NMR chemical shift mapping of the binding site of a protein proteinase inhibitor: Changes in the 1H, 13C and 15N NMR chemical shifts of turkey ovomucoid third domain upon binding to bovine chymotrypsin Aα

Journal of Molecular Recognition

Volumn 14, Issue 3, 2001, Pages 166-171

  Song, Jikui ^a   Markley, John L ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Binding site mapping; Chymotrypsin A ; NMR; OMTKY3

Indexed keywords

AMINO ACID; CARBON 13; CHEMICAL STRUCTURE; CHYMOTRYPSIN; CONSENSUS SEQUENCE; MOLECULAR RECOGNITION; NITROGEN 15; NUCLEAR MAGNETIC RESONANCE IMAGING; NUCLEOTIDE BINDING SITE; PROTEIN BINDING; PROTEIN DOMAIN; PROTEINASE INHIBITOR; SERINE PROTEINASE; TURKEY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CATTLE; CHYMOTRYPSIN; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; OVOMUCIN; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; TRYPSIN INHIBITORS; TURKEYS;

AVES; BOVINAE;

EID: 0034950683     PISSN: 09523499     EISSN: None     Source Type: Journal    
DOI: 10.1002/jmr.530     Document Type: Article

References (25)

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3. 15N relaxation analysis of the C-terminal KH domain of heterogeneous nuclear ribonucleoprotein K
4. Soybean trypsin inhibitor (Kunitz) and its complex with trypsin. Carbon-13 nuclear magnetic resonance studies of the reactive site arginine
5. 15N signals of turkey ovomucoid third domain at pH 2.0
6. Refined crystal structure of the molecular complex of Streptomyces griseus proteinase B, a serine proteinase, with the third domain of the ovomucoid inhibitor from turkey
7. Crystal and molecular structures of the complex of α-chymotrypsin with its inhibitor turkey ovomucoid third domain at 1.8 Å resolution
8. 15N spectra of larger proteins, heteronuclear triple-resonance 3-dimensional spectroscopy: Application to calmodulin
9. Overexpression and purification of avian ovomucoid third domains in Escherichia coli
10. Structure of the complex formed by bovine trypsin and bovine pancreatictrypsin inhibitor. II. Crystallographic refinement at 1.9 Å resolution
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12. a by spectrofluorimetric titration
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17. Structure and interaction site of the regulatory domain of troponin-C when complexed with the 96-148 region of troponin-I
18. 1 residues in serine proteinase inhibitors undergo large pK shifts on complex formation
19. Thermodynamic criterion for the conformation of P1 residues of substrates and of inhibitors in complexes with serine proteinases
20. Carbon-13 nuclear magnetic resonance studies of the selectively isotope-labeled reactive site peptide bond of the basic pancreatic trypsin inhibitor in the complexes with trypsin, trypsinogen, and anhydrotrypsin
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23. 2H labeled proteins with high sensitivity
24. 2H-labeled proteins: Application to a 37-kDa Trp repressor DNA complex