Efficient expression of a Paenibacillus barcinonensis endoglucanase in Saccharomyces cerevisiae

Journal of Industrial Microbiology and Biotechnology

Volumn 39, Issue 1, 2012, Pages 115-123

  Mormeneo, María ^a   Pastor, Fi Javier ^b   Zueco, Jesús ^a  

^a UNIVERSITY OF VALENCIA   (Spain)

^b UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Endoglucanase; Paenibacillus barcinonensis; Pir4 cell wall protein; Saccharomyces cerevisiae; Secretion of recombinant proteins

Indexed keywords

CARBOXYMETHYLCELLULOSE; CELL MEMBRANE PROTEIN; GLUCAN SYNTHASE; HYBRID PROTEIN; PROTEIN PIR4; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME LOCALIZATION; GLYCOSYLATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS; PAENIBACILLUS BARCINONENSIS; PROTEIN EXPRESSION; PROTEIN SECRETION; SACCHAROMYCES CEREVISIAE;

BACTERIAL PROTEINS; BATCH CELL CULTURE TECHNIQUES; CELLULASE; GLYCOSYLATION; PAENIBACILLUS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

PAENIBACILLUS BARCINONENSIS; SACCHAROMYCES CEREVISIAE;

EID: 84857630415     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-011-1006-8     Document Type: Article

References (65)

