Live-Cell Assays to Identify Regulators of ER-to-Golgi Trafficking

Traffic

Volumn 13, Issue 3, 2012, Pages 416-432

  Lisauskas, Tautvydas ^a   Matula, Petr ^a,b   Claas, Christoph ^a   Reusing, Susanne ^a   Wiemann, Stefan ^c   Erfle, Holger ^a   Lehmann, Lars ^a   Fischer, Peter ^a   Eils, Roland ^a   Rohr, Karl ^a   Storrie, Brian ^d   Starkuviene, Vytaute ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b MASARYK UNIVERSITY   (Czech Republic)

^c GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

^d UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

BFA; ER to Golgi trafficking; GalT; GOT1B; SACM1L; USE1; YIPF

Indexed keywords

BREFELDIN A; GALACTOSYLTRANSFERASE; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL ASSAY; CELL FUNCTION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; FLUORESCENCE MICROSCOPY; GENE OVEREXPRESSION; GOLGI COMPLEX; HOMEOSTASIS; INTRACELLULAR TRANSPORT; MICROSCOPY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; RAT; RNA INTERFERENCE;

ANIMALS; BIOLOGICAL ASSAY; CELLS, CULTURED; ENDOPLASMIC RETICULUM; GOLGI APPARATUS; KIDNEY; MICROSCOPY, FLUORESCENCE; PROTEIN TRANSPORT; RATS;

EID: 84856772585     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2011.01318.x     Document Type: Article

References (115)

1. Wilson C, Venditti R, Rega LR, Colanzi A, D'Angelo G, De Matteis MA. The Golgi apparatus: an organelle with multiple complex functions. Biochem J 2010;433:1-9.
2. Sütterlin C, Colanzi A. The Golgi and the centrosome: building a functional partnership. J Cell Biol 2010;188:621-628. 10.1083/jcb.200910001.
3. Dennis JW, Nabi IR, Demetriou M. Metabolism, cell surface organization, and disease. Cell 2009;139:1229-1241. 10.1016/j.cell.2009.12.008.
4. Kondylis V, Rabouille C. The Golgi apparatus: lessons from Drosophila. FEBS Lett 2009;583:3827-3838. 10.1016/j.febslet.2009.09.048.
5. Pulvirenti T, Giannotta M, Capestrano M, Capitani M, Pisanu A, Polishchuk RS, San Pietro E, Beznoussenko GV, Mironov AA, Turacchio G, Hsu VW, Sallese M, Luini A. A traffic-activated Golgi-based signalling circuit coordinates the secretory pathway. Nat Cell Biol 2008;10:912-922. 10.1038/ncb1751.
6. Hicks SW, Machamer CE. Golgi structure in stress sensing and apoptosis. Biochimica et Biophysica Acta 2005;1744:406-414. 10.1016/j.bbamcr.2005.03.002.
7. Marsh BJ, Howell KE. The mammalian Golgi-complex debates. Nat Rev Mol Cell Biol 2002;3:789-795. 10.1038/nrm933.
8. Perinetti G, Müller T, Spaar A, Polishchuk R, Luini A, Egner A. Correlation of 4Pi and electron microscopy to study transport through single Golgi stacks in living cells with super resolution. Traffic 2009;10:379-391. 10.1111/j.2009.00875.x.
9. Bouchet-Marquis C, Starkuviene V, Grabenbauer M. Golgi apparatus studied in vitreous sections. J Microscopy 2008;230:308-316. doi:10.1111/j1365-28182008.01988.x.
10. Koga D, Ushiki T. Three-dimensional ultrastructure of the Golgi apparatus in different cells: high-resolution scanning electron microscopy of osmium-macerated tissues. Arch Histol Cytol 2006;69:357-374. 10.1679/aohc.69.357.
11. Grabenbauer M, Geerts WJC, Fernadez-Rodriguez J, Hoenger A, Koster AJ, Nilsson T. Correlative microscopy and electron tomography of GFP through photooxidation. Nat Methods 2005;2:857-862. 10.1038/nmeth806.
12. Marsh BJ, Volkmann N, McIntosh JR, Howell KE. Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells. Proc Natl Acad Sci U S A 2004;101:5565-5570. 10.1073/pnas.0401242101.
