Identification of cellular genes critical to recombinant protein production using a Gaussia luciferase-based siRNA screening system

Journal of Biotechnology

Volumn 146, Issue 4, 2010, Pages 160-168

  Lwa, Teng Rhui ^a   Tan, Chuan Hao ^a   Lew, Qiao Jing ^a   Chu, Kai Ling ^a   Tan, Janice ^a   Lee, Yih Yean ^a   Chao, Sheng Hao ^a  

^a AGENCY FOR SCIENCE   (Singapore)

Author keywords

CEBPG; CHO; EPO; HEK293; Monoclonal antibody; SiRNA

Indexed keywords

BIOLOGICAL PROCESS; BIOPHARMACEUTICAL PROTEINS; CCAAT/ENHANCER-BINDING PROTEINS; CEBPG; CELL-BASED; CELLULAR GENES; CHINESE HAMSTER OVARY CELLS; CRITICAL FACTORS; FUNCTIONAL GENOMICS; HEK-293; HIGH-THROUGHPUT; HUMAN EMBRYONIC KIDNEYS; MAMMALIAN CELLS; OVER-EXPRESSION; POTASSIUM CHANNELS; RECOMBINANT PROTEIN PRODUCTIONS; REPORTER ASSAY; SCREENING SYSTEM; SECRETED PROTEIN; TRANS-MEMBRANE PROTEINS;

GENES; MAMMALS; MONOCLONAL ANTIBODIES; POTASSIUM; RNA; SURFACE PLASMON RESONANCE;

PROTEINS;

CCAAT ENHANCER BINDING PROTEIN; CCAAT ENHANCER BINDING PROTEIN GAMMA; CELL PROTEIN; CYCLIN A1; CYCLIN A2; CYCLIN B1; CYCLIN B2; CYCLIN D1; CYCLIN D2; CYCLIN D3; CYCLIN DEPENDENT KINASE INHIBITOR 1B; CYCLIN E; ERYTHROPOIETIN; GAMMA INTERFERON; GAUSSIA LUCIFERASE; HISTONE DEACETYLASE 1; HISTONE DEACETYLASE 2; HISTONE DEACETYLASE 3; HISTONE DEACETYLASE 4; HISTONE DEACETYLASE 5; HISTONE DEACETYLASE 6; HISTONE DEACETYLASE 7; HISTONE DEACETYLASE 8; LUCIFERASE; MONOCLONAL ANTIBODY; POTASSIUM CHANNEL TETRAMERISATION DOMAIN CONTAINING 2 PROTEIN; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR HES 1; TRANSMEMBRANE PROTEIN 183A; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL STRAIN HEK293; CHO CELL; CONTROLLED STUDY; ENZYME RELEASE; FEMALE; GENE IDENTIFICATION; GENE OVEREXPRESSION; GENETIC RECOMBINATION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN SECRETION; PROTEIN SYNTHESIS; PROTEIN SYNTHESIS INHIBITION; RECOMBINANT DNA TECHNOLOGY; REPORTER GENE; UNINDEXED SEQUENCE;

ANIMALS; ANTIBODIES, MONOCLONAL; CCAAT-ENHANCER-BINDING PROTEINS; CELL GROWTH PROCESSES; CELL LINE; CHO CELLS; CRICETINAE; CRICETULUS; ERYTHROPOIETIN; ERYTHROPOIETIN, RECOMBINANT; HUMANS; INTERFERON-GAMMA; LUCIFERASES; MEMBRANE PROTEINS; POTASSIUM CHANNELS, VOLTAGE-GATED; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; RNA, SMALL INTERFERING;

CRICETULUS GRISEUS; MAMMALIA;

EID: 77950398182     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.02.016     Document Type: Article

References (17)

