Molecular diagnosis of cutaneous leishmaniasis and species identification: Analysis of 122 biopsies with varied parasite index

Journal of Cutaneous Pathology

Volumn 39, Issue 3, 2012, Pages 347-355

  Yehia, Lamis ^a   Adib Houreih, Mohammad ^b   Raslan, Wasim Fawzi ^c   Kibbi, Abdul Ghani ^a   Loya, Asif ^d   Firooz, Alireza ^e   Satti, Mohamed ^f   El Sabban, Marwan ^a   Khalifeh, Ibrahim ^a  

^a AMERICAN UNIVERSITY OF BEIRUT   (Lebanon)

^b TISHREEN UNIVERSITY   (Syrian Arab Republic)

^c Department of Pathology   (Saudi Arabia)

^d Shaukat Khanum Memorial Cancer Center   (Pakistan)

^e TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^f KING SAUD BIN ABDULAZIZ UNIVERSITY FOR HEALTH SCIENCES   (Saudi Arabia)

Author keywords

cutaneous leishmaniasis; Leishmania ribosomal internal transcribed spacer 1 polymerase chain reaction; restriction fragment length polymorphism

Indexed keywords

PROTOZOAL DNA; RIBOSOMAL SPACER DNA;

ADOLESCENT; ADULT; AGED; ARTICLE; CHILD; CLINICAL TRIAL; FEMALE; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LEISHMANIA; MALE; METHODOLOGY; MIDDLE AGED; MIDDLE EAST; MULTICENTER STUDY; PARASITOLOGY; PATHOLOGY; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; SKIN; SKIN LEISHMANIASIS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; CHILD; CHILD, PRESCHOOL; DNA, PROTOZOAN; DNA, RIBOSOMAL SPACER; FEMALE; HUMANS; LEISHMANIA; LEISHMANIASIS, CUTANEOUS; MALE; MIDDLE AGED; MIDDLE EAST; POLYMERASE CHAIN REACTION; SKIN;

EID: 84857460120     PISSN: 03036987     EISSN: 16000560     Source Type: Journal    
DOI: 10.1111/j.1600-0560.2011.01861.x     Document Type: Article

References (37)