13. Egner A, Verrier S, Goroshkov A, Söling H-D, Hell SW. 4Pi-microscopy of the Golgi apparatus in live mammalian cells. J Struct Biol 2004;147:70-76. 10.1016/j.jsb.2003.10.006.
14. Marsh BJ, Mastronarde DN, Buttle KF, Howell KE, McIntosh JR. Organellar relationships in the Golgi region of the pancreatic beta cell line, HIT-T15, visualized by high resolution electron tomography. Proc Natl Acad Sci U S A 2001;98:2399-2406. 10.1073/pnas.051631998.
15. Ladinsky MS, Mastronarde DN, McIntosh JR, Howell KE, Staehelin LA. Golgi structure in three dimensions: functional insights from the normal rat kidney cell. J Cell Biol 1999;144:1135-1149. 10.1083/jcb.144.6.1135.
16. Suzaki E, Kataoka K. Three-dimensional visualization of the Golgi apparatus: observation of Brunner's gland cells by a confocal laser scanning microscope. JStruct Biol 1999;128:131-138. 10.1006/jsbi.1999.4175.
17. Rambourg A, Clermont Y. Three-dimensional electron microscopy: structure of the Golgi apparatus. Eur J Cell Biol 1990;51:189-200.
18. Beams HW, Kessel RG. The Golgi apparatus: structure and function. Int Rev Cytol 1968;23:209-276.
19. Mollenhayer HH, Whaley WG. An observation on the functioning of the Golgi apparatus. J Cell Biol 1963;17:222-225. 10.1083/jcb.17.1.222.
20. De Matteis MA, Luini A. Exiting the Golgi complex. Nat Rev Mol Cell Biol 2008;9:273-284. 10.1038/nrm2378.
21. Storrie B. Maintenance of Golgi apparatus structure in the face of continuous protein recycling to the endoplasmic reticulum: making ends meet. Int Rev Cytol 2005;244:69-94. 10.1016/S0074-7696.(05)
22. Lee MCS, Miller EA, Goldberg J, Orci L, Schekman R. Bi-directional protein transport between the ER and Golgi. Ann Rev Cell Dev Biol 2004;20:87-123. 10.1146/annurev.cellbio.20.010403.105307.
23. Kondylis V, Tang Y, Fuchs F, Boutros M, Rabouille C. Identification of ER proteins involved in the functional organisation of the early secretory pathway in Drosophila cells by a targeted RNAi screen. PLoS One 2011;6:e17173. 10.1371/journal.pone.0017173.
24. Wendler F, Gillingham AK, Sinka R, Rosa-Ferreira C, Gordon DE, Franch-Marro X, Peden AA, Vincent J-P, Munro S. A genome-wide RNA interference screen identifies two novel components of the metazoan secretory pathway. EMBO J 2010;29:304-314. 10.1038/emboj.2009.350.
25. Boulaflous A, Faso C, Brandizzi F. Deciphering the Golgi apparatus: from imaging to genes. Traffic 2008;9:1613-1617. 10.1111/j.2008.00769.x.
26. Bard F, Casano L, Mallabiabarrena A, Wallace E, Saito K, Kitayama H, Guizzunti G, Hu Y, Wendler F, Dasgupta R, Perrimon N, Malhotra V. Functional genomics reveals genes involved in protein secretion and Golgi organization. Nature 2006;439:604-607. 10.1038/nature04377.
27. Gilchrist A, Au CE, Hiding J, Bell AW, Fernandez-Rodriguez J, Lesimple S, Nagaya H, Roy L, Gosline SJC, Hallett M, Paiement J, Kearney RE, Nilsson T, Bergeron JJM. Quantitative proteomics analysis of the secretory pathway. Cell 2006;127:1265-1281. 10.1016/j.cell.2006.10.036.
28. Storrie B, White J, Röttger S, Stelzer EH, Suganuma T, Nilsson T. Recycling of Golgi-resident glycosyltransferases through the ER reveals a novel pathway and provides an explanation for nocodazole-induced Golgi scattering. J Cell Biol 1998;143:1505-1521. 10.1083/jcb.143.6.1505.
29. Cole NB, Sciaky N, Marotta A, Song J, Lippincott-Schwartz J. Golgi dispersal during microtubule disruption: regeneration of Golgi stacks at peripheral endoplasmic reticulum exit sites. Mol Biol Cell 1996;7:631-650.
