A Chloroflexi bacterium dechlorinates polychlorinated biphenyls in marine sediments under in situ-like biogeochemical conditions

Journal of Hazardous Materials

Volumn 209-210, Issue , 2012, Pages 449-457

  Zanaroli, Giulio ^a   Balloi, Annalisa ^b   Negroni, Andrea ^a,c   Borruso, Luigimaria ^b   Daffonchio, Daniele ^b   Fava, Fabio ^a  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

^c ENVIREN (The cluster of environmental laboratories of the Emilia Romagna Region)   (Italy)

Author keywords

Chloroflexi; Denaturing gradient gel electrophoresis; Marine sediment; Polychlorinated biphenyls; Reductive dechlorination

Indexed keywords

16S RRNA GENE; AROCLORS; BIOGEOCHEMICAL CONDITIONS; CHLOROFLEXI; CONTAMINATED MARINE SEDIMENTS; CONTAMINATED SEDIMENT; DECHLORINATION RATE; DENATURING GRADIENT GEL ELECTROPHORESIS; IN-SITU; MARINE SEDIMENTS; METHANE PRODUCTION; MICROBIAL COMMUNITIES; PHYLOTYPES; REDUCTIVE DECHLORINATION; SHORT-CHAIN FATTY ACIDS; SULFATE REDUCING; SULFATE REDUCTION; VANCOMYCIN; VENICE LAGOON;

ANALYTICAL GEOCHEMISTRY; ANOXIC SEDIMENTS; BACTERIA; BIOGEOCHEMISTRY; DECHLORINATION; ELECTROPHORESIS; GENES; METHANE; ORGANIC POLLUTANTS; REDUCTION; RNA; SEWAGE LAGOONS; SUBMARINE GEOLOGY; SUSPENDED SEDIMENTS;

POLYCHLORINATED BIPHENYLS;

AMPICILLIN; AROCLOR 1254; BACTERIAL RNA; DEUTERIUM; METHANE; RNA 16S; SHORT CHAIN FATTY ACID; SULFATE; VANCOMYCIN;

BIOGEOCHEMISTRY; DECHLORINATION; ELECTROKINESIS; ESTUARINE SEDIMENT; FATTY ACID; MARINE SEDIMENT; METHANE; METHANOGENIC BACTERIUM; MICROBIAL COMMUNITY; PCB; SLURRY; SULFATE; SULFATE-REDUCING BACTERIUM; SUSPENDED SEDIMENT;

ARTICLE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BIOGEOCHEMISTRY; CHLOROFLEXI; CONTROLLED STUDY; DECHLORINATION; ITALY; LAGOON; MARINE ENVIRONMENT; MICROCOSM; NONHUMAN; NUCLEOTIDE SEQUENCE; REDUCTION; RNA ANALYSIS; SEDIMENT; SIMULATION;

CHLORINE; CHLOROFLEXI; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GEOLOGIC SEDIMENTS; PHYLOGENY; POLYCHLORINATED BIPHENYLS; RNA, RIBOSOMAL, 16S; WATER POLLUTANTS, CHEMICAL;

ITALY; VENETO; VENEZIA [VENETO]; VENICE LAGOON;

BACTERIA (MICROORGANISMS); CHLOROFLEXI; CHLOROFLEXI (CLASS); TETRA; UNCULTURED BACTERIUM;

EID: 84857356859     PISSN: 03043894     EISSN: 18733336     Source Type: Journal    
DOI: 10.1016/j.jhazmat.2012.01.042     Document Type: Article

References (38)