1. Andrés I, Zueco J, Parascandola P (2003) Immobilization of Saccharomyces cerevisiae cells to protein G-sepharose by cell wall engineering. J Mol Microbiol Biotechnol 5:161-166 (Pubitemid 37475745)
2. Andrés I, Gallardo O, Parascandola P, Pastor FIJ, Zueco J (2005) Use of cell wall protein Pir4 as a fusion partner for the expression of Bacillus sp. BP-7 xylanase A in Saccharomyces cerevisiae. Biotech Bioeng 89:690-697 (Pubitemid 40470357)
3. Andrés I, Rodríguez-Díaz J, Buesa J, Zueco J (2006) Yeast expression of the VP8* fragment of the rotavirus spike protein and its use as immunogen in mice. Biotechnol Bioeng 93:89-98 (Pubitemid 43117062)
4. Bamforth C (1994) b-glucan and b-glucanases in malting and brewing: practical aspects. Brew Dig 69:12-16
5. Bayer EA, Shoham Y, Lamed R (2006) Cellulose decomposing bacteria and their enzyme systems. In: Dworkin M, Falkow S, Rosenberg E, Schleifer KH, Stackebrandt E (eds) The Prokaryotes, vol 2. Springer, Berlin Heidelberg New York, pp 578-617
6. Béguin P, Aubert JP (1994) The biological degradation of cellulose. FEMS Microbiol Rev 13:25-58 (Pubitemid 24051006)
7. Benitez J, Silva A, Vazquez R, Noa MD, Hollenberg CP (1989) Secretion and glycosylation of Clostridium thermocellum endoglucanase A encoded by the celA gene in Saccharomyces cerevisiae. Yeast 5:299-306
8. Blanco A, Vidal T, Colom JF, Pastor FI (1995) Purification and properties of xylanase A from alkali-tolerant Bacillus sp. strain BP-23. Appl Environ Microbiol 61:4468-4470
9. Blanco A, Díaz P, Martínez J, Vidal T, Torres AL, Pastor FI (1998) Cloning of a new endoglucanase gene from Bacillus sp. BP-23 and characterisation of the enzyme. Performance in paper manufacture from cereal straw. Appl Microbiol Biotechnol 50:48-54 (Pubitemid 28372375)
10. Blanco A, Díaz P, Zueco J, Parascandola P, Javier Pastor FI (1999) A multidomain xylanase from a Bacillus sp. with a region homologous to thermostabilizing domains of thermophilic enzymes. Microbiology 145:2163-2170 (Pubitemid 29396947)
11. Burnette WN (1981) Western blotting: electrophoretic transfer of proteins from sodium dodecyl-sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195-203
12. Cebollero E, Gonzalez-Ramos D, Tabera L, Gonzalez R (2007) Transgenic wine yeast technology comes of age: is it time for transgenic wine? Biotechnol Lett 29:191-200 (Pubitemid 46089470)
13. Chiriac AI, Cadena EM, Vidal T, Torres AL, Diaz P, Pastor FI (2010) Engineering a family 9 processive endoglucanase from Paenibacillus barcinonensis displaying a novel architecture. Appl Microbiol Biotechnol 86:1125-1134
14. Cho KM, Yoo YJ (1999) Novel SSF process for ethanol production from microcrystalline cellulose using the d-integrated recombinant yeast, Saccharomyces cerevisiae L2612dGC. J Microbiol Biotechnol 9:340-345 (Pubitemid 29327421)
15. Den Haan R, Rose SH, Lynd LR, van Zyl WH (2007) Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae. Metab Eng 9:87-94 (Pubitemid 44960875)
16. Plessis Du, Rose SH, van Zyl H (2010) Exploring improved endoglucanase expression in Saccharomyces cerevisiae strains. Appl Microbiol Biotechnol 86:1503-1511
17. Fujita Y, Takahashi S, Ueda M, Tanaka A, Okada H, Morikawa Y, Kawaguchi T, Arai M, Fukuda H, Kondo A (2002) Direct and efficient production of ethanol from cellulosic material with a yeast strain displaying cellulolytic enzymes. Appl Environ Microbiol 68:5136-5141 (Pubitemid 35154825)
18. Fujita Y, Ito J, Ueda M, Fukuda H, Kondo A (2004) Synergistic saccharification, and direct fermentation to ethanol, of amorphous cellulose by use of an engineered yeast strain codisplaying three types of cellulolytic enzyme.Appl EnvironMicrobiol 70:1207-1212 (Pubitemid 38568250)
19. Gai SA, Wittrup KD (2007) Yeast surface display for protein engineering and characterization. Curr Opin Struct Biol 17. 467-473 (Pubitemid 47454774)
20. Gietz RD, Sugino A (1988) New yeast-Escherichia coli shuttle vector constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74:527-534 (Pubitemid 19060666)
21. Guo Q, Zhang W, Ma L, Chen Q, Chen J, Zhang H, Ruan H, He G (2010) A food-grade industrial arming yeast expressing b-1, 3-1, 4-glucanase with enhanced thermal stability. J Zhejiang Univ Sci B 11:41-51