30. Jiang S, Rhee SW, Gleeson PA, Storrie B. Capacity of the Golgi apparatus for cargo transport prior to complete assembly. Mol Biol Cell 2006;17:4105-4117. 10.1091/mbc.E05-12-1112.
31. Puri S, Linstedt AD. Capacity of the Golgi apparatus for biogenesis from the endoplasmic reticulum. Mol Biol Cell 2003;14:5011-5018. 10.1091/mbc.E03-06-0437.
32. Aridor M, Balch WE. Kinase signaling initiates coat complex II (COPII) recruitment and export from the mammalian endoplasmic reticulum. J Biol Chem 2000;275:35673-35676. 10.1074/jbc.C000449200.
33. Figueiredo P, Brown WJ. Clofibrate inhibits membrane trafficking to the Golgi complex and induces its retrograde movement to the endoplasmic reticulum. Cell Biol Toxicol 1999;15:311-323.
34. Sáenz JB, Sun WJ, Chang JW, Li J, Bursulaya B, Gray NS, Haslam DB. Golgicide A reveals essential roles for GBF1 in Golgi assembly and function. Nat Chem Biol 2009;5:157-165. 10.1038/nchembio.144.
35. Boal F, Guetzoyan L, Sessions RB, Zeghouf M, Spooner RA, Lord JM, Cherfils J, Clarkson GJ, Roberts LM, Stephens DJ. LG186: an inhibitor of GBF1 function that causes Golgi disassembly in human and canine cells. Traffic 2010;11:1537-1551. 10.1111/j.2010.01122.x.
36. Schaub BE, Berger EG, Rohrer J. Dissection of a novel molecular determinant mediating Golgi to trans-Golgi network transition. Cell Mol Life Sci 2008;65:3677-3687. 10.1007/s00018-008-8446-y.
37. Manolea F, Claude A, Chun J, Rosas J, Melançon P. Distinct functions for Arf guanine nucleotide exchange factors at the Golgi complex: GBF1 and BIGs are required for assembly and maintenance of the Golgi stack and trans-Golgi network, respectively. Mol Biol Cell 2008;19:523-535. 10.1091/mbc.E07-04-0394.
38. Borsig L, Imbach T, Höchli M, Berger EG. alpha1, 3Fucosyltransferase VI is expressed in HepG2 cells and codistributed with beta1, 4galactosyltransferase I in the Golgi apparatus and monensin-induced swollen vesicles. Glycobiology 1999;9:1273-1280.
39. Pan H, Yu J, Zhang L, Carpenter A, Zhu H, Li L, Ma D, Yuan J. A novel small molecule regulator of guanine nucleotide exchange activity of the ADP-ribosylation factor and Golgi membrane trafficking. J Biol Chem 2008;283:31087-31096. 10.1074/jbc.M806592200.
40. Drakakaki G, Robert S, Raikhel NV, Hicks GR. Chemical dissection of endosomal pathways. Plant Signaling Behav 2009;4:57-62.
41. Dinter A, Berger EG. Golgi-disturbing agents. Histochem Cell Biol 1998;109:571-590. 10.1007/s004180050256.
42. Jackson CL. Brefeldin A revealing the fundamental principles governing membrane dynamics and protein transport. Subcell Biochem 2000;34:233-272.
43. Klausner RD, Donaldson JG, Lippincott-Schwartz J. Brefeldin A: insights into the control of membrane traffic and organelle structure. J Cell Biol 1992;116:1071-1080.
44. Mossessova E, Corpina RA, Goldberg J. Crystal structure of ARF1*Sec7 complexed with brefeldin A and its implications for the guanine nucleotide exchange mechanism. Mol Cell 2003;12:1403-1411. 10.1016/S1097-2765.(03)
45. Renault L, Guibert B, Cherfils J. Structural snapshots of the mechanism and inhibition of a guanine nucleotide exchange factor. Nature 2003;426:525-530. 10.1038/nature02197.
46. Peyroche A, Antonny B, Robineau S, Acker J, Cherfils J, Jackson CL. Brefeldin A acts to stabilize an abortive ARF-GDP-Sec7 domain protein complex: involvement of specific residues of the Sec7 domain. Mol Cell 1999;3:275-285. 10.1016/S1097-2765.(00)
47. Dascher C, Balch WE. Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus. J Biol Chem 1994;269:1437-1448.
48. Lippincott-Schwartz J, Yuan LC, Bonifacino JS, Klausner RD. Rapid redistribution of Golgi proteins into the ER in cells treated with brefeldin A: evidence for membrane cycling from Golgi to ER. Cell 1989;56:801-813. 10.1016/0092-8674.(89)
49. Fujiwara T, Oda K, Yokota S, Takatsuki A, Ikehara Y. Brefeldin A causes disassembly of the Golgi complex and accumulation of secretory proteins in the endoplasmic reticulum. J Biol Chem 1988;263:18545-18552.
