Structural determinants for rCNT2 sorting to the plasma membrane of polarized and non-polarized cells

Biochemical Journal

Volumn 442, Issue 3, 2012, Pages 517-525

  Pinilla Macua, Itziar ^a   Casado, F Javier ^a   Pastor Anglada, Marçal ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Concentrative nucleoside transporter 2 (CNT2); Epithelium; Phosphorylation; Plasma membrane; Protein kinase CK2; Sorting

Indexed keywords

2 DIMETHYLAMINO 4,5,6,7 TETRABROMO 1H BENZIMIDAZOLE; CASEIN KINASE II; CONCENTRATIVE NUCLEOSIDE TRANSPORTER 2; GLUTAMIC ACID; NUCLEOSIDE TRANSPORTER; PROTEIN SERINE THREONINE KINASE INHIBITOR; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BRUSH BORDER; CELL MEMBRANE; CELL MIGRATION; CELL SELECTION; CELL STRUCTURE; CELL SURFACE; CONTROLLED STUDY; ENDOCYTOSIS; MEMBRANE POLARIZATION AND DEPOLARIZATION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN STABILITY; PROTEIN STRUCTURE;

ANIMALS; BENZIMIDAZOLES; CELL MEMBRANE; CELL POLARITY; CELLS, CULTURED; CHO CELLS; CRICETINAE; DOGS; MEMBRANE TRANSPORT PROTEINS; PROTEIN TRANSPORT; RATS; TRANSFECTION;

RATTUS;

EID: 84857893793     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20110605     Document Type: Article

References (38)

