Use of protein biotinylation in vivo for immunoelectron microscopic localization of a specific protein isoform

Journal of Histochemistry and Cytochemistry

Volumn 56, Issue 10, 2008, Pages 911-919

  Viens, Antoine ^a   Harper, Francis ^b   Pichard, Evelyne ^b   Comisso, Martine ^a   Pierron, Gérard ^b   Ogryzko, Vasily ^a,c  

^a INSTITUT GUSTAVE ROUSSY   (France)

^b INSTITUT ANDRÉ LWOFF   (France)

^c IGR   (France)

Author keywords

Biotin; Electron microscopy; PML; Postembedding; SUMO2

Indexed keywords

BIOTIN; ISOPROTEIN; LIGASE; NUCLEAR PROTEIN; PROTEIN BIRA; UNCLASSIFIED DRUG;

ARTICLE; BIOTINYLATION; CLONE; CONTROLLED STUDY; COVALENT BOND; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMMUNOELECTRON MICROSCOPY; IMMUNOGOLD LABELING; IN VIVO STUDY; MORPHOLOGY; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION;

MAMMALIA;

EID: 52949105379     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2008.951624     Document Type: Article

References (20)

1. Arhel NJ, Souquere-Besse S, Charneau P (2006) Wild-type and central DNA flap defective HIV-1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels. Retrovirology 26:38-46
2. Beckett D, Kovaleva E, Schatz PJ (1999) A minimal peptide substrate in biotin holoenzyme synthetase-catalyzed biotinylation. Protein Sci 8:921-929
3. Condemine W, Takahashi Y, Zhu J, Puvion-Dutilleul F, Guegan S, Janin A, de The H (2006) Characterization of endogenous human promyelocytic leukemia isoforms. Cancer Res 66:6192-6198
4. Cull MG, Schatz PJ (2000) Biotinylation of proteins in vivo and in vitro using small peptide tags. Methods Enzymol 326:430-440
5. de Boer E, Rodriguez P, Bonte E, Krijgsveld J, Katsantoni E, Heck A, Grosveld F, et al. (2003) Efficient biotinylation and single-step purification of tagged transcription factors in mammalian cells and transgenic mice. Proc Natl Acad Sci USA 100:7480-7485
6. Duffy S, Tsao KL, Waugh DS (1998) Site-specific, enzymatic biotinylation of recombinant proteins in Spodoptera frugiperda cells using biotin acceptor peptides. Anal Biochem 262:122-128
7. Furuyama T, Henikoff S (2006) Biotin-tag affinity purification of a centromeric nucleosome assembly complex. Cell Cycle 5:1269-1274
8. Hollinshead M, Sanderson J, Vaux DJ (1997) Anti-biotin antibodies offer superior organelle-specific labeling of mitochondria over avidin or streptavidin. J Histochem Cytochem 45:1053-1058
9. Jarvik JW, Telmer CA (1998) Epitope tagging. Annu Rev Genet 32:601-618
10. Nakatani Y, Ogryzko V (2003) Immunoaffinity purification of mammalian protein complexes. Methods Enzymol 370:430-444
11. Parrott MB, Barry MA (2000) Metabolic biotinylation of recombinant proteins in mammalian cells and in mice. Mol Ther 1:96-104
12. Parrott MB, Barry MA (2001) Metabolic biotinylation of secreted and cell surface proteins from mammalian cells. Biochem Biophys Res Commun 281:993-1000
13. Prasad PD, Wang H, Kekuda R, Fujita T, Fei YJ, Devoe LD, Leibach FH, et al. (1998) Cloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoate. J Biol Chem 273:7501-7506
14. Roth J (1989) Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components. In Tartakoff AM, ed. Methods of Cell Biology. New York, Academic Press, 513-551
15. Schatz PJ (1993) Use of peptide libraries to map the substrate specificity of a peptide-modifying enzyme: a 13 residue consensus peptide specifies biotinylation in Escherichia coli. Biotechnology (NY) 11:1138-1143
16. Seeler JS, Dejean A (2003) Nuclear and unclear functions of SUMO. Nat Rev Mol Cell Biol 4:690-699
17. Smith PA, Tripp BC, DiBlasio-Smith EA, Lu Z, La Value ER, McCoy JM (1998) A plasmid expression system for quantitative in vivo biotinylation of thioredoxin fusion proteins in Escherichia coli. Nucleic Acids Res 26:1414-1420
18. van Werven FJ, Timmers HT (2006) The use of biotin tagging in Saccharomyces cerevisiae improves the sensitivity of chromatin immunoprecipitation. Nucleic Acids Res 34:e33
19. Viens A, Mechold U, Lehrmann H, Harel-Bellan A, Ogryzko V (2004) Use of protein biotinylation in vivo for chromatin immunoprecipitation. Anal Biochem 325:68-76
20. Visa N, Puvion-Dutilleul F, Harper F, Bachellerie JP, Puvion E (1993) Intranuclear distribution of poly(A) RNA determined by electron microscope in situ hybridization. Exp Cell Res 208:19-34