50. Cherfils J, Melançon P. On the action of brefeldin A on Sec7-stimulated membrane-recruitment and GDP/GTP exchange of Arf proteins. Biochem Soc Trans 2005;33:635-638. 10.1042/BST0330635.
51. Niu T-K, Pfeifer AC, Lippincott-Schwartz J, Jackson CL. Dynamics of GBF1, a brefeldin A-sensitive Arf1 exchange factor at the Golgi. Mol Biol Cell 2005;16:1213-1222. 10.1091/mbc.E04-07-0599.
52. Padilla PI, Pacheco-Rodriguez G, Moss J, Vaughan M. Nuclear localization and molecular partners of BIG1, a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP-ribosylation factors. Proc Natl Acad Sci U S A 2004;101:2752-2757. 10.1073/pnas.0307345101.
53. Claude A, Zhao BP, Kuziemsky CE, Dahan S, Berger SJ, Yan JP, Armold AD, Sullivan EM, Melançon P. GBF1: a novel Golgi-associated BFA-resistant guanine nucleotide exchange factor that displays specificity for ADP-ribosylation factor 5. J Cell Biol 1999;146:71-84. 10.1083/jcb.146.1.71.
54. Torii S, Banno T, Watanabe T, Ikehara Y, Murakami K, Nakayama K. Cytotoxicity of brefeldin A correlates with its inhibitory effect on membrane binding of COP coat proteins. J Biol Chem 1995;270:11574-11580.
55. Bannykh SI, Plutner H, Matteson J, Balch WE. The role of ARF1 and rab GTPases in polarization of the Golgi stack. Traffic 2005;6:803-819. 10.1111/j.2005.00319.x.
56. Ho HH, He CY, Graffenried CL, Murrells LJ, Warren G. Ordered assembly of the duplicating Golgi in Trypanosoma brucei. Proc Natl Acad Sci U SA 2006;103:7676-7681. 10.1073/pnas.0602595103.
57. Altan-Bonnet N, Sougrat R, Liu W, Snapp EL, Ward T, Lippincott-Schwartz J. Golgi inheritance in mammalian cells is mediated through endoplasmic reticulum export activities. Mol Biol Cell 2006;17:990-1005. 10.1091/mbc.E05-02-0155.
58. Ward TH, Polishchuk RS, Caplan S, Hirschberg K, Lippincott-Schwartz J. Maintenance of Golgi structure and function depends on the integrity of ER export. J Cell Biol 2001;155:557-570. 10.1083/jcb.200107045.
59. Sciaky N, Presley J, Smith C, Zaal KJ, Cole N, Moreira JE, Terasaki M, Siggia E, Lippincott-Schwartz J. Golgi tubule traffic and the effects of brefeldin A visualized in living cells. J Cell Biol 1997;139:1137-1155. 10.1083/jcb.139.5.1137.
60. Nelson KK, Holmer M, Lemmon SK. SCD5, a suppressor of clathrin deficiency, encodes a novel protein with a late secretory function in yeast. Mol Biol Cell 1996;7:245-260.
61. García-Mata R, Szul T, Alvarez C, Sztul E. ADP-ribosylation factor/COPI-dependent events at the endoplasmic reticulum-Golgi interface are regulated by the guanine nucleotide exchange factor GBF1. Mol Biol Cell 2003;14:2250-2261. 10.1091/mbc.E02-11-0730.
62. Kawamoto K, Yoshida Y, Tamaki H, Torii S, Shinotsuka C, Yamashina S, Nakayama K. GBF1, a guanine nucleotide exchange factor for ADP-ribosylation factors, is localized to the cis-Golgi and involved in membrane association of the COPI coat. Traffic 2002;3:483-495. 10.1034/j.2002.30705.x.
63. Szul T, Grabski R, Lyons S, Morohashi Y, Shestopal S, Lowe M, Sztul E. Dissecting the role of the ARF guanine nucleotide exchange factor GBF1 in Golgi biogenesis and protein trafficking. J Cell Sci 2007;120:3929-3940. 10.1242/jcs.010769.
64. Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S. Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing. EMBO Rep 2000;1:287-292. 10.1093/embo-reports/kvd058.
65. Duden R, Grifiths G, Frank R, Argos P, Kreis TE. Beta-COP, a 110 kD protein associated with non-clathrin coated vesicles and the Golgi complex, shows homology to beta-adaptin. Cell 1991;64:649-665.
66. Donaldson JG, Lippincott-Schwartz J, Bloom GS, Kreis TE, Klausner RD. Dissociation of a 110-kD peripheral membrane protein from the Golgi apparatus is an early event in brefeldin A action. J Cell Biol 1990;111:2295-2306.
67. Kirchhausen T. Adaptors for clathrin-mediated traffic. Ann Rev Cell Dev Biol 1999;15:705-732. 10.1146/annurev.cellbio.15.1.705.
68. Cheng L, Tachibana K, Iwasaki H, Kameyama A, Zhang Y, Kubota T, Hiruma T, Tachibana K, Kudo T, Guo JM, Narimatsu H. Characterization of a novel human UDP-GalNAc transferase, pp-GalNAc-T15. FEBS Lett 2004;566:17-24. 10.1016/j.febslet.2004.03.108.
69. Campbell JA, Davies GJ, Bulone V, Henrissat B. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem J 1997;326:929-939.
70. Lorente-Rodríguez A, Heidtman M, Barlowe C. Multicopy suppressor analysis of thermosensitive YIP1 alleles implicates GOT1 in transport from the ER. J Cell Sci 2009;122:1540-1550. 10.1242/jcs.042457.
71. Verrier SE, Willmann M, Wenzel D, Winter U, Fischer von Mollard G, Söling HD. Members of a mammalian SNARE complex interact in the endoplasmic reticulum in vivo and are found in COPI vesicles. Eur J Cell Biol 2008;87:863-878. 10.1016/j.ejcb.2008.07.003.
72. Hirose H, Arasaki K, Dohmae N, Takio K, Hatsuzawa K, Nagahama M, Tani K, Yamamoto A, Tohyama M, Tagaya M. Implication of ZW10 in membrane trafficking between the endoplasmic reticulum and Golgi. EMBO J 2004;23:1267-1278. 10.1038/sj.emboj.7600135.
73. Dilcher M, Veith B, Chidambaram S, Hartmann E, Schmitt HD, Fischer von Mollard G. Use1p is a yeast SNARE protein required for retrograde traffic to the ER. EMBO J 2003;22:3664-3674. 10.1093/emboj/cdg339.
74. Burri L, Varlamov O, Doege CA, Hofmann K, Beilharz T, Rothman JE, Söllner TH, Lithgow T. A SNARE required for retrograde transport to the endoplasmic reticulum. Proc Natl Acad Sci U S A 2003;100:9873-9877. 10.1073/pnas.1734000100.
75. Conchon S, Cao X, Barlowe C, Pelham HR. Got1p and Sft2p: membrane proteins involved in traffic to the Golgi complex. EMBO J 1999;18:3934-3946. 10.1093/emboj/18.14.3934.
76. Rabouille C, Hui N, Hunte F, Kieckbusch R, Berger EG, Warren G, Nilsson T. Mapping the distribution of Golgi enzymes involved in the construction of complex oligosaccharides. J Cell Sci 1995;108:1617-1627.
77. Tang BL, Low SH, Hong W. Differential response of resident proteins and cycling proteins of the Golgi to brefeldin A. Eur J Cell Biol 1995;68:199-205.
78. Haffner C, Frauli M, Topp S, Irmler M, Hofmann K, Regula JT, Bally-Cuif L, Haass C. Nicalin and its binding partner Nomo are novel nodal signaling antagonists. EMBO J 2004;23:3041-3050. 10.1038/sj.emboj.7600307.
79. Cosson P, Letourneur F. Coatomer interaction with di-lysine endoplasmic reticulum retention motifs. Science 1994;263:1629-1631. 10.1126/science.8128252.
80. Jackson MR, Nilsson T, Peterson PA. Identification of a consensus motif for retention of transmembrane proteins in the endoplasmic reticulum. EMBO J 1990;9:3153-3162.