1. Soler, C., Garcia-Manteiga, J., Valdes, R., Xaus, J., Comalada, M., Casado, F.J., Pastor-Anglada, M., Celada, A. and Felipe, A. (2001) Macrophages require different nucleoside transport systems for proliferation and activation. FASEB J. 15, 1979-1988 (Pubitemid 32848802)
2. Soler, C., Valdes, R., Garcia-Manteiga, J., Xaus, J., Comalada, M., Casado, F. J., Modolell, M., Nicholson, B., Macleod, C., Felipe, A. et al. (2001) Lipopolysaccharide-induced apoptosis of macrophages determines the up-regulation of concentrative nucleoside transporters Cnt1 and Cnt2 through tumor necrosis factor-α-dependent and -independent mechanisms. J. Biol. Chem. 276, 30043-30049
3. Valdes, R., Ortega, M. A., Casado, F. J., Felipe, A., Gil, A., Sanchez-Pozo, A. and Pastor-Anglada, M. (2000) Nutritional regulation of nucleoside transporter expression in rat small intestine. Gastroenterology 119, 1623-1630 (Pubitemid 32198687)
4. Duflot, S., Calvo, M., Casado, F. J., Enrich, C. and Pastor-Anglada, M. (2002) Concentrative nucleoside transporter (rCNT1) is targeted to the apical membrane through the hepatic transcytotic pathway. Exp. Cell Res. 281, 77-85 (Pubitemid 35373784)
5. Govindarajan, R., Endres, C. J., Whittington, D., Lecluyse, E., Pastor-Anglada, M., Tse, C. M. and Unadkat, J. D. (2008) Expression and hepatobiliary transport characteristics of the concentrative and equilibrative nucleoside transporters in sandwich-cultured human hepatocytes. Am. J. Physiol. Gastrointest. Liver Physiol. 295, G570-G580
6. Mangravite, L. M., Badagnani, I. and Giacomini, K. M. (2003) Nucleoside transporters in the disposition and targeting of nucleoside analogs in the kidney. Eur. J. Pharmacol. 479, 269-281 (Pubitemid 37377895)
7. Lai, Y., Bakken, A. H. and Unadkat, J. D. (2002) Simultaneous expression of hCNT1-CFP and hENT1-YFP in Madin-Darby canine kidney cells: localization and vectorial transport studies. J. Biol. Chem. 277, 37711-37717
8. Errasti-Murugarren, E., Pastor-Anglada, M. and Casado, F. J. (2007) Role of CNT3 in the transepithelial flux of nucleosides and nucleoside-derived drugs. J. Physiol. 582, 1249-1260 (Pubitemid 47104352)
9. Rodriguez-Mulero, S., Errasti-Murugarren, E., Ballarin, J., Felipe, A., Doucet, A., Casado, F. J. and Pastor-Anglada, M. (2005) Expression of concentrative nucleoside transporters SLC28 (CNT1, CNT2, and CNT3) along the rat nephron: effect of diabetes. Kidney Int. 68, 665-672 (Pubitemid 43323121)
10. Vitzthum, H., Weiss, B., Bachleitner, W., Kramer, B. K. and Kurtz, A. (2004) Gene expression of adenosine receptors along the nephron. Kidney Int. 65, 1180-1190 (Pubitemid 38406956)
11. Fernandez-Veledo, S., Huber-Ruano, I., Aymerich, I., Duflot, S., Casado, F. J. and Pastor-Anglada, M. (2006) Bile acids alter the subcellular localization of CNT2 (concentrative nucleoside cotransporter) and increase CNT2-related transport activity in liver parenchymal cells. Biochem. J. 395, 337-344
12. Aymerich, I., Foufelle, F., Ferré, P., Casado, F. J. and Pastor-Anglada, M. (2006) Extracellular adenosine activates AMP-dependent protein kinase (AMPK). J. Cell Sci. 119, 1612-1621 (Pubitemid 43732987)
13. Huber-Ruano, I., Pinilla-Macua, I., Torres, G., Casado, F. J. and Pastor-Anglada, M. (2010) Link between high-affinity adenosine concentrative nucleoside transporter-2 (CNT2) and energy metabolism in intestinal and liver parenchymal cells. J. Cell. Physiol. 225, 620-630
14. Aymerich, I., Pastor-Anglada, M. and Casado, F. J. (2004) Long term endocrine regulation of nucleoside transporters in rat intestinal epithelial cells. J. Gen. Physiol. 124, 505-512 (Pubitemid 39506286)
15. Del Santo, B., Valdes, R., Mata, J., Felipe, A., Casado, F. J. and Pastor-Anglada, M. (1998) Differential expression and regulation of nucleoside transport systems in rat liver parenchymal and hepatoma cells. Hepatology 28, 1504-1511 (Pubitemid 28549119)
16. Errasti-Murugarren, E., Molina-Arcas, M., Casado, F. J. and Pastor-Anglada, M. (2009) A splice variant of the SLC28A3 gene encodes a novel human concentrative nucleoside transporter-3 (hCNT3) protein localized in the endoplasmic reticulum. FASEB J. 23, 172-182
17. Sun, A. Q., Salkar, R., Sachchidanand, R., Xu, S., Zeng, L., Zhou, M. M. and Suchy, F. J. (2003) A 14-amino acid sequence with a β-turn structure is required for apical membrane sorting of the rat ileal bile acid transporter. J. Biol. Chem. 278, 4000-4009 (Pubitemid 36801132)
