SiRNA high-throughput kinase library screen identifies protein kinase, DNA-activated catalytic polypeptide to play a role in MyD88-induced IFNA2 activation and IL-8 secretion

Biotechnology and Applied Biochemistry

Volumn 59, Issue 1, 2012, Pages 6-14

  Charlaftis, Nikolaos ^a,b   Fearon, Douglas T ^b   Schoenemeyer, Annett ^a   Morley, Peter J ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

high throughput screen; kinase; signaling; siRNA; toll like receptors

Indexed keywords

BIOLOGICAL PATHWAYS; CELLULAR GENES; HIGH-THROUGHPUT; KINASE; PROTEIN KINASE; RNA INTERFERENCE; SHORT INTERFERING RNA (SIRNA); SIRNA; TOLL-LIKE RECEPTORS;

GENES; INTERFERONS; PHYSIOLOGY; RNA; SIGNALING;

ENZYMES;

ALPHA2 INTERFERON; DNA DEPENDENT PROTEIN KINASE; INTERLEUKIN 8; MYELOID DIFFERENTIATION FACTOR 88; SMALL INTERFERING RNA;

ARTICLE; CONTROLLED STUDY; CYTOKINE PRODUCTION; CYTOKINE RELEASE; ENZYME ACTIVATION; GENE IDENTIFICATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; MOLECULAR LIBRARY; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN EXPRESSION; RNA INTERFERENCE; SIGNAL TRANSDUCTION; VALIDATION STUDY;

CELL SURVIVAL; DNA-ACTIVATED PROTEIN KINASE; GENE LIBRARY; HEK293 CELLS; HUMANS; INTERFERON-ALPHA; INTERLEUKIN-8; MYELOID DIFFERENTIATION FACTOR 88; NUCLEAR PROTEINS; PROMOTER REGIONS, GENETIC; RNA, SMALL INTERFERING; UP-REGULATION;

EID: 84857300309     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.66     Document Type: Article

References (20)

1. Kawai, T., Akira, S., (2007) Trends Mol. Med. 13, 460-469.
2. Kawai, T., Akira, S., (2007) J. Biochem. 141, 137-145.
3. Zhang, S. Y., Jouanguy, E., Sancho-Shimizu, V., von Bernuth, H., Yang, K., Abel, L., Picard, C., Puel, A., Casanova, J. L., (2007) Immunol. Rev. 220, 225-236.
4. Miller, R. L., Meng, T. C., Tomai, M. A., (2008) Drug News Perspect. 21, 69-87.
5. Krieg, A. M., Vollmer, J., (2007) Immunol. Rev. 220, 251-269.
6. Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., Mello, C. C., (1998) Nature 391, 806-811.
7. Aza-Blanc, P., Cooper, C. L., Wagner, K., Batalov, S., Deveraux, Q. L., Cooke, M. P., (2003) Mol. Cell 12, 627-637.
8. Ganesan, A. K., Ho, H., Bodemann, B., Petersen, S., Aruri, J., Koshy, S., Richardson, Z., Le, L. Q., Krasieva, T., Roth, M. G., Farmer, P., White, M. A., (2008) PLoS Genet. 4, e1000298.
9. Azorsa, D. O., Gonzales, I. M., Basu, G. D., Choudhary, A., Arora, S., Bisanz, K. M., Kiefer, J. A., Henderson, M. C., Trent, J. M., Von Hoff, D. D., Mousses, S., (2009) J. Transl. Med. 7, 43.
10. Azorsa, D. O., Robeson, R. H., Frost, D., Meec hoovet, B., Brautigam, G. R., Dickey, C., Beaudry, C., Basu, G. D., Holz, D. R., Hernandez, J. A., Bisanz, K. M., Gwinn, L., Grover, A., Rogers, J., Reiman, E. M., Hutton, M., Stephan, D. A., Mousses, S., Dunckley, T., (2010) BMC Genomics 11, 25.
11. Lwa, T. R., Tan, C. H., Lew, Q. J., Chu, K. L., Tan, J., Lee, Y. Y., Chao, S. H., (2010) J. Biotechnol. 146, 160-168.
12. Arora, S., Bisanz, K. M., Peralta, L. A., Basu, G. D., Choudhary, A., Tibes, R., Azorsa, D. O., (2010) Gynecol. Oncol., 118, 220-227.
13. Astsaturov, I., Ratushny, V., Sukhanova, A., Einarson, M. B., Bagnyukova, T., Zhou, Y., Devarajan, K., Silverman, J. S., Tikhmyanova, N., Skobeleva, N., Pecherskaya, A., Nasto, R. E., Sharma, C., Jablonski, S. A., Serebriiskii, I. G., Weiner, L. M., Golemis, E. A., (2010) Sci. Signaling 3, ra67.
14. Xiong, X., Lu, Y., Zhang, L., Wang, B., Zhao, Y., Wang, X. J., Huo, X., Shen, Y., Liang, Z., Chen, M., (2010) Comb. Chem. High Throughput Screening 13, 798-806.
15. Schaniel, C., Lee, D. F., Lemischka, I. R., (2010) Methods Enzymol. 477, 351-365.
16. Honda, K., Yanai, H., Mizutani, T., Negishi, H., Shimada, N., Suzuki, N., Ohba, Y., Takaoka, A., Yeh, W. C., Taniguchi, T., (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 15416-15421.
17. Tilesi, F., Fradiani, P., Socci, V., Willems, D., Ascenzioni, F., (2009) Curr. Opin. Mol. Ther. 11, 156-164.
18. Yotsumoto, S., Saegusa, K., Aramaki, Y., (2008) J. Immunol. 180, 809-816.
19. Ishii, K. J., Takeshita, F., Gursel, I., Gursel, M., Conover, J., Nussenzweig, A., Klinman, D. M., (2002) J. Exp. Med. 196, 269-274.
20. Hemmi, H., Kaisho, T., Takeda, K., Akira, S., (2003) J. Immunol. 170, 3059-3064.