22. Hernandez LM, Ballou L, Alvarado E, Gillece-Castro BL, Burlingame AL, Ballou CE (1989) A new Saccharomyces cerevisiae mnn mutant N-linked oligosaccharide structure. J Biol Chem 246:11846-11856
23. Hinchliffe E, Box WG (1984) Expression of the cloned endo-1, 3-1, 4-b-glucanase gene of Bacillus subtilis in Saccharomyces cerevisiae. Curr Genet 8:471-475 (Pubitemid 14068469)
24. Hyeon JE, Yu KO, Suh DJ, Suh YW, Lee SE, Lee J, Han SO (2010) Production of minicellulosomes from Clostridium cellulovorans for the fermentation of cellulosic ethanol using engineered recombinant Saccharomyces cerevisiae. FEMS Microbiol Lett 310:39-47
25. Ito H, Fukuda Y, Murata K, Kimura A (1983) Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163-168 (Pubitemid 13119852)
26. Kotaka A, Sahara H, Hata Y, Abe Y, Kondo A, Kato-Murai M, Kuroda K, Ueda M (2008) Efficient and direct fermentation of starch to ethanol by sake yeast strains displaying fungal glucoamylases. Biosci Biotechnol Biochem 72:1376-1379 (Pubitemid 351872508)
27. Laemmli UK (1970) Cleavage of structural proteins during the head assembly of bacteriophage T4. Nature 227:680-685
28. Lee JH, Lim MY, Kim MJ, Heo SY, Seo JH, Kim YH, Nam SW (2007) Constitutive coexpression of Bacillus endoxylanase and Trichoderma endoglucanase genes in Saccharomyces cerevisiae. J Microbiol Biotechnol 17:2076-2080
29. Lilly M, Fierobe HP, van Zyl WH, Volschenk H (2009) Heterologous expression of a Clostridium minicellulosome in Saccharomyces cerevisiae. FEMS Yeast Res 9:1236-1249
30. Lin Y, Tanaka S (2006) Ethanol fermentation from biomass resources: current state and prospects. Appl Microbiol Biotechnol 69:627-642 (Pubitemid 43136522)
31. Louw C, La Grange D, Pretorius IS, van Rensburg P (2006) The effect of polysaccharide-degrading wine yeast transformants on the efficiency of wine processing and wine flavour. J Biotechnol 125:447-461 (Pubitemid 44333122)
32. Lu Y, Wang TH, Ding XL (2009) Induction of production and secretion b(1-4) glucanase with Saccharomyces cerevisiae by replacing the MET10 gene with egl1 gene from Trichoderma reesei. Letters Appl Microbiol 49:702-707
33. Lynd LR, Weimer PJ, van Zyl WH, Pretorius IS (2002) Microbial cellulose utilization: fundamentals and biotechnology. Microbiol Mol Biol Rev 66:506-577 (Pubitemid 35005827)
34. Lynd LR, van Zyl WH, McBride JE, Laser M (2005) Consolidated bioprocessing of cellulosic biomass: an update. Curr Opin Biotechnol 16:577-583 (Pubitemid 41393839)
35. Lynd LR, Laser MS, Bransby D, Dale BE, Davison B, Hamilton R, Himmel M, Keller M, McMillan JD, Sheehan J, Wyman CE (2008) How biotech can transform biofuels. Nat Biotechnol 26:169-172 (Pubitemid 351225943)
36. Mormeneo M, Andrés I, Bofill C, Díaz P, Zueco J (2008) Efficient secretion of Bacillus subtilis lipase A in Saccharomyces cerevisiae by translational fusion to the Pir4 cell wall protein. Appl Microbiol Biotechnol 80:437-445
37. Moukadiri I, Jaafar L, Zueco J (1999) Identification of two mannoproteins released from cell walls of a Saccharomyces cerevisiae mnn1 mnn9 double mutant by reducing agents. J Bacteriol 181:4741-4745 (Pubitemid 29383498)
38. Moukadiri I, Zueco J (2001) Evidence for the attachment of Hsp150/Pir2 to the cell wall of Saccharomyces cerevisiae through disulfide bridges. FEMS Yeast Res 1:241-245 (Pubitemid 34442274)
39. Murai T, Ueda M, Atomi H, Shibasaki Y, Kamasawa N, Osumi M, Kawaguchi T, Arai M, Tanaka A (1997) Genetic immobilization of cellulase on the cell surface of Saccharomyces cerevisiae. Appl Microbiol Biotechnol 48:499-503 (Pubitemid 27475288)
40. Paciello L, de Alteriis E, Mazzoni C, Palermo V, Zueco J, Parascandola P (2009) Performance of the auxotrophic Saccharomyces cerevisiae BY4741 as host for the production of IL-1b in aerated fed-batch reactor: role of ACA supplementation, strain viability and maintenance energy. Microbial Cell Factories 8:70
41. Paciello L, Andrés I, Zueco J, Bianchi MM, de Alteriis E, Parascandola P (2010) Expression of human interleukin-1b in Saccharomyces cerevisiae using PIR4 as fusion partner and production in aereated fed-batch reactor. Ann Microbiol 60:719-72.
42. Park S, Xu Y, Stowell XF, Gai F, Saven JG, Boder ET (2006) Limitations of yeast surface display in engineering proteins of high thermostability. Protein Eng Des Sel 19:211-217