1. Brown J.F., Wagner R.E. PCB movement, dechlorination and detoxification in the Acushnet estuarine. Environ. Toxicol. Chem. 1990, 9:1215-1233.
2. Safe S., Connor K., Ramamoorthy K., Gaido K., Maness K.S. Human exposure to endocrine-active chemicals: hazard assessment problems. Regul. Toxicol. Pharmacol. 1997, 26:52-58.
3. Carpenter D.O. Polychlorinated biphenyls (PCBs): routes of exposure and effects on human health. Rev. Environ. Health 2006, 21:1-23.
4. Stockholm Convention on Persistent Organic Pollutants, last accessed on July 2011. http://chm.pops.int/Convention/tabid/54/language/en-US/Default.aspx.
5. Bedard D.L., Quensen J.F. Microbial reductive dechlorination of polychlorinated biphenyls. Microbial Transformation and Degradation of Toxic Organic Chemicals 1995, 127-216. Wiley-Liss Division Wiley, New York. L.Y. Young, C.E. Cerniglia (Eds.).
6. Wiegel J., Wu Q. Microbial reductive dehalogenation of polychlorinated biphenyls. FEMS Microbiol. Ecol. 2000, 32:1-15.
7. Fava F., Agathos S.N. Uncertainty and research needs in the area of the biological restoration of contaminated sediments. Biological Assessment and Remediation of Contaminated Sediments. Assessment and Remediation of Contaminated Sediments, NATO Science Series, IV Earth and Environmental Sciences 2006, vol. 73:239-246. Springer, Dordrecht.
8. Bedard D.L. Polychlorinated biphenyls in aquatic sediments: environmental fate and outlook for biological treatment. Dehalogenation. Microbial Processes and Environmental Applications 2003, 443-465. Kluwer Academic Publishing Group, Dordrecht. M.M. Häggblom, I.D. Bossert (Eds.).
9. Hiraishi A. Biodiversity of dehalorespiring bacteria with special emphasis on polychlorinated biphenyl/dioxin dechlorinators. Microbes Environ. 2008, 23:1-12.
10. Cutter L.A., Watts J.E.M., Sowers K.R., May H.D. Identification of a microorganism that links its growth to the reductive dechlorination of 2,3,5,6-chlorobiphenyl. Environ. Microbiol. 2001, 3:699-709.
11. Wu Q., Watts J.E.M., Sowers K.R., May H.D. Identification of a bacterium that specifically catalyzes the reductive dechlorination of polychlorinated biphenyls with doubly flanked chlorines. Appl. Environ. Microbiol. 2002, 68:807-812.
12. Fagervold S.K., Watts J.E.M., May H.D., Sowers K.R. Sequential reductive dechlorination of meta-chlorinated polychlorinated biphenyl congeners in sediment microcosms by two different Chloroflexi phylotypes. Appl. Environ. Microbiol. 2005, 71:8085-8090.
13. Watts J.E.M., Fagervold S.K., May H.D., Sowers K.R. A PCR-based specific assay reveals a population of bacteria within the Chloroflexi associated with the reductive dehalogenation of polychlorinated biphenyls. Microbiology 2005, 181:2039-2046.
14. Yan T., LaPara T.M., Novak P.J. The reductive dechlorination of 2,3,4,5-tetrachlorobiphenyl in three different sediment cultures: evidence for the involvement of phylogenetically similar Dehalococcoides-like bacterial populations. FEMS Microbiol. Ecol. 2006, 55:248-261.
15. Yan T., LaPara T.M., Novak P.J. The effect of varying levels of sodium bicarbonate on polychlorinated biphenyl dechlorination in Hudson River sediment cultures. Environ. Microbiol. 2006, 8:1288-1298.
16. Bedard D.L., Bailey J.J., Reiss B.L., Van Slyke Jerzak G. Development and characterization of stable sediment-free anaerobic bacterial enrichment cultures that dechlorinate Aroclor 1260. Appl. Environ. Microbiol. 2006, 72:2460-2470.
17. Bedard D.L., Ritalahti K.M., Löffler F.E. The Dehalococcoides population in sediment-free mixed cultures metabolically dechlorinates the commercial polychlorinated biphenyl mixture Aroclor 1260. Appl. Environ. Microbiol. 2007, 73:2513-2521.
18. Fagervold S.K., May H.D., Sowers K.R. Microbial reductive dechlorination of Aroclor 1260 in Baltimore Harbor sediment microcosms is catalyzed by three phylotypes within the phylum Chloroflexi. Appl. Environ. Microbiol. 2007, 73:3009-3018.
