GFP immunogold staining, from light to electron microscopy, in mammalian cells

Micron

Volumn 43, Issue 5, 2012, Pages 589-599

  Sirerol Piquer, M Salomé ^a   Cebrián Silla, Arantxa ^b   Alfaro Cervelló, Clara ^c   Gomez Pinedo, Ulises ^d   Soriano Navarro, Mario ^a   Verdugo, José Manuel García ^a,b  

^a CENTRO DE INVESTIGACIÓN PRÍNCIPE FELIPE   (Spain)

^b UNIVERSITY OF VALENCIA   (Spain)

^c UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^d HOSPITAL CLÍNICO SAN CARLOS   (Spain)

Author keywords

Colloidal gold; Electron microscopy; Embedding; GFP; Immunogold; Silver enhancement

Indexed keywords

COLLOIDAL GOLD; EMBEDDING; GFP; IMMUNOGOLD; SILVER ENHANCEMENT;

COLLOIDS; ELECTRON MICROSCOPES; ELECTRON MICROSCOPY; EPOXY RESINS; GENE EXPRESSION; TISSUE; TISSUE CULTURE;

SILVER;

FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN;

ANIMAL; HUMAN; IMMUNOHISTOCHEMISTRY; METHODOLOGY; REVIEW; STAINING;

ANIMALS; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; IMMUNOHISTOCHEMISTRY; STAINING AND LABELING;

MAMMALIA;

EID: 84858154218     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micron.2011.10.008     Document Type: Review

References (54)

