Sequential reductive dechlorination of meta-chlorinated polychlorinated biphenyl congeners in sediment microcosms by two different Chloroflexi phylotypes

Applied and Environmental Microbiology

Volumn 71, Issue 12, 2005, Pages 8085-8090

  Fagervold, Sonja K ^a   Watts, Joy E M ^b   May, Harold D ^c   Sowers, Kevin R ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b TOWSON UNIVERSITY   (United States)

^c MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DECHLORINATION; GENES; MICROBIOLOGY; MICROORGANISMS; POLYCHLORINATED BIPHENYLS; REDUCTION; RNA;

CHLOROFLEXI PHYLOTYPES; CONGENERS; REDUCTIVE DECHLORINATION; SEDIMENT MICROCOSMS;

SEDIMENTS;

2,2',3,3',4,6' CHLOROBIPHENYL; 2,2',4,5,5' CHLOROBIPHENYL; CHLORINE; POLYCHLORINATED BIPHENYL; PRIMER DNA; RNA 16S; UNCLASSIFIED DRUG;

BACTERIUM; DECHLORINATION; PCB; POLYMERASE CHAIN REACTION;

ANAEROBIC METABOLISM; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; CHLOROFLEXI; CONTROLLED STUDY; CULTURE MEDIUM; DECHLORINATION; DEHALOCOCCOIDES; DENATURING GRADIENT GEL ELECTROPHORESIS; GENE AMPLIFICATION; MICROCOSM; MONOPHYLY; MOST PROBABLE NUMBER; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; QUALITATIVE ANALYSIS; QUANTITATIVE ASSAY; REDUCTION; SEDIMENT;

ANAEROBIOSIS; BASE SEQUENCE; BIOTRANSFORMATION; CHLORINE; CHLOROFLEXI; DNA PRIMERS; GEOLOGIC SEDIMENTS; PHYLOGENY; POLYCHLORINATED BIPHENYLS; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S;

BACTERIA (MICROORGANISMS); CHLOROFLEXI; CHLOROFLEXI (CLASS); DEHALOCOCCOIDES;

EID: 29144441528     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.12.8085-8090.2005     Document Type: Article

References (41)

