Probing multimodal ligand binding regions on ubiquitin using nuclear magnetic resonance, chromatography, and molecular dynamics simulations

Journal of Chromatography A

Volumn 1229, Issue , 2012, Pages 113-120

  Holstein, Melissa A ^a   Chung, Wai Keen ^a   Parimal, Siddharth ^a   Freed, Alexander S ^a   Barquera, Blanca ^a   McCallum, Scott A ^a   Cramer, Steven M ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Chemical shift perturbation mapping; Molecular dynamics simulations; Multimodal chromatography; Nuclear magnetic resonance; Site directed mutagenesis

Indexed keywords

BINDING INTERACTION; CATION EXCHANGE RESINS; CHEMICAL SHIFT PERTURBATION MAPPING; CHROMATOGRAPHIC RETENTION; CHROMATOGRAPHIC SYSTEMS; HIGH AFFINITY; LIGAND AFFINITY; LIGAND BINDING; MD SIMULATION; MOLECULAR DYNAMICS SIMULATIONS; MOLECULAR LEVELS; MULTI-MODAL; MUTANT PROTEINS; NMR CHEMICAL SHIFT PERTURBATIONS; NUCLEAR MAGNETIC RESONANCE CHEMICAL SHIFTS; PROTEIN-LIGAND INTERACTIONS; SITE DIRECTED MUTAGENESIS; SYNERGISTIC INTERACTION; UBIQUITIN; WILD TYPES; WILD-TYPE PROTEINS;

BINDING ENERGY; CHEMICAL SHIFT; EXPERIMENTS; LIGANDS; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE; PROTEINS;

CHROMATOGRAPHY;

UBIQUITIN;

ARTICLE; BINDING AFFINITY; BINDING SITE; CATION EXCHANGE; CHROMATOGRAPHY; CONTROLLED STUDY; LIGAND BINDING; MOLECULAR DYNAMICS; MOLECULAR MODEL; MOLECULAR PROBE; MUTATIONAL ANALYSIS; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; WILD TYPE;

BINDING SITES; HUMANS; LIGANDS; MOLECULAR DYNAMICS SIMULATION; MUTAGENESIS, SITE-DIRECTED; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN BINDING; UBIQUITIN;

EID: 84857140420     PISSN: 00219673     EISSN: 18733778     Source Type: Journal    
DOI: 10.1016/j.chroma.2011.12.101     Document Type: Article

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