1. Alfaro-Cervello C., Soriano-Navarro M., Gomez-PInedo U., Garcia-Verdugo J.M. Correlation of light and electron microscopy for immunogold staining. GFP immunogold, tool in biological research. Microscopy: Science, Technology, Applications and Education 2010, pp. 844-850.
2. Baraban S.C., Southwell D.G., Estrada R.C., Jones D.L., Sebe J.Y., Alfaro-Cervello C., Garcia-Verdugo J.M., Rubenstein J.L., Alvarez-Buylla A. Reduction of seizures by transplantation of cortical GABAergic interneuron precursors into Kv1.1 mutant mice. Proc. Natl. Acad. Sci. U.S.A. 2009, 106(36):15472-15477.
3. Bartlett P.A., Bauer B.J.S.S. Synthesis of water-soluble undecagold cluster compounds of potential importance in electron microscopic and other studies of biological systems. J. Am. Chem. Soc. 1978, 100(16):5085-5089.
4. Bastiaens P.I., Pepperkok R. Observing proteins in their natural habitat: the living cell. Trends Biochem. Sci. 2000, 25(12):631-637.
5. Bates P., Young J.A., Varmus H.E. A receptor for subgroup A Rous sarcoma virus is related to the low density lipoprotein receptor. Cell 1993, 74(6):1043-1051.
6. Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 1994, 263(5148):802-805.
7. Cormack B.P., Valdivia R.H., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173(1(Spec No)):33-38.
8. Danscher G. Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy. Histochemistry 1981, 71(1):1-16.
9. Danscher G., Hacker G., Hauser-Kronberger C., Grimelius L. Trends in autometallographic silver amplification of colloidal gold particles. Immunogold Silver Staining: Principles, Methods and Applications 1995, 8. M. Hayat (Ed.).
10. Doetsch F., Caille I., Lim D.A., Garcia-Verdugo J.M., Alvarez-Buylla A. Subventricular zone astrocytes are neural stem cells in the adult mammalian brain. Cell 1999, 97(6):703-716.
11. El Maarouf A., Petridis A.K., Rutishauser U. Use of polysialic acid in repair of the central nervous system. Proc. Natl. Acad. Sci. U.S.A. 2006, 103(45):16989-16994.
12. Faulk W.P., Taylor G.M. An immunocolloid method for the electron microscope. Immunochemistry 1971, 8(11):1081-1083.
13. Follet-Gueye M.L., Pagny S., Faye L., Gomord V., Driouich A. An improved chemical fixation method suitable for immunogold localization of green fluorescent protein in the Golgi apparatus of tobacco bright yellow (BY-2) cells. J. Histochem. Cytochem. 2003, 51(7):931-940.
14. Frosst P., Guan T., Subauste C., Hahn K., Gerace L. Tpr is localized within the nuclear basket of the pore complex and has a role in nuclear protein export. J. Cell Biol. 2002, 156(4):617-630.
15. Gandia C., Arminan A., Garcia-Verdugo J.M., Lledo E., Ruiz A., Minana M.D., Sanchez-Torrijos J., Paya R., Mirabet V., Carbonell-Uberos F., et al. Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction. Stem Cells 2008, 26(3):638-645.
16. Hainfeld J.F., Furuya F.R. A 1.4-nm gold cluster covalently attached to antibodies improves immunolabeling. J. Histochem. Cytochem. 1992, 40(2):177-184.
17. Hainfeld J.F., Robinson J.M. New frontiers in gold labeling: symposium overview. J. Histochem. Cytochem. 2000, 48(4):459-460.
18. Hayat M. Fixation Principles and techniques of electron microscopy. Biol. Appl. 1981, 9:4.
19. Heim R., Cubitt A.B., Tsien R.Y. Improved green fluorescence. Nature 1995, 373(6516):663-664.
20. Heim R., Prasher D.C., Tsien R.Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 1994, 91(26):12501-12504.
21. Holgate C.S., Jackson P., Cowen P.N., Bird C.C. Immunogold-silver staining: new method of immunostaining with enhanced sensitivity. J. Histochem. Cytochem. 1983, 31(7):938-944.
22. Holland E.C., Varmus H.E. Basic fibroblast growth factor induces cell migration and proliferation after glia-specific gene transfer in mice. Proc. Natl. Acad. Sci. U.S.A. 1998, 95(3):1218-1223.
23. Humbel B.M., Sibon O.C., Stierhof Y.D., Schwarz H. Ultra-small gold particles and silver enhancement as a detection system in immunolabeling and in situ hybridization experiments. J. Histochem. Cytochem. 1995, 43(7):735-737.
24. Inouye S., Tsuji F.I. Aequorea green fluorescent protein expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 1994, 341(2-3):277-280.
25. Kremerskothen J., Plaas C., Kindler S., Frotscher M., Barnekow A. Synaptopodin, a molecule involved in the formation of the dendritic spine apparatus, is a dual actin/alpha-actinin binding protein. J. Neurochem. 2005, 92(3):597-606.
26. Leitinger G., Pabst M.A., Kral K. Gold toning preserves integrity of silver enhanced immunogold particles during osmium tetroxide treatment for demonstration of a biogenic amine. Brain Res. Brain Res. Protoc. 2000, 5(1):30-38.
27. Lippincott-Schwartz J., Patterson G.H. Development and use of fluorescent protein markers in living cells. Science 2003, 300(5616):87-91.
28. Lippincott-Schwartz J., Snapp E., Kenworthy A. Studying protein dynamics in living cells. Nat. Rev. Mol. Cell Biol. 2001, 2(6):444-456.