18. Ahmed, K., Gerber, D. A. and Cochet, C. (2002) Joining the cell survival squad: an emerging role for protein kinase CK2. Trends Cell Biol. 12, 226-230 (Pubitemid 34327615)
19. Lim, A. C., Tiu, S. Y., Li, Q. and Qi, R. Z. (2004) Direct regulation of microtubule dynamics by protein kinase CK2. J. Biol. Chem. 279, 4433-4439 (Pubitemid 38199032)
20. Niefind, K., Raaf, J. and Issinger, O. G. (2009) Protein kinase CK2 in health and disease: protein kinase CK2: from structures to insights. Cell. Mol. Life Sci. 66, 1800-1816
21. Roosbeek, S., Peelman, F., Verhee, A., Labeur, C., Caster, H., Lensink, M. F., Cirulli, C., Grooten, J., Cochet, C., Vandekerckhove, J. et al. (2004) Phosphorylation by protein kinase CK2 modulates the activity of the ATP binding cassette A1 transporter. J. Biol. Chem. 279, 37779-37788 (Pubitemid 39195492)
22. Bone, D. B., Robillard, K. R., Stolk, M. and Hammond, J. R. (2007) Differential regulation of mouse equilibrative nucleoside transporter 1 (mENT1) splice variants by protein kinase CK2. Mol. Membr. Biol. 24, 294-303 (Pubitemid 46801847)
23. +exchanger 3 (NHE3) and stimulates NHE3 basal activity by phosphorylating a separate site in NHE3. Mol. Biol. Cell 19, 3859-3870
24. Harris, M. J., Kagawa, T., Dawson, P. A. and Arias, I. M. (2004) Taurocholate transport by hepatic and intestinal bile acid transporters is independent of FIC1 overexpression in Madin-Darby canine kidney cells. J. Gastroenterol. Hepatol. 19, 819-825 (Pubitemid 38981406)
25. + nucleoside transporter CNT2 cloned from rat blood-brain barrier. J. Pharmacol. Exp. Ther. 299, 735-740
26. Errasti-Murugarren, E., Casado, F. J. and Pastor-Anglada, M. (2010) Different N-terminal motifs determine plasma membrane targeting of the human concentrative nucleoside transporter 3 in polarized and nonpolarized cells. Mol. Pharmacol. 78, 795-803
27. Mangravite, L. M. and Giacomini, K. M. (2003) Sorting of rat SPNT in renal epithelium is independent of N-glycosylation. Pharm. Res. 20, 319-323 (Pubitemid 36277685)
28. Scott, G. K., Fei, H., Thomas, L., Medigeshi, G. R. and Thomas, G. (2006) A PACS-1, GGA3 and CK2 complex regulates CI-MPR trafficking. EMBO J. 25, 4423-4435 (Pubitemid 44498117)
29. Shi, X., Potvin, B., Huang, T., Hilgard, P., Spray, D. C., Suadicani, S. O., Wolkoff, A. W., Stanley, P. and Stockert, R. J. (2001) A novel casein kinase 2 α-subunit regulates membrane protein traffic in the human hepatoma cell line HuH-7. J. Biol. Chem. 276, 2075-2082 (Pubitemid 32109689)
30. Bonifacino, J. S. and Traub, L. M. (2003) Signals for sorting of transmembrane proteins to endosomes and lysosomes. Annu. Rev. Biochem. 72, 395-447 (Pubitemid 36930451)
31. Owen, R. P., Gray, J. H., Taylor, T. R., Carlson, E. J., Huang, C. C., Kawamoto, M., Johns, S. J., Stryke, D., Ferrin, T. E. and Giacomini, K. M. (2005) Genetic analysis and functional characterization of polymorphisms in the human concentrative nucleoside transporter, CNT2. Pharmacogenet. Genomics 15, 83-90 (Pubitemid 40562985)
32. Kirchhausen, T., Macia, E. and Pelish, H. E. (2008) Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis. Methods Enzymol. 438, 77-93
33. Wolff, S. C., Qi, A. D., Harden, T. K. and Nicholas, R. A. (2010) Charged residues in the C-terminus of the P2Y1 receptor constitute a basolateral-sorting signal. J. Cell Sci. 123, 2512-2520
34. 1 adenosine receptors. Mol. Cell. Biol. 24, 2710-2719
35. Zuzarte, M., Rinne, S., Schlichthorl, G., Schubert, A., Daut, J. and Preisig-Muller, R. (2007) A di-acidic sequence motif enhances the surface expression of the potassium channel TASK-3. Traffic 8, 1093-1100 (Pubitemid 47074041)
36. Andersen, M. N., Olesen, S. P. and Rasmussen, H. B. (2011) Kv7.1 surface expression is regulated by epithelial cell polarization. Am. J. Physiol. Cell. Physiol. 300, C814-C824
37. Krasnov, K. V., Tzetis, M., Cheng, J., Guggino, W. B. and Cutting, G. R. (2008) Localization studies of rare missense mutations in cystic fibrosis transmembrane conductance regulator (CFTR) facilitate interpretation of genotype-phenotype relationships. Hum. Mutat. 29, 1364-1372
38. Andersen, M. N., Krzystanek, K., Jespersen, T., Olesen, S. P. and Rasmussen, H. B. (2012) AMP-activated protein kinase downregulates Kv7.1 cell surface expression. Traffic 13, 143-156