1. Adrian, L., U. Szewzyk, J. Wecke, and J. Görisch. 2000. Bacterial dehalorespiration with chlorinated benzenes. Nature 408:580-583.
2. Alder, A. C., M. M. Häggblom, S. R. Oppenheimer, and L. Y. Young. 1993. Reductive dechlorination of polychlorinated biphenyls in anaerobic sediments. Environ. Sci. Technol. 27:530-538.
3. Ashley, J. T. F., and J. E. Baker. 1999. Hydrophobic organic contaminants in surficial sediments of Baltimore Harbor: inventories and sources. Environ. Toxicol. Chem. 18:838-849.
4. Bedard, D. L., and J. F. Quensen. 1995. Microbial reductive dechlorination of polychlorinated biphenyls, p. 127-216. In L. Y. Young and C. E. Cerniglia (ed.), Microbial transformation and degradation of toxic organic chemicals. John Wiley, New York, N.Y.
5. Berkaw, M., K. R. Sowers, and H. D. May. 1996. Anaerobic ortho dechlorination of polychlorinated biphenyls by estuarine sediments from Baltimore Harbor. Appl. Environ. Microbiol. 62:2534-2539.
6. Brown, J. J. F., R. E. Wagner, H. Feng, D. L. Bedard, M. J. Brennan, J. C. Carnahan, and R. J. May. 1987. Environmental dechlorination of PCBs. Environ. Toxicol. Chem. 6:579-593.
7. Bunge, M., L. Adrian, A. Kraus, M. Opel, W. G. Lorenz, J. R. Andreesen, H. Görisch, and U. Lechner. 2003. Reductive dehalogenation of chlorinated dioxins by an anaerobic bacterium. Nature 421:357-360.
8. Cochran, W. G. 1950. Estimation of bacterial densities by means of the "most probable number". Biometrics 6:105-116.
9. Cole, J. R., B. Chai, T. L. Marsh, R. J. Farris, Q. Wang, S. A. Kulam, S. Chandra, D. M. McGarrell, T. M. Schmidt, G. M. Garrity, and J. M. Tiedje. 2003. The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy. Nucleic Acids Res. 31:442-443.
10. Cupples, A. M., A. M. Spormann, and P. L. McCarty. 2003. Growth of Dehalococcoides-like microorganism on vinyl chloride and cis-dichloroethene as electron acceptors as determined by competitive PCR. Appl. Environ. Microbiol. 69:953-959.
11. Cutter, L., K. R. Sowers, and H. D. May. 1998. Microbial dechlorination of 2,3,5,6-tetrachlorobiphenyl under anaerobic conditions in the absence of soil or sediment. Appl. Environ. Microbiol. 64:2966-2969.
12. Cutter, L. A., J. E. M. Watts, K. R. Sowers, and H. D. May. 2001. Identification of a microorganism that links its growth to the reductive dechlorination of 2,3,5,6-chlorobiphenyl. Environ. Microbiol. 3:699-709.
13. Duhamel, M., K. Mo, and E. A. Edwards. 2004. Characterization of a highly enriched Dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene. Appl. Environ. Microbiol. 70:5538-5545.
14. Edwards, U., T. Rogall, H. Blocker, M. Emde, and E. C. Bottger. 1989. Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA. Nucleic Acids Res. 17:7843-7853.
15. Felsenstein, J. 1989. PHYLIP-Phytogeny Inference Package (version 3.2). Cladistics 5:164-166.
16. Fennell, D. E., I. Nijenhuis, S. F. Wilson, S. H. Zinder, and M. M. Häggblom. 2004. Dehalococcoides ethenogenes strain 195 reductively dechlorinates diverse chlorinated aromatic pollutants. Environ. Sci. Technol. 38:2075-2081.
17. Frame, G. M., J. W. Cochran, and S. S. Bøwadt. 1996. Complete PCB congener distributions for 17 Aroclor mixtures determined by 3 HRGC systems optimized for comprehensive, quantitative, congener-specific analysis. J. High Resolut. Chromatogr. 19:657-668.
18. Garton, L. S., J. S. Bonner, A. N. Ernest, and R. L. Autenrieth. 1996. Fate and transport of PCBs at the New Bedford Harbor superfund site. Environ. Toxicol. Chem. 15:736-745.
19. He, J., K. M. Ritalahti, K. L. Yang, S. S. Koenigsberg, and F. E. Löffler. 2003. Detoxification of vinyl chloride to ethene coupled to growth of an anaerobic bacterium. Nature 424:62-65.
20. Hendrickson, E. R., J. A. Payne, R. M. Young, M. G. Starr, M. P. Perry, S. Fahnestock, D. E. Ellis, and R. C. Ebersole. 2002. Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminated sites throughout North America and Europe. Appl. Environ. Microbiol. 68:485-495.
21. Iannuzzi, T. J., S. L. Huntley, N. L. Bonnevie, B. L. Finley, and R. J. Wenning. 1995. Distribution and possible sources of polychlorinated biphenyls in dated sediments from the Newark Bay estuary, New Jersey. Arch. Environ. Contam. Toxicol. 28:108-117.