81. Erfle H, Lisauskas T, Claas C, Reymann J, Starkuviene V. Cell arrays for the measurement of organelle dynamics in living cells. Methods Mol Biol 2011;706:73-81. 10.1007/978-1-61737-970-3_6.
82. Zilberstein A, Snider MD, Porter M, Lodish HF. Mutants of vesicular stomatitis virus blocked at different stages in maturation of the viral glycoprotein. Cell 1980;21:417-427. 10.1016/0092-8674.(80)90478-X.
83. Keller P, Toomre D, Díaz E, White J, Simons K. Multicolour imaging of post-Golgi sorting and trafficking in live cells. Nat Cell Biol 2001;3:140-149. 10.1038/35055042.
84. Nakamura N, Rabouille C, Watson R, Nilsson T, Hui N, Slusarewicz P, Kreis TE, Warren G. Characterization of a cis-Golgi matrix protein, GM130. J Cell Biol 1995;131:1715-1726.
85. Mardones GA, Snyder CM, Howell KE. Cis-Golgi matrix proteins move directly to endoplasmic reticulum exit sites by association with tubules. Mol Biol Cell 2006;17:525-538. 10.1091/mbc.E05-05-0447.
86. Miles S, McManus H, Forsten KE, Storrie B. Evidence that the entire Golgi apparatus cycles in interphase HeLa cells: sensitivity of Golgi matrix proteins to an ER exit block. J Cell Biol 2001;155:543-555. 10.1083/jcb.200103104.
87. Atkins P, Paula J. Atkins' Physical chemistry, 8th edn. Oxford University Press; 2006.
88. Liu Y, Boukhelifa M, Tribble E, Morin-Kensicki E, Uetrecht A, Bear JE, Bankaitis VA. The Sac1 phosphoinositide phosphatase regulates Golgi membrane morphology and mitotic spindle organization in mammals. Mol Biol Cell 2008;19:3080-3096. 10.1091/mbc.E07-12-1290.
89. Sevier CS, Weisz OA, Davis M, Machamer CE. Efficient export of the vesicular stomatitis virus G protein from the endoplasmic reticulum requires a signal in the cytoplasmic tail that includes both tyrosine-based and di-acidic motifs. Mol Biol Cell 2000;11:13-22.
90. Nishimura N, Balch WE. A di-acidic signal required for selective export from the endoplasmic reticulum. Science 1997;277:556-558. 10.1126/science.277.5325.556.
91. Stroud WJ, Jiang S, Jack G, Storrie B. Persistence of Golgi matrix distribution exhibits the same dependence on Sar1p activity as a Golgi glycosyltransferase. Traffic 2003;4:631-641. 10.1034/j.2003.00122.x.
92. Kasap M, Thomas S, Danaher E, Holton V, Jiang S, Storrie B. Dynamic nucleation of Golgi apparatus assembly from the endoplasmic reticulum in interphase Hela cells. Traffic 2004;5:595-605. doi:10.1111/j1398-92192004.00203.x
93. Jiang S, Storrie B. Cisternal rab proteins regulate Golgi apparatus redistribution in response to hypotonic stress. Mol Biol Cell 2005;16:2586-2596. 10.1091/mbc.E04-10-0861.
94. Starkuviene V, Pepperkok R. Differential requirements for ts-O45-G and procollagen biosynthetic transport. Traffic 2007;8:1035-1051. 10.1111/j1600-0854.2007.00582.x
95. Starkuviene V, Liebel U, Simpson JC, Erfle H, Poustka A, Wiemann S, Pepperkok R. High-content screening microscopy identifies novel proteins with a putative role in secretory membrane traffic. Genome Res 2004;14:1948-1956. doi:10.1101/gr.2658304.
96. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK. Global analysis of protein localization in budding yeast. Nature 2003;425:686-691. 10.1038/nature02026.
97. Dettmer U, Kuhn PH, Abou-Ajram C, Lichtenthaler SF, Krüger M, Kremmer E, Haass C, Haffner C. Transmembrane protein 147 (TMEM147) is a novel component of the Nicalin-NOMO protein complex. J Biol Chem 2010;285:26174-26181. 10.1074/jbc.M110.132548.
98. Rohde HM, Cheong FY, Konrad G, Paiha K, Mayinger P, Boehmelt G. The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex. J Biol Chem 2003;278:52689-52699. 10.1074/jbc.M307983200.
