Cell analysis in cerebrospinal fluid (CSF) using Sysmex ® hematology analyzers XT-4000i and XE-5000: Evaluation with CSF controls of the Joint German Society for Clinical Chemistry and Laboratory Medicine (DGKL)

Cytometry Part A

Volumn 81 A, Issue 3, 2012, Pages 255-264

  Kleine, Tilmann O ^a   Nebe, Carl Thomas ^b   Löwer, Christa ^a   Geilenkeuser, Wolf Jochen ^c   Dorn Beineke, Alexandra ^d  

^a UNIVERSITY HOSPITAL MARBURG   (Germany)

^b Im Institut für Immunologie und Genetik   (Germany)

^c Referenzinstitut für Bioanalytik   (Germany)

^d DR HORST SCHMIDT KLINIKEN   (Germany)

Author keywords

Cerebrospinal fluid cell analysis; Chamber cell counting; Flow cytometry; Hematology analyzers Sysmex XT 4000i, XE 5000

Indexed keywords

CD14 ANTIGEN; CD45 ANTIGEN; FC RECEPTOR; MONOCLONAL ANTIBODY;

ARTICLE; CEREBROSPINAL FLUID; CYTOLOGY; ERYTHROCYTE; ERYTHROCYTE COUNT; FLOW CYTOMETRY; GRANULOCYTE; HEMATOLOGY; HUMAN; IMMUNOLOGY; LEUKOCYTE COUNT; METHODOLOGY; MONONUCLEAR CELL; NEUTROPHIL; STAINING;

ANTIBODIES, MONOCLONAL; ANTIGENS, CD14; ANTIGENS, CD45; CEREBROSPINAL FLUID; ERYTHROCYTE COUNT; ERYTHROCYTES; FLOW CYTOMETRY; GRANULOCYTES; HEMATOLOGY; HUMANS; LEUKOCYTE COUNT; LEUKOCYTES, MONONUCLEAR; NEUTROPHILS; RECEPTORS, IGG; STAINING AND LABELING;

EID: 84857263201     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.22014     Document Type: Article

References (19)

1. Kleine TO, Nebe CT, Löwer C, Lehmitz R, Geilenkeuser WJ, Kruse R, Dorn-Beineke A. Evaluation of cell counting and leukocyte differentiating in cerebrospinal fluid controls using hematology analyzers by the German society for clinical chemistry and laboratory medicine. Clin Chem Lab Med 2010; 48: 839-848.
2. Kleine TO. Mechanisierte Zählung und Differenzierung von Liquorzellen. Lab Med 1991; 15: 51-59.
3. Macey MG, McCarthy DA. Quantitation of adhesion molecules and other function-associated antigens on human peripheral blood leukocytes. Cytometry 1993; 14: 898-908.
4. Kleine TO, Albrecht J. Vereinfachte Durchflußzytometrie von Liquorzellen mit FACSan. Lab Med 1991; 15: 73-78.
5. Kleine TO, Nebe CT, Löwer C, Lehmitz R, Kruse R, Geilenkeuser WJ, Dorn-Beineke A. Modifications of hematology analyzers to improve cell counting and leukocyte differentiating in cerebrospinal fluid controls of the joint German society for clinical chemistry and laboratory medicine. Cytometry Part A 2009; 75A: 688-691.
6. Passing H, Bablok W. A new biometrical procedure for testing the equality of measurements from two different analytical methods, Part I. J Clin Chem Clin Biochem 1983; 21: 709-720.
7. Passing H, Bablok W. Comparison of several regression procedures for method comparison studies and determination of sample sizes. Application of linear regression procedures for method comparison studies in Clinical Chemistry, Part II. J Clin Chem Clin Biochem 1984; 22: 431-445.
8. McCarthy DA, Macey MG, Cahill MR, Newland AC. Effect of fixation on quantification of the expression of leukocyte function-associated surface antigens. Cytometry 1994; 17: 39-49.
9. Macey MG, McCarthy DA, Davies C, Newland AC. The Q-prep system: Effect on the apparent expression of leukocyte cell surface antigens. Cytometry 1997; 30: 67-71.
10. Campbell AR, Andress DL, Swenson ER. Identification and characterization of human neutrophil carbonic anhydrase. J Leukocyte Biol 1994; 55: 343-348.
11. Demir Y, Demir N, Alayli A. Cytosol and noncytosolic carbonic anhydrase enzymes from bovine leukocytes. Prep Biochem Biotechnol 2001; 31: 421-431.
12. Kleine TO. Liquor-Zellreaktionen; Liquor-Zellzählung. In: Gressner AM, Arndt T, editors. Lexikon der Medizinischen Laboratoriumsdiagnostik, 1st ed. Heidelberg: Springer Medizin Verlag; 2007, pp 845-850.
13. Kleine T, editor. Neue Labormethoden für die Liquordiagnostik. Stuttgart: G. Thieme Verlag; 1980.
14. Boer K, Deufel T, Reinhoefer M. Evaluation of the XE-5000 for the automated analysis of blood cells in cerebrospinal fluid. Clin Biochem 2009; 42: 684-691.
15. De Jonge R, Brouwer R, de Graaf, MT, Luitwieler RL, Fleming C, de Frankrijker-Merkestijn M, Sillevis Smitt PAE, Boonstra JG, Lindemanns J. Evaluation of the new body fluid mode on the Sysmex XE-5000 for counting leukocytes and erythrocytes in cerebrospinal fluid and other body fluids. Clin Chem Lab Med 2010; 48: 665-675.
16. Paris A, Nhan T, Cornet E, Perol JP, Malet M, Troussard X. Performance evaluation of the body fluid mode on the platform Sysmex-5000 series automated hematology analyzer. Int J Lab Hem 2010; 32: 539-547.
17. Sandhus LM, Ciarlini P, Kidric D, Dillman C, O'Riordan MA. Automated cerebrospinal fluid cell counts using the Sysmexy XE-5000. Is it time for new reference ranges? Am J Clin Pathol 2010; 134: 734-738.
18. Macey MG, McCarthy DA, Milne T, Cavenagh JD, Newland AC. Comparative study of five commercial reagents for preparing normal and leukemic lymphocytes for immunophenotypic analysis by flow cytometry. Cytometry 1999; 38: 153-160.
19. McCarthy DA, Macey MG. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods 1993; 163: 155-160.