High cell-density expression system: A novel method for extracellular production of difficult-to-express proteins

Journal of Bioscience and Bioengineering

Volumn 113, Issue 2, 2012, Pages 154-159

  Kimata, Koichi ^a   Yamaguchi, Masaya ^a   Saito, Yuta ^a   Hata, Hiroki ^a   Miyake, Kasumi ^a   Yamane, Tsuneo ^b   Nakagawa, Yuko ^c,d   Yano, Akira ^c   Ito, Keisuke ^a   Kawarasaki, Yasuaki ^a  

^a UNIVERSITY OF SHIZUOKA   (Japan)

^b CHUBU UNIVERSITY   (Japan)

^c IWATE BIOTECHNOLOGY RESEARCH CENTER   (Japan)

^d ICHINOSEKI NATIONAL COLLEGE OF TECHNOLOGY   (Japan)

Author keywords

Difficult to express protein; Extracellular expression; High cell density expression; Lentinula edodes laccase; Saccharomyces cerevisiae

Indexed keywords

ALTERNATIVE APPROACH; ASPERGILLUS ORYZAE; COST-EFFICIENT; EXPRESSION SYSTEM; EXTRACELLULAR; HIGH CELL DENSITY CULTURES; HIGH CELL-DENSITY EXPRESSION; HIGHER YIELD; HOST CELLS; LACCASES; LENTINULA EDODES; PRODUCTION SYSTEM; RECOMBINANT PROTEIN; RECOMBINANT YEAST CELLS; RESTING CELLS; STATIONARY PHASE; YEAST STRAIN;

ASPERGILLUS; CELLS; ESCHERICHIA COLI; PROTEINS; YEAST;

CELL CULTURE;

LENTINULA EDODES EXTRACT;

AERATION; ARTICLE; ASPERGILLUS ORYZAE; CELL CULTURE; CELL DENSITY; CELL PROLIFERATION; CONTROLLED STUDY; ENZYME SYNTHESIS; ESCHERICHIA COLI; FUNGAL STRAIN; FUNGUS GROWTH; GENE EXPRESSION; LENTINULA EDODES; MICROBIAL GROWTH; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; TURBIDITY; YEAST CELL;

ASPERGILLUS ORYZAE; BETA-GALACTOSIDASE; CELL COUNT; CELL CULTURE TECHNIQUES; ESCHERICHIA COLI; LACCASE; PLASMIDS; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SHIITAKE MUSHROOMS;

ASPERGILLUS ORYZAE; ESCHERICHIA COLI; LENTINULA EDODES; SACCHAROMYCES CEREVISIAE;

EID: 84856807407     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.10.007     Document Type: Article

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