99. Blagoveshchenskaya A, Cheong FY, Rohde HM, Glover G, Knödler A, Nicolson T, Boehmelt G, Mayinger P. Integration of Golgi trafficking and growth factor signaling by the lipid phosphatase SAC1. J Cell Biol 2008;180:803-812. 10.1083/jcb.200708109.
100. Chen CZ, Collins RN. Insights into biological functions across species: examining the role of Rab proteins in YIP1 family function. Biochem Soc Trans 2005;33:614-618. 10.1042/BST0330614.
101. Chen CZ, Calero M, DeRegis CJ, Heidtman M, Barlowe C, Collins RN. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics 2004;168:1827-1841. 10.1534/genetics.104.032888.
102. Calero M, Winand NJ, Collins RN. Identification of the novel proteins Yip4p and Yip5p as Rab GTPase interacting factors. FEBS Lett 2002;515:89-98. 10.1016/S0014-5793.(02)02442-0
103. Yang X, Matern HT, Gallwitz D. Specific binding to a novel and essential Golgi membrane protein (Yip1p) functionally links the transport GTPases Ypt1p and Ypt31p. EMBO J 1998;17:4954-4963. 10.1093/emboj/17.17.4954.
104. Heidtman M, Chen CZ, Collins RN, Barlowe C. A role for Yip1p in COPII vesicle biogenesis. J Cell Biol 2003;163:57-69. 10.1083/jcb.200306118.
105. Barrowman J, Wang W, Zhang Y, Ferro-Novick S. The Yip1p.Yif1p complex is required for the fusion competence of endoplasmic reticulum-derived vesicles. J Biol Chem 2003;278:19878-19884. 10.1074/jbc.M302406200.
106. Kano F, Yamauchi S, Yoshida Y, Watanabe-Takahashi M, Nishikawa K, Nakamura N, Murata M. Yip1A regulates the COPI-independent retrograde transport from the Golgi complex to the ER. J Cell Sci 2009;122:2218-2227. 10.1242/jcs.043414.
107. Dykstra KM, Pokusa JE, Suhan J, Lee TH. Yip1A structures the mammalian endoplasmic reticulum. Mol Biol Cell 2010;21:1556-1568. 10.1091/mbc.E09-12-1002.
108. Yoshida Y, Suzuki K, Yamamoto A, Sakai N, Bando M, Tanimoto K, Yamaguchi Y, Sakaguchi T, Akhter H, Fujii G, Yoshimura S-I, Ogata S, Sohda M, Misumi Y, Nakamura N. YIPF5 and YIF1A recycle between the ER and the Golgi apparatus and are involved in the maintenance of the Golgi structure. Exp Cell Res 2008;314:3427-3443. 10.1016/j.yexcr.2008.07.023.
109. Shakoori A, Fujii G, Yoshimura SI, Kitamura M, Nakayama K, Ito T, Ohno H, Nakamura N. Identification of a five-pass transmembrane protein family localizing in the Golgi apparatus and the ER. Biochem Biophys Res Commun 2003;312:850-857. 10.1016/j.bbrc.2003.10.197.
110. Heidtman M, Chen CZ, Collins RN, Barlowe C. Yos1p is a novel subunit of the Yip1p-Yif1p complex and is required for transport between the endoplasmic reticulum and the Golgi complex. Mol Biol Cell 2005;16:1673-1683. 10.1091/mbc.E04-10-0873.
111. Marie M, Dale HA, Sannerud R, Saraste J. The function of the intermediate compartment in pre-Golgi trafficking involves its stable connection with the centrosome. Mol Biol Cell 2009;20:4458-4470. 10.1091/mbc.E08-12-1229.
112. Kano F, Sako Y, Tagaya M, Yanagida T, Murata M. Reconstitution of brefeldin A-induced Golgi tubulation and fusion with the endoplasmic reticulum in semi-intact chinese hamster ovary cells. Mol Biol Cell 2000;11:3073-3087.
113. Scales SJ, Pepperkok R, Kreis TE. Visualization of ER-to-Golgi transport in living cells reveals a sequential mode of action for COPII and COPI. Cell 1997;90:1137-1148. 10.1016/S0092-8674.(00)80379-7
114. Soille P. Morphological Image Analysis: Principles and Applications, 2nd edn. New York: Springer-Verlag; 2003.
115. Zack GW, Rogers WE, Latt SA. Automatic measurement of sister chromatid exchange frequency. J Histochem Cytochem 1977;25:741-753.