1. Aza-Blanc P., Cooper C.L., Wagner K., Batalov S., Deveraux Q.L., Cooke M.P. Identification of modulators of TRAIL-induced apoptosis via RNAi-based phenotypic screening. Mol. Cell 2003, 12(3):627-637.
2. Bayon Y., Trinidad A.G., de la Puerta M.L., Del Carmen R.M., Bogetz J., Rojas A., De Pereda J.M., Rahmouni S., Williams S., Matsuzawa S., et al. KCTD5, a putative substrate adaptor for cullin3 ubiquitin ligases. FEBS J. 2008, 275(15):3900-3910.
3. Chao S.H., Harada J.N., Hyndman F., Gao X., Nelson C.G., Chanda S.K., Caldwell J.S. PDX1, a cellular homeoprotein, binds to and regulates the activity of human cytomegalovirus immediate early promoter. J. Biol. Chem. 2004, 279(16):16111-16120.
4. Chusainow J., Yang Y.S., Yeo J.H., Toh P.C., Asvadi P., Wong N.S., Yap M.G. A study of monoclonal antibody-producing CHO cell lines: what makes a stable high producer?. Biotechnol. Bioeng. 2009, 102(4):1182-1196.
5. Crawford E.L., Blomquist T., Mullins D.N., Yoon Y., Hernandez D.R., Al Bagdhadi M., Ruiz J., Hammersley J., Willey J.C. CEBPG regulates ERCC5/XPG expression in human bronchial epithelial cells and this regulation is modified by E2F1/YY1 interactions. Carcinogenesis 2007, 28(12):2552-2559.
6. Davidsson J., Andersson A., Paulsson K., Heidenblad M., Isaksson M., Borg A., HeldrupJ, Behrendtz M., Panagopoulos I., Fioretos T., et al. Tiling resolution array comparative genomic hybridization, expression and methylation analyses of dup(1q) in Burkitt lymphomas and pediatric high hyperdiploid acute lymphoblastic leukemias reveal clustered near-centromeric breakpoints and overexpression of genes in 1q22-32.3. Hum. Mol. Genet. 2007, 16(18):2215-2225.
7. Huang Q., Raya A., DeJesus P., Chao S.H., Quon K.C., Caldwell J.S., Chanda S.K., Izpisua-Belmonte J.C., Schultz P.G. Identification of p53 regulators by genome-wide functional analysis. Proc. Natl. Acad. Sci. U.S.A. 2004, 101(10):3456-3461.
8. Iwakoshi N.N., Lee A.H., Vallabhajosyula P., Otipoby K.L., Rajewsky K., Glimcher L.H. Plasma cell differentiation and the unfolded protein response intersect at the transcription factor XBP-1. Nat. Immunol. 2003, 4(4):321-329.
9. Ku S.C., Ng D.T., Yap M.G., Chao S.H. Effects of overexpression of X-box binding protein 1 on recombinant protein production in Chinese hamster ovary and NS0 myeloma cells. Biotechnol. Bioeng. 2008, 99(1):155-164.
10. Lee Y.Y., Wong K.T., Nissom P.M., Wong D.C., Yap M.G. Transcriptional profiling of batch and fed-batch protein-free 293-HEK cultures. Metab. Eng. 2007, 9(1):52-67.
11. Mullins D.N., Crawford E.L., Khuder S.A., Hernandez D.A., Yoon Y., Willey J.C. CEBPG transcription factor correlates with antioxidant and DNA repair genes in normal bronchial epithelial cells but not in individuals with bronchogenic carcinoma. BMC Cancer 2005, 5:141.
12. Nerlov C. The C/EBP family of transcription factors: a paradigm for interaction between gene expression and proliferation control. Trends Cell Biol. 2007, 17(7):318-324.
13. Tannous B.A., Kim D.E., Fernandez J.L., Weissleder R., Breakefield X.O. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Mol. Ther. 2005, 11(3):435-443.
14. Tigges M., Fussenegger M. Xbp1-based engineering of secretory capacity enhances the productivity of Chinese hamster ovary cells. Metab. Eng. 2006, 8:264-272.
15. Wang Y., Zheng Y., Luo F., Fan X., Chen J., Zhang C., Hui R. KCTD10 interacts with proliferating cell nuclear antigen and its down-regulation could inhibit cell proliferation. J. Cell. Biochem. 2009, 106(3):409-413.
16. Wedel A., Ziegler-Heitbrock H.W. The C/EBP family of transcription factors. Immunobiology 1995, 193(2-4):171-185.
17. Yu H., Jiang H., Zhou Q., Yang J., Cun Y., Su B., Xiao C., Wang W. Origination and evolution of a human-specific transmembrane protein gene, c1orf37-dup. Hum. Mol. Genet. 2006, 15(11):1870-1875.