19. Kjellerup B.V., Sun X., Ghosh U., May H.D., Sowers K.R. Site-specific microbial communities in three PCB-impacted sediments are associated with different in situ dechlorinating activities. Environ. Microbiol. 2008, 10:1296-1309.
20. Adrian L., Dudková V., Demnerová K., Bedard D.L. Dehalococcoides sp. strain CBDB1 extensively dechlorinates the commercial polychlorinated biphenyl mixture aroclor 1260. Appl. Environ. Microbiol. 2009, 75:4516-4524.
21. Øfjord G.D., Puhakka J.A., Ferguson J.F. Reductive dechlorination of Aroclor 1254 by marine sediment cultures. Environ. Sci. Technol. 1994, 28:2286-2294.
22. Fava F., Gentilucci S., Zanaroli G. Anaerobic biodegradation of weathered polychlorinated biphenyls (PCBs) in contaminated sediments of Porto Marghera (Venice Lagoon, Italy). Chemosphere 2003, 53:101-109.
23. Fava F., Zanaroli G., Young L.Y. Microbial reductive dechlorination of pre-existing PCBs and spiked 2,3,4,5,6-pentachlorobiphenyl in anaerobic slurries of a contaminated sediment of Venice Lagoon (Italy). FEMS Microbiol. Ecol. 2003, 44:309-318.
24. Zanaroli G., Perez-Jimenez J.R., Young L.Y., Marchetti L., Fava F. Microbial reductive dechlorination of weathered and exogenous co-planar polychlorinated biphenyls (PCBs) in an anaerobic sediment of Venice Lagoon. Biodegradation 2006, 17:19-27.
25. Zanaroli G., Balloi A., Negroni A., Daffonchio D., Young L.Y., Fava F. Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs). J. Hazard. Mater. 2010, 178:417-426.
26. Sass A.M., Sass H., Coolen M.J., Cypionka H., Overmann J. Microbial communities in the chemocline of a hypersaline deep-sea basin (Urania basin, Mediterranean Sea). Appl. Environ. Microbiol. 2001, 67:5392-5402.
27. Casamayor E.O., Massana R., Benlloch S., Ovreas L., Diez B., Goddard V.J., Gasol J.M., Joint I., Rodríguez-Valera F., Pedrós-Alió C. Changes in archaeal, bacterial and eukaryal assemblages along a salinity gradient by comparison of genetic fingerprinting methods in a multipond solar saltern. Environ. Microbiol. 2002, 4:338-348.
28. Altschul S.F., Gish W., Miller W., Myers E.W., Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 1990, 215:403-410.
29. Hendrickson E.R., Payne J.A., Young R.M., Starr M.G., Perry M.P., Fahnestock S., Ellis D.E., Ebersole R.C. Molecular analysis of Dehalococcoides 16S ribosomal DNA from chlor-oethene-contaminated sites throughout North America and Europe. Appl. Environ. Microbiol. 2002, 68:485-495.
30. Oh K-H., Ostrofsky E.B., Cho Y-C. Molecular characterization of polychlorinated biphenyl-dechlorinating populations in contaminated sediments. J. Microbiol. 2008, 46:165-173.
31. May H.D., Miller G.S., Kjellerup B.V., Sowers K.R. Dehalorespiration with polychlorinated biphenyls by an anaerobic ultramicrobacterium. Appl. Environ. Microbiol. 2008, 74:2089-2094.
32. Bossert I.D., Haggblom M.M., Young L.Y. Microbial Ecology of Dehalogenation. Dehalogenation. Microbial Processes and Environmental Applications 2003, 33-52. Kluwer Academic Publishing Group, Dordrecht. M.M. Haggblom, I.D. Bossert (Eds.).
33. Muyzer G., Smalla K. Application of denaturant gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie van Leeuwenhoek 1998, 73:127-141.
34. Fromin N., Hamelin J., Tarnawski S., Roesti D., Jourdain-Miserez K., Forestier N., Teyssier-Cuvelle S., Gillet F., Aragno M., Rossi P. Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns. Environ. Microbiol. 2002, 4:634-643.
35. Maymó-Gatell X., Chien Y., Gossett J.M., Zinder S.H. Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethane. Science 1997, 276:1568-1571.
36. Adrian L., Szewzyk U., Wecke J., Görisch H. Bacterial dehalorespiration with chlorinated benzenes. Nature 2000, 408:580-583.
37. He J., Sung Y., Krajmalnik-Brown R., Ritalahti K.M., Löffler F.E. Isolation and characterization of Dehalococcoides sp. strain FL2, a trichloroethene (TCE)- and 1,2-dichloroethene-respiring anaerobe. Environ. Microbiol. 2005, 7:1442-1450.
38. Ho C.H., Liu S.M. Effect of coplanar PCB concentration on dechlorinating microbial communities and dechlorination in estuarine sediments. Chemosphere 2011, 82:48-55.