. J. Neurosci. 26:11174-11186.
18. Picard, V., Govoni, G., Jabado, N., and Gros, P. 2000. Nramp 2 (DCT1/DMT1) expressed at the plasma membrane transports iron and other divalent cations into a calcein-accessible cytoplasmic pool. J. Biol. Chem. 275:35738-35745.
19. Seville, M., West, A.B., and McHenry, C.S. 1996. Fluorometric assay for DNA polymerases and reverse transcriptase. Biotechniques 21:664-672.
20. 2+ signals. Biophys. J. 71:1048-1056.
21. Tang, T.S., Tu, H., Maximov, A., Wang, Z., Wellington, C.L., Hayden, M.R., and Bezprozvanny, I. 2003. Huntingtin and huntingtin-associated protein 1 influence neuronal calcium signaling mediated by inositol-(1,4,5) triphosphate receptor type 1. Neuron 39:227-239.
22. Trettel, F., Rigamonti, D., Hilditch-Maguire, P., Wheeler, V.C., Sharp, A.H., Persichetti, F., Cattaneo, E., andMacDonald,M.E. 2000. Dominant phenotypes produced by the HD mutation in STHdh(Q111) striatal cells. Hum. Mol. Genet. 9:2799-2809.
23. Tsien, R.Y. 1989. Fluorescent probes of cell signaling. Ann. Rev. Neurosci. 12:227-253.
24. 2+ with quin-2. Methods Enzymol. 172:230-262.
25. 2+ channels. Trends Pharmacol. Sci. 12:349-354.
26. Williams, B.B., Kwakye, G.F., Wegrzynowicz, M., Li, D., Aschner, M., Erikson, K.M., and Bowman, A.B. 2010a. Altered manganese homeostasis and manganese toxicity in a Huntington's disease striatal cell model are not explained by defects in the iron transport system. Toxicol. Sci. 117:169-179.
27. Williams, B.B., Li, D., Wegrzynowicz, M., Vadodaria, B.K., Anderson, J.G., Kwakye, G.F., Aschner, M., Erikson, K.M., and Bowman, A.B. 2010b. Disease-toxicant screen reveals a neuroprotective interaction between Huntington's disease and manganese exposure. J. Neurochem. 112:227-237.
28. 2+ homeostasis and expression of NMDA receptor subunits in primary cultured neurons. Neurotoxicology 30:941-949.