1. Schonian G, Nasereddin A, Dinse N, et al., PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 2003; 47: 349.
2. Goto H, Lindoso JA., Current diagnosis and treatment of cutaneous and mucocutaneous leishmaniasis. Expert Rev Anti Infect Ther 2010; 8: 419.
3. Scarisbrick JJ, Chiodini PL, Watson J, et al., Clinical features and diagnosis of 42 travellers with cutaneous leishmaniasis. Travel Med Infect Dis 2006; 4: 14.
4. Ameen M., Cutaneous leishmaniasis: advances in disease pathogenesis, diagnostics and therapeutics. Clin Exp Dermatol 2010; 35: 699.
5. Salman SM, Rubeiz NG, Kibbi AG., Cutaneous leishmaniasis: clinical features and diagnosis. Clin Dermatol 1999; 17: 291.
6. David CV, Craft N., Cutaneous and mucocutaneous leishmaniasis. Dermatol Ther 2009; 22: 491.
7. Singh S, Dey A, Sivakumar R., Applications of molecular methods for Leishmania control. Expert Rev Mol Diagn 2005; 5: 251.
8. Farah FS, Malak JA., Cutaneous leishmaniasis. Arch Dermatol 1971; 103: 467.
9. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL., Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 2006; 44: 1435.
10. Safaei A, Motazedian MH, Vasei M., Polymerase chain reaction for diagnosis of cutaneous leishmaniasis in histologically positive, suspicious and negative skin biopsies. Dermatology 2002; 205: 18.
11. Schonian G, Mauricio I, Gramiccia M, Canavate C, Boelaert M, Dujardin JC., Leishmaniases in the Mediterranean in the era of molecular epidemiology. Trends Parasitol 2008; 24: 135.
12. Ridley DS, Ridley MJ., The evolution of the lesion in cutaneous leishmaniasis. J Pathol 1983; 141: 83.
13. el Tai NO, Osman OF, el Fari M, Presber W, Schonian G., Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg 2000; 94: 575.
14. Kazemi-Rad E., Diagnosis and characterization of Leishmania species in giemsa-stained slides by PCR-RFLP. Iran J Public Health 2008; 37: 54.
15. Barker DC., Molecular approaches to DNA diagnosis. Parasitology 1989; 99 (Suppl): S125.
16. Laskay T, Miko TL, Negesse Y, Solbach W, Rollinghoff M, Frommel D., Detection of cutaneous Leishmania infection in paraffin-embedded skin biopsies using the polymerase chain reaction. Trans R Soc Trop Med Hyg 1995; 89: 273.
17. Meredith SE, Zijlstra EE, Schoone GJ, et al., Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material. Arch Inst Pasteur Tunis 1993; 70: 419.
18. Osman OF, Oskam L, Zijlstra EE, et al., Evaluation of PCR for diagnosis of visceral leishmaniasis. J Clin Microbiol 1997; 35: 2454.
19. Boggild AK, Valencia BM, Espinosa D, et al., Detection and species identification of Leishmania DNA from filter paper lesion impressions for patients with American cutaneous leishmaniasis. Clin Infect Dis 2010; 50: e1.
20. van Eys GJ, Schoone GJ, Kroon NC, Ebeling SB., Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol 1992; 51: 133.
21. Mugasa CM, Laurent T, Schoone GJ, et al., Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis. Parasit Vectors 2010; 3: 13.
22. Minodier P, Piarroux R, Gambarelli F, Joblet C, Dumon H., Rapid identification of causative species in patients with Old World leishmaniasis. J Clin Microbiol 1997; 35: 2551.
23. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J., Rapid and simple method for purification of nucleic acids. J Clin Microbiol 1990; 28: 495.
24. Spanakos G, Piperaki ET, Menounos PG, Tegos N, Flemetakis A, Vakalis NC., Detection and species identification of Old World Leishmania in clinical samples using a PCR-based method. Trans R Soc Trop Med Hyg 2008; 102: 46.
25. Goelz SE, Hamilton SR, Vogelstein B., Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Commun 1985; 130: 118.
26. Shibata DK, Arnheim N, Martin WJ., Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction. J Exp Med 1988; 167: 225.
27. Huijsmans CJ, Damen J, van der Linden JC, Savelkoul PH, Hermans MH., Comparative analysis of four methods to extract DNA from paraffin-embedded tissues: effect on downstream molecular applications. BMC Res Notes 2010; 3: 239.
28. Momeni AZ, Yotsumoto S, Mehregan DR, et al., Chronic lupoid leishmaniasis. Evaluation by polymerase chain reaction. Arch Dermatol 1996; 132: 198.
29. Vega-Lopez F., Diagnosis of cutaneous leishmaniasis. Curr Opin Infect Dis 2003; 16: 97.
30. Al-Jawabreh A, Schoenian G, Hamarsheh O, Presber W., Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of giemsa-stained smears. Acta Trop 2006; 99: 55.
31. Schallig HD, Oskam L., Molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification. Trop Med Int Health 2002; 7: 641.
32. El Tai NO, El Fari M, Mauricio I, et al., Leishmania donovani: intraspecific polymorphisms of Sudanese isolates revealed by PCR-based analyses and DNA sequencing. Exp Parasitol 2001; 97: 35.
33. Nasereddin A, Bensoussan-Hermano E, Schonian G, Baneth G, Jaffe CL., Molecular diagnosis of Old World cutaneous leishmaniasis and species identification by use of a reverse line blot hybridization assay. J Clin Microbiol 2008; 46: 2848.
34. Azmi K, Nasereddin A, Ereqat S, Schonian G, Abdeen Z., Identification of Old World Leishmania species by PCR-RFLP of the 7 spliced leader RNA gene and reverse dot blot assay. Trop Med Int Health 2010; 15: 872.
35. Cupolillo E, Grimaldi Junior G, Momen H, Beverley SM., Intergenic region typing (IRT): a rapid molecular approach to the characterization and evolution of Leishmania. Mol Biochem Parasitol 1995; 73: 145.
36. Castilho TM, Shaw JJ, Floeter-Winter LM., New PCR assay using glucose-6-phosphate dehydrogenase for identification of Leishmania species. J Clin Microbiol 2003; 41: 540.
37. Rodgers MR, Popper SJ, Wirth DF., Amplification of kinetoplast DNA as a tool in the detection and diagnosis of Leishmania. Exp Parasitol 1990; 71: 267.