22. Janse, I., J. Bok, and G. Zwart. 2004. A simple remedy against artificial double bands in denaturing gradient gel electrophoresis. J. Microbiol. Methods 57:279-281.
23. Lake, J. L., R. J. Pruell, and F. A. Osterman. 1992. An examination of dechlorination processes and pathways in New Bedford Harbor sediments. Mar. Environ. Res. 33:31-47.
24. Löffler, F. E., Q. Sun, J. Li, and J. M. Tiedje. 2002. 16S rRNA gene-based detection of tetrachloroethene-dechlorinating Desulfuromonas and Dehalcoccoides species. Appl. Environ. Microbiol. 66:1369-1374.
25. Ludwig, W., O. Strunk, R. Westram, L. Richter, H. Meier, Yadhukumar, A. Buchner, T. Lai, S. Steppi, G. Jobb, W. Förster, I. Brettske, S. Gerber, A. W. Ginhart, O. Gross, S. Grumann, S. Hermann, R. Jost, A. König, T. Liss, R. Lüßmann, M. May, B. Nonhoff, B. Reichel, R. Strehlow, A. Stamatakis, N. Stuckmann, A. Vilbig, M. Lenke, T. Ludwig, A. Bode, and K. Schleifer. 2004. ARB: a software environment for sequence data. Nucleic Acids Res. 32:1363-1371.
26. Maymo-Gatell, X., Y.-T. Chien, J. M. Gossett, and S. H. Zinder. 1997. Isolation of a bacterium that reductively dechlorinates tetrachloroethene to ethene. Science 276:1568-1571.
27. Morris, P. J., W. W. Mohn, J. F. Quensen III, J. M. Tiedje, and S. A. Boyd. 1992. Establishment of polychlorinated biphenyl-degrading enrichment culture with predominantly meta dechlorination. Appl. Environ. Microbiol. 58:3088-3094.
28. Muyzer, G., E. C. de Waal, and A. G. Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of PCR amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700.
29. Pulliam Holoman, T. R., M. A. Elberson, L. A. Cutter, H. D. May, and K. R. Sowers. 1998. Characterization of a defined 2,3,5,6-tetrachlorobiphenyl- ortho-dechlorinating microbial community by comparative sequence analysis of genes coding for 16S rRNA. Appl. Environ. Microbiol. 64:3359-3367.
30. Quensen, J. F., III, S. A. Boyd, and J. M. Tiedje. 1990. Dechlorination of four commercial polychlorinated biphenyl mixtures (Aroclors) by anaerobic microorganisms from sediments. Appl. Environ. Microbiol. 56:2360-2369.
31. Safe, S. 1992. Toxicology, structure-function relationship, and human and environmental health impacts of polychlorinated hiphenyls: progress and problems. Environ. Health Perspect. 100:259-268.
32. Saitou, N., and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
33. Stone, R. 1992. Swimming against the PCB tide. Science 255:798-799.
34. Stull, J. K., D. J. P. Swift, and A. W. Niedoroda. 1996. Contaminant dispersal on the Palos Verdes continental margin. 1. Sediments and biota near a major California wastewater discharge. Sci. Total Environ. 179:73-90.
35. Stults, J. R., O. Snoeyenbos-West, B. Methe, D. R. Lovley, and D. P. Chandler. 2001. Application of the 5′ fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments. Appl. Environ. Microhiol. 67:2781-2789.
36. Watts, J. E. M., S. K. Fagervold, G. S. Miller, C. E. Milliken, H. D. May, and K. R. Sowers. 2004. Microbial reductive dechlorination of organohalide pollutants in marine environment. Mar. Biotechnol. 6:S378-S383.
37. Watts, J. E. M., S. K. Fagervold, K. R. Sowers, and H. D. May. 2005. A PCR based specific assay reveals a population of bacteria within the Chloroflexi associated with the reductive dehalogenation of polychlorinated biphenyls. Microbiology 151:2039-2046.
38. Watts, J. E. M., Q. Wu, S. B. Schreier, H. D. May, and K. R. Sowers. 2001. Comparative analyses of PCB dechlorinating communities in enrichment cultures using three different molecular screening techniques. Environ. Microbiol. 2:710-719.
39. Wu, Q., K. R. Sowers, and H. D. May. 2000. Establishment of a polychlorinated biphenyl-dechlorinating microbial consortium, specific for doubly flanked chlorines in a defined, sediment-free medium. Appl. Environ. Microbiol. 66:49-53.
40. Wu, Q., J. E. M. Watts, K. R. Sowers, and H. D. May. 2002. Identification of a bacterium that specifically catalyzes the reductive dechlorination of polychlorinated biphenyls with doubly flanked chlorines. Appl. Environ. Microbiol. 68:807-812.
41. Wu, Q. Z., K. R. Sowers, and H. D. May. 1998. Microbial reductive dechlorination of Aroclor 1260 in anaerobic slurries of estuarine sediments. Appl. Environ. Microbiol. 64:1052-1058.