29. Mazo M., Gavira J.J., Abizanda G., Moreno C., Ecay M., Soriano M., Aranda P., Collantes M., Alegria E., Merino J., et al. Transplantation of mesenchymal stem cells exerts a greater long-term effect than bone marrow mononuclear cells in a chronic myocardial infarction model in rat. Cell Transplant 2010, 19(3):313-328. (Epub 2009).
30. Nakane P.K., Pierce G.B. Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigens. J. Cell Biol. 1967, 33(2):307-318.
31. Ormo M., Cubitt A.B., Kallio K., Gross L.A., Tsien R.Y., Remington S.J. Crystal structure of the Aequorea victoria green fluorescent protein. Science 1996, 273(5280):1392-1395.
32. Orsulic S. An RCAS-TVA-based approach to designer mouse models. Mamm. Genome 2002, 13(10):543-547.
33. Oyasu M., Fujimiya M., Kashiwagi K., Ohmori S., Imaeda H., Saito N. Immunogold electron microscopic demonstration of distinct submembranous localization of the activated gammaPKC depending on the stimulation. J. Histochem. Cytochem. 2008, 56(3):253-265.
34. Pluchino S., Zanotti L., Brambilla E., Rovere-Querini P., Capobianco A., Alfaro-Cervello C., Salani G., Cossetti C., Borsellino G., Battistini L. others. Immune regulatory neural stem/precursor cells protect from central nervous system autoimmunity by restraining dendritic cell function. PLoS One 2009, 4(6):e5959.
35. Pohl K., Stierhof Y.D. Action of gold chloride (gold toning) on silver-enhanced 1nm gold markers. Microsc. Res. Tech. 1998, 42(1):59-65.
36. Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene 1992, 111(2):229-233.
37. Reynolds E.S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 1963, 17:208-212.
38. Riemann D., Hansen G.H., Niels-Christiansen L., Thorsen E., Immerdal L., Santos A.N., Kehlen A., Langner J., Danielsen E.M. Caveolae/lipid rafts in fibroblast-like synoviocytes: ectopeptidase-rich membrane microdomains. Biochem. J. 2001, 354(Pt 1):47-55.
39. Robinson J.M., Takizawa T., Vandre D.D. Enhanced labeling efficiency using ultrasmall immunogold probes: immunocytochemistry. J. Histochem. Cytochem. 2000, 48(4):487-492.
40. Roux S., Colasante C., Saint Cloment C., Barbier J., Curie T., Girard E., Molgo J., Brulet P. Internalization of a GFP-tetanus toxin C-terminal fragment fusion protein at mature mouse neuromuscular junctions. Mol. Cell. Neurosci. 2005, 30(1):79-89.
41. Sawada H., Esaki M. Use of nanogold followed by silver enhancement and gold toning for preembedding immunolocalization in osmium-fixed, Epon-embedded tissues. J. Electron Microsc. (Tokyo) 1994, 43(6):361-366.
42. Sawada H., Esaki M. A practical technique to postfix nanogold-immunolabeled specimens with osmium and to embed them in Epon for electron microscopy. J. Histochem. Cytochem. 2000, 48(4):493-498.
43. Schikorski T. Pre-embedding immunogold localization of antigens in mammalian brain slices. Immunoelectron Microscopy: Methods and Protocols 2010, 133-144. S. Schawartzbach, T. Osafune (Eds.).
44. Shima D.T., Haldar K., Pepperkok R., Watson R., Warren G. Partitioning of the Golgi apparatus during mitosis in living HeLa cells. J. Cell Biol. 1997, 137(6):1211-1228.
45. Shimomura O., Johnson F.H., Saiga Y. Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J. Cell. Comp. Physiol. 1962, 59:223-239.
46. Singer S.J. Preparation of an electron-dense antibody conjugate. Nature 1959, 183(4674):1523-1524.
47. Smigova J., Juda P., Cmarko D., Raska I. Fine structure of the "PcG body" in human U-2 OS cells established by correlative light-electron microscopy. Nucleus 2011, 2(3):219-228.
48. Tavare J.M., Fletcher L.M., Welsh G.I. Using green fluorescent protein to study intracellular signalling. J. Endocrinol. 2001, 170(2):297-306.
49. Tsien R.Y. The green fluorescent protein. Annu. Rev. Biochem. 1998, 67:509-544.
50. Viens A., Harper F., Pichard E., Comisso M., Pierron G., Ogryzko V. Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform. J. Histochem. Cytochem. 2008, 56(10):911-919.
51. Ward W.W., Cody C.W., Hart R.C., Cormier M.J. Spectrophotometric identity of the energy transfer chromophores in Renilla an Aequorea green-fluorescent proteins. Photochem. Photobiol. 1980, 31(6):611-615.
52. Yamashita T., Ninomiya M., Hernandez Acosta P., Garcia-Verdugo J.M., Sunabori T., Sakaguchi M., Adachi K., Kojima T., Hirota Y., Kawase T., et al. Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum. J. Neurosci. 2006, 26(24):6627-6636.
53. Young J.A., Bates P., Varmus H.E. Isolation of a chicken gene that confers susceptibility to infection by subgroup A avian leukosis and sarcoma viruses. J. Virol. 1993, 67(4):1811-1816.
54. Zhang B.J., Yamashita M., Fields R., Kusano K., Gainer H. EGFP-tagged vasopressin precursor protein sorting into large dense core vesicles and secretion from PC12 cells. Cell. Mol. Neurobiol. 2005, 25(3-4):581-605.