43. Pastor FI, Pujol X, Blanco A, Vidal T, Torres AL, Díaz P (2001) Molecular cloning and characterization of a multidomain endoglucanase from Paenibacillus sp BP-23: evaluation of its performance in pulp refining. Appl Microbiol Biotechnol 55:61-68 (Pubitemid 32206372)
44. Picart P, Diaz P, Pastor FI (2007) Cellulases from two Penicillium sp. strains isolated from subtropical forest soil: production and characterization. Lett Appl Microbiol 45:108-113 (Pubitemid 47012112)
45. Qin Y, Wei X, Liu X, Wang T, Qu Y (2008) Purification and characterization of recombinant endoglucanase of Trichoderma reesei expressed in Saccharomyces cerevisiae with higher glycosylation and stability. Protein Expr Purif 58:162-167
46. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
47. Sánchez MM, Pastor FI, Diaz P (2003) Exo-mode of action of cellobiohydrolase Cel48C from Paenibacillus sp. BP-23. A unique type of cellulase among Bacillales. Eur J Biochem 270:2913-2919 (Pubitemid 36801832)
48. Sánchez MM, Fritze D, Blanco A, Spröer C, Tindall BJ, Schumann P, Kroppenstedt RM, Diaz P, Pastor FI (2005) Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55:935-939 (Pubitemid 40485093)
49. Schuller D, Casal M (2005) The use of genetically modified Saccharomyces cerevisiae strains in the wine industry. Appl Microbiol Biotechnol 68:292-304 (Pubitemid 41159999)
50. Shusta EV, Kieke MC, Parke E, Kranz DM, Wittrup KD (1999) Yeast polypeptide fusion surface display levels predict thermal stability and soluble secretion efficiency. J Mol Biol 292:949-956 (Pubitemid 29481708)
51. Skipper N, Sutherland M, Davies RW, Kilburn D, Miller RC Jr, Warren A, Wong R (1985) Secretion of a bacterial cellulase by yeast. Science 230:958-960 (Pubitemid 16193995)
52. Spiro RG (1966) The Nelson-Somogyi copper reduction method. Analysis of sugars found in glycoprotein. Method Enzymol 8:3-2.
53. Strauss MLA, Jolly NP, Lambechts MG, van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeast. J Appl Microbiol 91:182-190 (Pubitemid 32623365)
54. Tanino T, Fukuda H, Kondo A (2006) Construction of a Pichia pastoris cell-surface display system using Flo1p anchor system. Biotechnol Progr 22:989-993 (Pubitemid 44230686)
55. Teather RM, Wood PJ (1982) Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43:777-78.
56. Teeri TT (1997) Crystalline cellulose degradation: new insight into the function of cellobiohydrolase. Trends Biotechnol 15:160-167 (Pubitemid 27219573)
57. Toda H, Takada S, Oda M, Amano Y, Kanda T, Okazaki M, Shimosaka M (2005) Gene cloning of an endoglucanase from the basidiomycete Irpex lacteus and its cDNA expression in Saccharomyces cerevisiae. Biosci Biotechnol Biochem 69:1262-1269 (Pubitemid 41185206)
58. Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76. 4350-4354 (Pubitemid 9256559)
59. van Rensburg P, van Zyl WH, Pretorius IS (1994) Expression of the Butyrivibrio fibrisolvens endo-beta-1, 4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae. Curr Genet 27:17-22 (Pubitemid 24368399)
60. van Rensburg P, van Zyl WH, Pretorius IS (1996) Co-expression of a Phanerochaete chrysosporium cellobiohydrolase gene and a Butyrivibrio fibrisolvens endo-beta-1, 4-glucanase gene in Saccharomyces cerevisiae. Curr Genet 30:246-250 (Pubitemid 26336190)
61. van Rensburg P, Strauss ML, Lambrechts MG, Cordero Otero RR, Pretorius IS (2007) The heterologous expression of polysaccharidase- encoding genes with oenological relevance in Saccharomyces cerevisiae. J Appl Microbiol 103:2248-2257 (Pubitemid 350193194)
62. van Zyl WH, Lynd LR, den Haan R, McBride JE (2007) Consolidated bioprocessing for bioethanol production using Saccharomyces cerevisiae. Adv Biochem Eng Biotechnol 108:205-235 (Pubitemid 47434547)
63. Wen F, Sun J, Zhao H (2010) Yeast surface display of trifunctional minicellulosomes for simultaneous saccharification and fermentation of cellulose to ethanol. Appl Environ Microbiol 76:1251-1260
64. Wyman CE (2007) What is (and is not) vital to advancing cellulosic ethanol. Trends Biotechnol 25:153-157 (Pubitemid 46413369)
65. Zhu H, Yao S, Wang S (2010) MF a signal peptide enhances the expression of cellulase eg1 gene in yeast. Appl Biochem Biotechnol 162:617-624